Effect of recombinant connective tissue growth factor on proliferation and odontogenic differentiation of dental pulp cells

doi:10.19439/j.sjos.2020.01.002

Abstract

Abstract: PURPOSE: To analyze the effect of recombinant connective tissue growth factor(rCTGF) on proliferation and differentiation of human dental pulp cells(hDPCs). METHODS: Human dental pulp cells were cultured in vitro and treated with rCTGF at different concentrations (0, 1, 10, 100 ng/mL). The proliferation of dental pulp cells was detected by CCK8 assay. The formation of mineralized nodules was determined by alizarin red staining and half-quantitative alizarin Red S assay. qRT-PCR was utilized to detect the expression of odontogenic differentiation related genes DMP-1, DSPP and OC, and the phosphorylation level of ERK1/2 signaling pathway was detected by Western blot. The data were analyzed with SAS 9.3 software package. RESULTS: High concentration of rCTGF(100 ng/mL) could promote proliferation of dental pulp cells. After mineralization induction, 10 g/mL rCTGF had the best effect on promoting the formation of mineralized nodules in dental pulp cells, and calcium ion deposition was the most obvious(P<; 0.05). The expression of odontogenic differentiation related genes DMP-1 and DSPP was significantly up-regulated(P<; 0.05). Western blot results showed that hDPCs stimulated by 10 ng/mL rCTGF could increase the expression of p-ERK1/2. CONCLUSIONS: rCTGF may promote the proliferation and differentiation of human dental pulp cells through activating ERK1/2 signaling pathway.

Key words: Recombinant connective tissue growth factor, Human dental pulp cells, Cell proliferation, Cell differentiation, ERK1/2 signaling pathway

CLC Number:

R781.3

CAO Ying, PENG Wei-wei, WANG Xuan, WU Tian-tian, DU Rong, ZHU Ya-qin. Effect of recombinant connective tissue growth factor on proliferation and odontogenic differentiation of dental pulp cells[J]. Shanghai Journal of Stomatology, 2020, 29(1): 7-12.

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