上海口腔医学

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (1): 46-51.

• 基础研究 • 上一篇    下一篇

纯钛表面涂层（Ca+Zn）/P比值与成骨细胞生物活性的关系

商亚微1,张伟2,何晶1,王丹宁1,钟鸣3,赵宝红1   

  1. 1.中国医科大学附属口腔医院 种植中心,辽宁省口腔医学研究所 口腔种植研究室,辽宁省口腔疾病转化医学研究中心,辽宁 沈阳 110002;
    2.中国科学院 金属腐蚀与防护研究所,辽宁 沈阳 110016;
    3.中国医科大学口腔医学院 中心实验室,辽宁 沈阳 110002
  • 收稿日期:2014-05-12 出版日期:2015-02-20 发布日期:2015-07-24
  • 通讯作者: 赵宝红,Tel：024-22891221,E-mail:zhao_baohong@vip.sohu.com
  • 作者简介:商亚微（1987-）,女,硕士,医师,E-mail:shangyawei_1234@163.com
  • 基金资助:
    辽宁省自然科学基金（20092093）; 辽宁省科技计划项目（2013225049）; 沈阳市科技计划项目（F11-264-1-25）

Study of relationship between (Ca+Zn)/P of pure titanium surface coating and biological activity of osteoblasts

SHANG Ya-wei1,ZHANG Wei2,HE Jing1,WANG Dan-ning1,ZHONG Ming3,ZHAO Bao-hong1   

  1. 1.Oral Implanting Center of Hospital of Stomatology, China Medical University; Oral Implanting Laboratory, Oral Medical Research Institute of Liaoning Province, Translational Medicine Research Center of Oral Disease of Liaoning Province. Shenyang 110002;
    2. Institute of Metal Corrosion and Protection, China Academy of Sciences. Shenyang 110016;
    3. Central Laboratory, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China
  • Received:2014-05-12 Online:2015-02-20 Published:2015-07-24
  • Supported by:
    Natural Science Foundation of Liaoning Province (20092093), Science and Technology Project of Liaoning Province (2013225049), and Science and Technology Project of Shenyang City (F11-264-1-25)

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摘要： 目的研究纯钛表面不同（Ca+Zn）/P比值对成骨细胞（MC3T3-E1）黏附、增殖和分化的影响,确定纯钛表面生物活性最佳的Ca、P、Zn含量。方法在微弧氧化的电解液中加入一定浓度的Ca、P和5种不同浓度的Zn（0、0.01、0.03、0.04和0.06 mol/L）,使Ca、P元素的摩尔比值接近羟基磷灰石的比值。在纯钛材料表面制备5种不同（Ca+Zn）/P比值的生物活性涂层,分别记为S0、S1、S2、S3和S4组。应用XPS和SEM分析样品表面的元素组成、存在形式、（Ca+Zn）/P比值以及表面形貌;应用SEM、MTT法和ALP活性测定法分析材料表面培养的MC3T3-E1细胞黏附、增殖和分化情况。采用SPSS13.0软件包对数据进行统计学分析。结果随着电解液中Zn浓度的增加,S0~S4组（Ca+Zn）/P比值分别为2.94、2.64、2.71、2.87和2.72,其中,在S3组时达到最大,S4组时降低。MC3T3-E1细胞在5组材料表面黏附、增殖和分化能力,依次为S3>S4>S2>S1>S0,各组之间差异具有显著性（P<0.05）。结论应用微弧氧化法在纯钛表面制备的不同含量Ca、P、Zn涂层,随着（Ca+Zn）/P比值的增大,成骨细胞的黏附、增殖和分化能力随之增强。其中,（Ca+Zn）/P比值为2.87时,细胞的生物活性最佳。

关键词: 微弧氧化, 钛, 锌, 钙, 磷, 成骨细胞

Abstract: PURPOSE: To study osteoblast (MC3T3-E1 cells) adhesion, proliferation and differentiation activity on the surface of pure titanium with different (Ca+Zn)/P ratios,and determine Ca,P,Zn contents offering best biological activity on pure titanium surface. METHODS: In the micro-arc oxidation electrolyte, Ca, P with a certain concentration, and 5 different concentrations of Zn (0,0.01,0.03,0.04, and 0.06 mol/L) were added, which made the mole ratio of Ca and P element close to the ratio of hydroxyapatite. (Ca+Zn)/P ratio of bioactive coating on 5 different pure titanium material surfaces were prepared, which named S0 and S1, S2, S3 and S4 group, respectively. The surface elements, existence form, (Ca + Zn)/P ratio and surface morphology were analyzed by XPS and SEM; MC3T3-E1 cells adhesion, proliferation and differentiation cultured on the material surface were analyzed by SEM, MTT method and ALP activity assay. RESULTS: The data was analyzed with SPSS13.0 software package. With the increase of Zn content in the electrolyte, (Ca+Zn)/P ratios were 2.94, 2.64 2.71,2.87 and 2.72 in S0 ~ S4 group, respectively. (Ca+Zn)/P ratio was maximal in S3 group and decreased in S4 group. MC3T3-E1 cell adhesion, proliferation and differentiation capacity on material surface of 5 groups changed in the following order: S3 > S4 > S2 > S1 > S0, with significant differences among 5 groups (P<0.05). CONCLUSIONS: Coating with different concentrations of Ca, P, Zn on pure titanium surface by micro-arc oxidation technique, the adhesion, proliferation and differentiation ability of osteoblast increase as (Ca+Zn)/P ratio increase, and cell biological activity is the best when the (Ca+Zn)/P ratio reached 2.87.

Key words: Micro-arcoxidation, Titanium, Zinc, Calcium, Phosphorus, Osteoblast

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