Experimental study on transplantation of MIRB labeled human deciduous dental pulp stem cells to repair periodontal bone defects in rats

doi:10.19439/j.sjos.2022.06.004

Abstract

Abstract: PURPOSE: To trace the fate of transplanted stem cells from human exfoliated deciduous teeth (SHED) in the repair of periodontal bone defects, Molday ION rhodamine B (MIRB) was used to label SHED and explore the mechanism of SHED in the repair of periodontal bone defects. METHODS: In vitro cultured SHED were labeled by MIRB. The labeling efficiency, cell survival, proliferation and osteogenic differentiation of MIRB-labelled SHED were detected. The labeled cells were transplanted into the rat model with periodontal bone defect. The survival, differentiation and improvement of host periodontal bone healing of MIRB labeled SHED in vivo were analyzed by immunohistochemistry and fluorescence co-staining, nuclear magnetic imaging dual-mode tracking and H-E staining. The data were statistically analyzed with SPSS 24.0 software package. RESULTS: MIRB labeled SHED did not affect its growth and osteogenic differentiation. The optimal labeling concentration was 25 μg/mL, the labeling efficiency of SHED reached 100%. The transplantation of MIRB labeled SHED in vivo can survive for more than 8 weeks. It was found that MIRB labeled SHED could differentiate into osteoblasts in vivo and significantly promote the repair of alveolar bone defects. CONCLUSIONS: MIRB labeled SHED can be traced in vivo, and the effect of labeled SHED on the repair of defective alveolar bone was observed.

Key words: Stem cells from human exfoliated deciduous teeth, MIRB, In vivo tracking, Nuclear magnetic image, Periodontal bone reconstruction

CLC Number:

R780

XU Li, BU Chun-qing, KANG Jie, JIA Guo-tao, SONG Hao, ZHANG Chuan-chen, ZHANG Nan, HAN Fa-bin. Experimental study on transplantation of MIRB labeled human deciduous dental pulp stem cells to repair periodontal bone defects in rats[J]. Shanghai Journal of Stomatology, 2022, 31(6): 581-587.

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