Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue

doi:10.19439/j.sjos.2017.04.004

Shanghai Journal of Stomatology ›› 2017, Vol. 26 ›› Issue (4): 368-373.doi: 10.19439/j.sjos.2017.04.004

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Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue

ZHAI Yue¹, HUANG Jian-ying², HYUN Park¹, JI Fang³, FEI Zhao-liang⁴, TAO Jiang¹

1.Department of General Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011;
2.Central Laboratory, Center for Neonatal Disease Screening, Maternal and Child Health Hospital of Jiangxi Province. Nanchang 330002, Jiangxi Province;
3.Department of Orthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011;
4.Institute of Biochemistry and Cell Biology,Shanghai Institute for Biological Sciences,Chinese Academy of Sciences.Shanghai 200031, China

Received:2017-02-15 Revised:2017-04-06 Online:2017-08-25 Published:2017-09-01

Abstract

Abstract: To investigate the expression of human β-defensin(HBD) in human dental pulp tissue and to explore the regulation of HBD in pulp inflammation and the relationship among HBD family members. METHODS: The gene expression of HBD in human dental pulp tissue was assessed in NCBI GEO profiles and was verified by RT-PCR. Human dental pulp cells were stimulated with TNF-α, IL-1α, IL-1β and IL-6 in different combinations and the expression of HBD2 was analyzed by qPCR. Human dental pulp cells were pretreated with HBD110 and then stimulated with LPS and the expression of TNF-α,IL-1α and HBD2 were analyzed by qPCR. GraphPad Prism 5.01 was used to analyze the results of the experimental and the control groups. RESULTS: 27 HBDs were found to express in human dental pulp tissue in NCBI GEO Profiles. The joint overexpression of TNF-α, IL-1α, IL-1β and IL-6 increased the expression of HBD2; HBD110 increased the expression of HBD2 by increasing the expression of TNF-α and IL-1α. CONCLUSIONS: Many other HBDs have positive expression in human dental pulp issue besides of HBD1, HBD2, HBD3, HBD4 and the inflammation factors and other HBDs can regulate the expression of HBD2 in dental pulp.

Key words: Human β, -defensin, Dental pulp, Inflammation

CLC Number:

R781.3

ZHAI Yue, HUANG Jian-ying, HYUN Park, JI Fang, FEI Zhao-liang, TAO Jiang. Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue[J]. Shanghai Journal of Stomatology, 2017, 26(4): 368-373.

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Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue

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