Significance of Smad4, Smad7 and Caveolin-1 expression in oral mucosa carcinogenesis of Wistar rats

doi:10.19439/j.sjos.2017.04.012

Abstract

Abstract: To investigate the expression of Smad4, Smad7 and Caveolin-1 in the process of carcinogenesis of oral mucosa in Wistar rats, and to understand the changes of TGF-β/Smad signaling pathway and Caveolin-1 in oral cancer. METHODS: Palatal mucosal carcinogenesis specimens of Wistar rats were obtained from School of Stomatology, Zhengzhou University, which included 5 samples of normal mucosa, 10 samples of simple hyperplasia mucosa, 6 samples of mild mucosal dysplasia, 7 samples of moderate mucosal dysplasia, 13 samples of mucosa severe mucosal dysplasia, and 28 samples of oral cancer tissue. The expression of Smad4, Smad7 and Caveolin-1 was detected by immunohistochemistry. SPSS15.0 software package was used for statistical analysis. RESULTS: The expression of Smad4 decreased in normal and hyperplastic epithelia, dysplasticepithelia and oral cancer gradually, the difference of the expression among the three groups was significant (P<0.05). The expression of Smad7 and Caveolin-1 increased in normal and hyperplastic epithelia, dysplasticepithelia and oral cancer gradually, respectively; the difference of the expression among the 3 groups was significant (P<0.05). Spearman correlation analysis showed that Smad4 was negatively correlated with Smad7, Smad4 was negatively correlated with caveolin-1, Smad7 was positively correlated with Caveolin-1 (P<0.05). CONCLUSIONS: Synergistic effects may exist among Smad4, Smad7 and caveolin-1 in carcinogenesis of oral cancer.

Key words: Oral cancer, Dysplasticepithelia, Smad4, Smad7, Caveolin-1

CLC Number:

R781.5

LI Yuan, ZHOU Jian-wei, LI Hui-liang, LIU Jin-zhong. Significance of Smad4, Smad7 and Caveolin-1 expression in oral mucosa carcinogenesis of Wistar rats[J]. Shanghai Journal of Stomatology, 2017, 26(4): 409-413.

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