Effect of low-intensity pulsed ultrasound combined with triamcinolone acetonide on oral mucosal ulcer healing in syrian hamster

doi:10.19439/j.sjos.2017.04.006

Abstract

Abstract: To evaluate the effect of low-intensity pulsed ultrasound（LIPUS） combined with triamcinolone acetonide on oral mucosal ulcer in syrian hamster in several ways, including healing time, contents of superoxide dismutase（SOD）and malondialdehyde (MDA). METHODS: Sixty syrian hamsters were randomly divided into 5 groups, including a baseline group (containing a normal baseline group and a model baseline group, n=6) and 4 experimental groups (LIPUS processing and drug use group, LIPUS group, drug group and a normal control group without any processing, n=12). Four experimental groups and model baseline group were given oxygen free radicals to model the oral mucosal ulcer. At 24 h after the last treatment, the healing time of ulcer, content of SOD and MDA were compared between each group. SPSS 20.0 software package was used for statistical analysis. RESULTS: Compared with LIPUS group,drug group and control group, the healing time of oral mucosal ulcer in LIPUS and drug combined group was shortened. At 24 h after the last treatment, the activity of SOD showed that the LIPUS and drug combined group[(2.32±0.30) U/mgprot] were significantly higher than the model baseline group[(1.48±0.29) U/mgprot], the LIPUS group[(1.83±0.15) U/mgprot], the drug group[(1.76±0.25) U/mgprot] and control group[(1.71±0.18) U/mgprot] (P<0.05). The results of MDA content showed that the LIPUS and drug combined group [(8.17±0.21) nmol/mgprot] were significantly lower than the model baseline group[(9.41±0.22) nmol/mgprot], the LIPUS group[(9.00±0.44) nmol/mgprot], the drug group [(9.04±0.43) nmol/mgprot] and control group[(9.03±0.46) nmol/mgprot] (P<0.05). After oral mucosal ulcer healing, the activity of SOD and MDA showed that the LIPUS and drug combined group, the LIPUS group, the drug group and control group were not significantly different from the normal baseline group (P>0.05). CONCLUSIONS: Low-intensity pulsed ultrasound combined with triamcinolone acetonide can effectively improve the activity of SOD and reduce the contents of MDA in ulcerated tissues, and therefore accelerate the process of ulcer healing．

Key words: Low-intensity pulsed ultrasound, Triamcinolone acetonide, Oral ulcer

FENG Tian-ming, YANG Ming-cong, PAN Lan-lan, FEI Chen-xi, WANG Chao, XIANG Xue-rong, SUN Jun. Effect of low-intensity pulsed ultrasound combined with triamcinolone acetonide on oral mucosal ulcer healing in syrian hamster[J]. Shanghai Journal of Stomatology, 2017, 26(4): 379-383.

References

[1] 陈谦明. 口腔黏膜病学[M]. 第4版. 北京: 人民卫生出版社, 2012: 64-65.
[2] Vale FA, Moreira MS, de Almeida FC. Low-level laser therapy in the treatment of recurrent aphthous ulcers: a systematic review [J]. Sci World J, 2015, 2015: 150412.
[3] Messadi DV, Younai F. Aphthous ulcers[J]. Dermatol Ther, 2010, 23(3): 281-290.
[4] Khanna A, Nelmes RT, Gougoulias N, et al.The effects of LIPUS on soft-tissue healing: a review of literature[J]. Br Med Bull, 2009, 89: 169-182.
[5] Harrison A, Lin S, Pounder N, et al. Mode & mechanism of low- intensity pulsed ultrasound(LIPUS) in fracture repair [J]. Ultrasonics, 2016, 70: 45-52.
[6] 向学熔, 季平, 范小平, 等. 低频超声治疗口腔溃疡的实验研究的实验研究 [J]. 第三军医大学学报, 2012, 34(12): 1214-1216.
[7] 郑利光,华红.曲安奈德外用治疗口腔黏膜病的研究进展 [J].实用口腔医学杂志, 2015,31(2): 278-281.
[8] 王学莹, 关为群. 脂氧素A4及相关炎症因子在金黄地鼠口腔黏膜溃疡病灶中的表达 [J].口腔医学研究, 2013,29(8): 699-703.
[9] Maeda T, Masaki C, Kanao M, et al. Low-intensity pulsed ultrasound enhances palatal mucosa wound healing in rats [J]. J Prosthodont Res, 2013, 57(2): 93-98.
[10] Min BH, Woo JI, Cho HS, et al. Effects of low-intensity ultrasound (LIUS) stimulation on human cartilage explants [J]. Scand J Rheumatology, 2006, 35(4): 305-311.
[11] Iwanabe Y, Masaki C, Tamura A, et al. The effect of low-intensity pulsed ultrasound on wound healing using scratch assay in epithelial cells [J]. J Prosthodont Res, 2016, 60(4): 308-314.
[12] Lim YS, Kwon SK, Park JH, et al. Enhanced mucosal healing with curcumin in animal oral ulcer model [J]. Laryngoscope, 2016, 126(2): E68-73.
[13] Yu ZH, Jin C, Xin M, et al. Effect of Aloe vera polysaccharides on immunity and antioxidant activities in oral ulcer animal models [J]. Carbohydr Polym, 2009, 75(2): 307-311.
[14] 赵志宇, 郭兰. 槲皮素壳聚糖膜对口腔溃疡及创面组织中超氧化物歧化酶和丙二醛含量的影响 [J]. 中国组织工程研究, 2012, 16(29): 5437-5440.
[15] 杨苏平, 刘鲁川. 脉冲Nd:YAG激光照射剂量对口腔溃疡组织中SOD活性和MDA含量的影响 [J]. 牙体牙髓牙周病学杂志, 2009, 19(1): 32-34, 41.
[16] Miyasaka M, Nakata H, Hao J, et al. Low-intensity pulsed ultrasound stimulation enhances Heat-shock protein 90 and mineralized nodule formation in mouse calvaria-derived osteoblasts [J]. Tissue Eng Part A, 2015, 21(23-24): 2829-2839.
[17] Shiraishi R, Masaki C, Toshinaga A, et al. The effects of low-intensity pulsed ultrasound exposure on gingival cells [J]. J Periodontol, 2011, 82(10): 1498-1503.
[18] 国家药典委员会. 中国药典临床用药须知(化学药和生物制品卷) [M]. 北京: 中国医药科技出版社, 2011: 606, 1449.
[19] 郭芳, 胡兵. 低频超声的应用基础研究进展 [J]. 声学技术, 2012, 31(2): 198-203.
[20] 孙必强, 张鸣. 威灵仙注射液关节腔离子导入对骨关节炎 NO、SOD 和 PGE2 的影响[J]. 中国民族民间医药, 2014, 23(9): 6-7.
[21] Tang H, Mitragotri S, Blankschtein D, et al. Theoretical description of transdermal transport of hydrophilic permeants: application to low-frequency sonophoresis [J]. J Pharm Sci, 2001, 90(5): 545-568.