局部应用Fe2+、F- 对酸蚀釉质作用的体外研究

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (2): 168-171.

局部应用Fe2+、F- 对酸蚀釉质作用的体外研究

张月^1*，2，种燕³，黄瑞哲^1*,2

1. 西安交通大学口腔医学院陕西省颅颌面精准医学研究重点实验，陕西省牙颌面疾病临床研究中心袁陕西西安710004
2. 西安交通大学口腔医院预防科袁陕西西安710004
3. 西安市第二医院口腔科袁陕西西安710003

收稿日期:2015-03-18 修回日期:2015-03-18 出版日期:2016-03-25 发布日期:2016-05-06
作者简介:张月(1986-),女,硕士,住院医师,E-mail:zhangyue198717@sina.com
基金资助:
西安市卫生局科技项目(J2013008)

Effect of topical iron, fluoride application on eroded enamel in vitro

Yue¹,², CHONG Yan³, HUANG Ruizhe^1,2

1.Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University. Xi'an 710004; 2.Department of Preventive Dentistry, School of Stomatology, Xi'an Jiaotong University. Xi'an 710004;
3.Department of Stomatology, Second Hospital of Xi'an City. Xi'an 710003, Shaanxi Province,China)

Received:2015-03-18 Revised:2015-03-18 Online:2016-03-25 Published:2016-05-06

摘要/Abstract

摘要： 目的:评价硫酸亚铁、氟化钠溶液及两者共同作用时对酸蚀釉质的作用。方法:收集正畸拔除的新鲜完好前磨牙,制备80个样本,随机分为4组:C组(对照组,去离子水)、Fe2+组(15 mmol/L FeSO4)、F-组(1.23% NaF)、Fe2+ + F-组(15 mmol/L FeSO4 + 1.23% NaF)。样本在各组实验溶液中浸泡1 min,然后交替进行6个脱矿-再矿化循环(共7 d)。1个完整周期包括:① 在5 mL可口可乐(pH=2.58)中脱矿5 min;②在37℃、5 mL人工唾液中再矿化1 h。酸蚀效果通过观察釉质表面形貌和测定表面显微硬度(SMH)进行评价,采用SPSS18.0软件包对数据进行单因素方差分析。结果:扫描电镜观察显示,铁、氟处理后,与C组相比,釉质表面粗糙程度显著减轻,各实验组釉质表面均有一定沉积物形成。碳酸饮料浸泡釉质后SMH明显下降。与C组相比,铁、氟制剂单独或联合处理釉质后SMH减少程度均显著降低(P<0.001),但3个实验组间无显著差异。结论:铁、氟制剂单独或联合作用均可显著增强釉质抗酸蚀能力,但铁、氟联合并未出现叠加保护效果。

关键词: 硫酸亚铁, 牙釉质, 酸蚀, 表面形貌, 表面显微硬度

Abstract: PURPOSE: To evaluate the effect of supplementation containing iron and/or fluoride on eroded enamel in vitro. METHODS: Eighty enamel specimens collected in orthodontic treatments were randomly allocated into 4 groups of 20 samples each: C (control,deionized water); Fe2+ (15 mmol/L FeSO4); F- (1.23% NaF) and Fe2+ + F- (15 mmol/L FeSO4 and 1.23% NaF ). Before all samples submitted to 6 alternating demineralization and remineralization cycles, they were pre-treated with one of the experimental solutions for 1 minute. One complete cycle consisted of the following steps: ①demineralization in 5 mL beverage (Coca-Cola, pH=2.58) for 5 minutes under gentle agitation; ②remineralization in 5 mL artificial saliva for 1 hour at 37℃. The effect of erosion was observed by enamel surface morphology and measured by superficial micro-hardness (SMH). One-way ANOVA was performed using SPSS18.0 software package for analysis of the data. RESULTS: The SMH of enamel after erosion by carbonated drinks was significantly lower than the SMH before erosion. When ferrous sulfate with or without fluoride was tested for the prevention of enamel demineralization, there was statistically significant increase in the SMH of enamel in comparison with the control (P＜0.001). However, the experimental solutions did not differ significantly among them. Scanning election microscope showed the deposition on the enamel surface after iron or fluorine treatment ,and the damage degree of the enamel surface were reduced compared with the control. CONCLUSIONS: Iron and/or fluoride was shown to be effective to enhance the acid resistance of enamel,while the combined use of fluoride and iron in group Fe2++F- had no cumulative effects.

Key words: Ferrous sulfate, Enamel, Erosion, Surface morphology, Surface micro-hardness

中图分类号:

R780.1

张月，种燕，黄瑞哲. 局部应用Fe2+、F- 对酸蚀釉质作用的体外研究[J]. 上海口腔医学, 2016, 25(2): 168-171.

Yue,, CHONG Yan, HUANG Ruizhe. Effect of topical iron, fluoride application on eroded enamel in vitro[J]. Shanghai Journal of Stomatology, 2016, 25(2): 168-171.

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