Effect of surface roughness and composition of titanium on proliferation and differentiation of osteoblasts

Abstract

Abstract: PURPOSE: To study the effects of surface roughness and composition of titanium on proliferation and differentiation of osteoblasts. METHODS: Osteoblasts were cultured on 5 commercially pure titanium (cp Ti) substrates of ground (S0), blasted with 108～130 μm(S1), 216～301 μm(S2), 356～411 μm (S3) TiO2 particles and titanium-sprayed plasma(TPS) surfaces. Surfaces prepared by hand grinding with SiC paper of 600 grits served as control (S0). Electron microprobe was used to evaluate the TiO2 film structure of the 5 titanium surfaces. For proliferation and differentiation measurement, osteoblasts were cultured for 1, 3, 5 and 7 days，evaluated by MTT Assay, ALP activity and OC level. SPSS12.0 software package was used for one-way ANOVA. RESULTS: The number of cells was the highest on S3 and TPS surfaces after 1 day culture. The same results were observed after 5 days. On day 3 and 7, S3 surface was the highest(P<0.05). The number of cells on all experimental groups were higher than S0 surfaces at each time point(P<0.05). Increase of ALP activity were detected on S0, S1 and S2 surfaces after 1, 3, 5 days. However, there was no difference between S3 and TPS surfaces(P>0.05). After 7 days, ALP activity increased significantly on TPS surface than on S1 or S2 surfaces. ALP activity on S3 surfaces was the highest(P<0.05). The ALP activity on all experimental groups were higher than S0 surfaces at each time point(P<0.05). An increase in OC production was detected on S0, S1, S2 and S3 surfaces after 1, 3, 5 days. The highest OC production was on S3 surface was on day 7(P<0.05). CONCLUSIONS: It was concluded that for TiO2 blasted surfaces, Ra ranging from 1.260 μm to 3.530 μm would optimize proliferation and differentiation of osteoblasts. Blasting was an effective treatment method for osteointegration in vitro.

Key words: Osteoblast, Sandblasted, Titanium-sprayed-plasma, Proliferation, Differentiation

CLC Number:

R783.6

ZHOU Yi-li, DING Zhong-juan, TANG Ling. Effect of surface roughness and composition of titanium on proliferation and differentiation of osteoblasts[J]. Shanghai Journal of Stomatology, 2013, 22(3): 282-286.

References

[1] Velten D, Biehl V, Aubertin F, et al. Preparation of TiO2 layers on cp-Ti and Ti6Al4V by thermal and anodic oxidation and by sol-gel coating techniques and their characterization[J]. J Biomed Mater Res, 2002, 59(1): 18-28.
[2] Wirth C, Grosgogeat B, Lagneau C, et al. Biomaterial surface properties modulate in vitro rat calvaria osteoblasts response: roughness and or chemistry?[J]. Mater Sci Eng C, 2008, 28(5-6): 990-1001.
[3] Imamura K, Shimomura M, Nagai S, et al. Adsorption characteristics of various proteins to a titanium surface[J]. J Biosci Bioeng, 2008, 106(3), 273-278.
[4] Boyan BD, Bonewald LF, Paschalis EP, et al. Osteoblast-mediated mineral deposition in culture is dependent on surface microtopography[J]. Calcif Tissue Int, 2002, 71(6): 519-529.
[5] Rungsiyakull C, Li Q, Sun G, et al. Surface morphology optimization for osseointegration of coated implants[J]. Biomaterials, 2010, 31(27): 7196-7204.
[6] Li DH, Liu BL, Zou JC, et al. Improvement of osseointegration of titanium dental implants by a modified sandblasting surface treatment: an in vivo interfacial biomechanics study[J]. Implant Dent, 1999, 8(3): 289-294.
[7] Walivaara B, Aronssob BO, Rodhal M, et al. Titanium with different oxides: in vitro studies of protein adsorption and contact activation[J]. Biomaterials, 1994, 15(10): 827-834.
[8] Kasemo B, Lausmaa J. Surface science aspects on inorganic biomaterials[J]. Crit Rev Biocomp, 1986, 2:335-380.
[9] Postiglione L, Di Domenico G, Ramaglia L, et al. Different titanium surfaces modulate the bone phenotype of SaOS-2 osteoblast-like cells[J]. Eur J Histochem, 2004, 48(3): 213-222.
[10] Deligianni DD, Katsala N, Ladas S, et al. Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption[J]. Biomaterials, 2001, 22(11): 1241-1251.
[11] Marie PJ. Human endosteal osteoblastic cells: relationship with bone formation[J]. Calcif Tissue Int, 1995, 56 (Suppl 1): S13-16.
[12] 周屹立, 丁仲鹃, 唐玲. 钛片表面粗糙度和氧化膜对成骨细胞黏附影响的体外研究[J]. 华西口腔医学杂志,2011,29(1):13-16.
[13] Mustafa K, Rubinstein J, Lopez BS, et al. Production of transforming growth factor beta1 and prostaglandin E2 by osteoblast-like cells cultured on titanium surfaces blasted with TiO2 particles[J]. Clin Oral Implants Res, 2003, 14(1): 50-56.
[14] Wu Y, Zitelli JP, TenHuisen KS, et al. Differential response of Staphylococci and osteoblasts to varying titanium surface roughness[J]. Biomaterials, 2011, 32(4): 951-960.
[15] Mustafa K, Wennerberg A, Wroblewski J, et al. Determining optimal surface roughness of TiO2 blasted titanium implant material for attachment, proliferation and differentiation of cells derived from human mandibular alveolar bone[J]. Clin Oral Implants Res, 2001, 12(5): 515-525.
[16] Rosa AL, Beloti MM. Effect of cpTi surface roughness on human bone marrow cell attachment, proliferation, and differentiation[J]. Braz Dent J, 2003, 14(1):16-21