上海口腔医学

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (2): 172-176.

• 论著 • 上一篇    下一篇

羧甲基壳聚糖锌多肽复合材料局部应用对巴马小型猪龈沟液中IL-1、TNF-α和PGE-2含量的影响

刘学玉1*，潘克清2，张丽2，邓婧2，郭珲2，宋佳2，刘万顺3，宋福来4   

  1. 1.青岛大学,山东 青岛 266071；
    2.青岛大学附属医院 口腔内科,山东 青岛 266003；
    3.中国海洋大学海洋生命学院 海洋生物工程系,山东 青岛 266003；
    4.青岛博益特生物材料有限公司 研发中心,山东 青岛 266003
  • 收稿日期:2015-11-11 修回日期:2016-01-14 出版日期:2016-03-25 发布日期:2016-05-06
  • 作者简介:刘学玉(1989-),女,硕士研究生,E-mail:593866072@qq.com 邓婧,Tel: 0532-82911397,E-mail:dengjing3333@126.com
  • 基金资助:
    青岛市科技局基金项目(13-1-3-60-nsh)

The effect of carboxymethyl chitosan zinc and peptide on IL-1, TNF-α and PGE-2 in gingival crevicular fluid of miniature pigs

LIU Xue-yu1, PAN Ke-qing2, ZHANG Li2, DENG Jing2, GUO Hun2, SONG Jia2, LIU Wan-shun3, SONG Fu-lai4   

  1. 1.Qingdao University. Qingdao 266071;
    2.Department of Oral Medicine, The Affiliated Hospital of Qingdao University. Qingdao 266003;
    3. Institute of Marine Life, China Ocean University. Qingdao 266003;
    4. Qingdao Boyite Biological Material Co. Qingdao 266003, Shandong Province, China
  • Received:2015-11-11 Revised:2016-01-14 Online:2016-03-25 Published:2016-05-06

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摘要： 目的:探讨羧甲基壳聚糖锌多肽复合材料(carboxymethyl chitosan zinc and peptide,CMC-Zn+-P)能否有效减缓牙周组织炎症的发生及发展,寻求一种能够有效防治牙周病的新型材料。方法:以巴马小型猪为实验对象,随机分为4组,空白对照组、羧甲基壳聚糖锌多肽复合材料组(材料组)、刷牙组以及刷牙+羧甲基壳聚糖锌多肽复合材料组(复合组)。收集实验起始时及实验进行1个月后的龈沟液,以酶联免疫吸附法(ELISA)检测龈沟液中IL-1、TNF-α和PGE-2的含量,同时记录临床牙周指数;将得到的数据录入SPSS18.0软件包进行统计学分析。结果:实验起始时,各组中IL-1、TNF-α和PGE-2的含量及临床检查指标均无显著差异。实验1个月后,复合组中IL-1、TNF-α和PGE-2的含量均显著低于其他3组(P<0.008);刷牙组和材料组的IL-1、TNF-α和PGE-2含量显著低于空白对照组(P<0.008);刷牙组中,IL-1的含量显著低于材料组(P<0.008),PGE-2的含量显著高于材料组(P<0.008),而TNF-α的含量无显著差异(P>0.008)。另外,4组之间的牙结石指数差异显著(P<0.05),其中,复合组牙结石指数显著低于其他3组,刷牙组和材料组显著低于空白对照组。结论:羧甲基壳聚糖锌多肽复合材料能够有效减轻牙周组织炎症,减缓牙结石沉积,与刷牙配合效果更佳。

关键词: 小型猪, 羧甲基壳聚糖锌多肽复合材料, IL-1, TNF-α, PGE-2

Abstract: PURPOSE:This experiment was aimed at exploring whether carboxymethyl chitosan zinc and peptide (CMC-Zn+-P) can reduce the occurrence and development of periodontal tissue inflammation effectively by observing the change of IL-1,TNF-α and PGE-2 level in gingival crevicular fluid (GCF) before and after brushing, so as to find a new effective material in preventing and treating periodontal diseases. METHODS:Miniature pigs were selected as experimental subjects and divided into 4 groups randomly: the control group; CMC-Zn+-P group (material group);brushing group; brushing + CMC-Zn+-P group (composite group). Gingival crevicular fluid before and one month after the experiment was collected. The levels of IL-1, TNF-α and PGE-2 were examined by enzyme-linked immune-sorbent assay, while the clinical periodontal index was recorded. SPSS 18.0 software package was used for statistical analysis. RESULTS:There was no significant difference in levels of IL-1, TNF-α and PGE-2 and clinical periodontal index between the 4 groups before experiment. After one month, the levels of IL-1, TNF-α, PGE-2 in GCF had significant difference between 4 groups. The levels of IL-1, TNF-α, PGE-2 in composite group were significant lower than that of the other three groups (P<0.008).The levels of IL-1, TNF-αand PGE-2 in the material group and brushing group were significantly lower than that of the control group (P<0.008). Compared with materials group, the brushing group had significantly lower level of IL-1,significantly higher level of PGE-2 ,but no difference in the level of TNF-α.In addition, the teeth calculus index of composite group was significantly lower than that of other groups (P<0.05). CONCLUSIONS: CMC-Zn+-P can effectively reduce periodontal tissue inflammation and cut down the speed of deposition of dental calculus. If used cooperatively with brushing, the effect will be better.

Key words: Miniature pigs, Carboxymethyl chitosan zinc and peptide, IL-1, TNF-α, PGE-2

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