微种植钉周期性加力压低犬磨牙牙周组织中MMP-2的表达

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (1): 41-45.

微种植钉周期性加力压低犬磨牙牙周组织中MMP-2的表达

顾梦婕,公柏娟,孙新华,王恩辉,张兵,孙晗

吉林大学口腔医院,吉林长春 130021

收稿日期:2013-12-06 出版日期:2015-02-20 发布日期:2015-07-24
通讯作者: 孙新华,Tel:0431-88796023,E-mail:xinhuasun8@163.com
作者简介:顾梦婕(1989-),女,硕士,E-mail:gumengjie198909@126.com,现工作单位为安徽医科大学第一附属医院口腔科
基金资助:
吉林大学青年创新基金(450060491133)

Expression of MMP-2 in the periodontal tissues with the miniscrew for molar intrusion with cyclic intrusive force in dogs

GU Meng-jie,GONG Bai-juan,SUN Xin-hua,WANG En-hui,ZHANG Bing,SUN Han

School of Stomatology, Jilin University. Changchun 130021, Jilin Province, China

Received:2013-12-06 Online:2015-02-20 Published:2015-07-24
Supported by:
Youth Innovation Fundation of Jilin University (450060491133)

摘要/Abstract

摘要： 目的使用微种植钉压低犬磨牙,观察周期性加力不同时间牙周组织中MMP-2的表达。方法选取健康成年雄性纯种Beagle犬3只,于左侧上颌第二、第三前磨牙颊、腭侧根分叉区各植入1颗微种植钉,施加150 g的力压低犬牙;每4周加力1次。左侧磨牙作为实验组,随机分为3组,分别加力12、24、36周,右侧磨牙为空白对照组。处死动物后,取上颌第二、第三前磨牙及其周围牙周组织,固定、脱钙及制作组织学标本,切片后行MMP-2免疫组织化学染色并光镜下观察,IPP软件计算MMP-2的平均吸光度值。采用SPSS17.0 软件包对数据进行统计学分析。结果免疫组织化学染色结果显示,对照组犬磨牙牙周组织中MMP-2仅有少量表达;实验组MMP-2的表达量显著升高;压低24周,牙周组织中MMP-2的表达量达最高;压低36周表达量下降,但与对照组仍有显著性差异;压低12、24、36周组间相比,差异均无显著性（P>0.05）。结论MMP-2参与了犬牙压低过程中牙周组织的改建;在周期性加力过程中,牙周组织中MMP-2的表达没有随着压低时间的延长而明显升高。证明使用微种植钉周期性加力压低磨牙,磨牙牙周组织保持一种动态平衡的骨改建过程。

关键词: 微种植钉, 磨牙压低, MMP-2

Abstract: PURPOSE: To observe the expression of MMP-2 in the periodontal tissues of molars in Beagle dogs after different times of intrusion by miniscrew with cyclic intrusive force. METHODS: Three mature Beagle dogs were used for the experiment. On the buccal and palatal sites of the left maxillary second and third premolars, a miniscrew was placed at the interradicular septa separately, intruding the tooth with 150 g initial force, which would be reinforced every 4 weeks. The teeth of left side as the experimental group were divided into 3 subgroups, as being intruded 12, 24 and 36 weeks, and the right side as blank control. Then the dogs were sacrificed, and the second and the third premolars with the surrounding periodontal tissue were cut down, fixed, decalcified, wrapped and cut into slices. Immunohistochemical staining with MMP-2 was performed. The average optical density (OD) of MMP-2 was calculated with IPP software, which represented the expression of MMP-2. SPSS 17.0 software package was used for statistical analysis. RESULTS: Immunohistochemical staining revealed that the expression of MMP-2 in control group was low. After tooth intrusion, MMP-2 expression significantly increased in the periodontal tissues of molars, and reached the maximum in the group of 24 weeks. Then MMP-2 expression decreased in the 36-week group but still significantly higher than the control group. There was no significant difference among the 3 subgroups for different intrusion times(P>0.05). CONCLUSIONS: MMP-2 participates in remodeling of the periodontal tissue during tooth intrusion. The expression of MMP-2 is not significantly increased with the extension of the intrusion time with cyclic intrusive force, which suggests that with the use of miniscrew to intrude the molars with cyclic intrusive force, the periodontal tissues of the intruded tooth maintain dynamic balance of bone remodeling.

Key words: Miniscrew, Intruding posterior tooth, MMP-2

中图分类号:

R783.5

顾梦婕,公柏娟,孙新华,王恩辉,张兵,孙晗. 微种植钉周期性加力压低犬磨牙牙周组织中MMP-2的表达[J]. 上海口腔医学, 2015, 24(1): 41-45.

GU Meng-jie,GONG Bai-juan,SUN Xin-hua,WANG En-hui,ZHANG Bing,SUN Han. Expression of MMP-2 in the periodontal tissues with the miniscrew for molar intrusion with cyclic intrusive force in dogs[J]. Shanghai Journal of Stomatology, 2015, 24(1): 41-45.

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