miR-155、miR-146a在口腔扁平苔藓患者外周血和组织中的变化与相关性研究

摘要/Abstract

摘要： 目的探讨miRNA（miR-155、miR-146a及miR-146b）在口腔扁平苔藓（oral lichen planus, OLP）患者外周血及病损组织中的表达及其与OLP临床特征的关系，为进一步探讨OLP的发病机制提供参考。方法采用荧光定量PCR法，以U6基因为内参，分别检测OLP患者外周血及病损组织、健康对照外周血及组织中miR-155、miR-146a及miR-146b的相对含量。采用SPSS17.0软件包对数据进行统计学分析。结果荧光定量PCR检测显示，与正常对照组相比，外周血 miR-155 表达水平在OLP 患者中显著增高，病损组织中升高更加明显（组织中miR-155水平是外周血中的7.0倍）；而OLP患者外周血中miR-146a、miR-146b含量与对照组相比无显著差异（P=0.218，P=0.229），但OLP患者病损组织中miR-146a表达升高，显著高于对照组（P=0.003），且组织中miR-146a水平是外周血中的5倍。相关性分析结果显示，外周血中miR-155的表达水平与REU计分呈高度正相关（相关系数R=0.887，P<0.01）。结论 OLP患者外周血和组织中miR-155、miR-146a异常变化表明，miR-155、miR-146a可能与OLP的发生与演进有关，OLP可能是以局部炎症为主的慢性炎症性疾病。

关键词: 口腔扁平苔藓, miRNA, miR-155, miR-146a

Abstract: PURPOSE To investigate the miRNA (including miR-155, miR-146a and miR-146b) expression in peripheral blood mononuclear cells (PBNCs) or lesions tissues in oral lichen planus (OLP) patients and healthy controls and the relationship between miRNA and OLP clinical features. METHODS The expression of miRNA（miR-155, miR-146a and miR-146b）was assessed by quantitative fluorescent PCR in peripheral blood cells or lesions tissues in OLP patients and healthy controls. U6 was used as reference gene. Statistical analysis was performed with SPSS17.0 software package. RESULTS Real-time PCR showed that the expression of miR-155 in OLP PBNCs was significantly higher than that in controls, miR-155 expression in OLP lesions tissues was 7.0 times higher than that of PBMCs. Correlation analysis showed that the level of miR-155 expression in PBMCs was positively correlated with REU score(correlation coefficient R=0.887, P<0.01). There was no significant difference in miR-146a and miR-146b expression in PBMCs between OLP and control group. On the contrary, the miR-146a expression in OLP lesions tissues was significantly upreguated than that in control group (P=0.003).CONCLUSIONS The results indicated that miR-155 and miR-146a might play important roles in the pathogenesis of OLP and OLP is likely a chronic inflammatory disease characterized by local inflammation.

Key words: Oral lichen planus, miRNA, miR-155, miR-146a

中图分类号:

R781.5

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梁雪艺，胡靖宇，周刚. miR-155、miR-146a在口腔扁平苔藓患者外周血和组织中的变化与相关性研究[J]. 上海口腔医学, 2015, 24(6): 729-734.

LIANG Xue-yi, HU Jing-yu, ZHOU Gang.. Detection of miR-155, miR-146a in PBNCs and tissues from patients with oral lichen planus[J]. Shanghai Journal of Stomatology, 2015, 24(6): 729-734.

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miR-155、miR-146a在口腔扁平苔藓患者外周血和组织中的变化与相关性研究

Detection of miR-155, miR-146a in PBNCs and tissues from patients with oral lichen planus

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[1]	赵湘, 葛化冰. 口腔扁平苔藓患者血清中γ干扰素、白细胞介素4表达水平对免疫应答的调节作用[J]. 上海口腔医学, 2023, 32(3): 271-275.
[2]	杨成龙, 杨蒙, 王宇峰, 宋晨成, 杜观环, 唐国瑶. 具核梭杆菌来源的外膜囊泡对口腔上皮细胞闭合蛋白Claudin-4表达的影响[J]. 上海口腔医学, 2023, 32(2): 126-131.
[3]	王灏钰, 沈雪敏, 沈征宇. 口腔扁平苔藓组织环状RNA表达谱的筛选与初步探讨[J]. 上海口腔医学, 2022, 31(5): 535-539.
[4]	杜洪亮, 何等旗, 王佳俐, 车银富, 闫庆涵. 姜黄素调控唾液腺肿瘤细胞增殖和凋亡的机制探讨[J]. 上海口腔医学, 2022, 31(5): 483-490.
[5]	李晶晶, 宋晨成, 李晨曦, 夏荣辉, 邓一文, 王宇峰, 唐国瑶. 口腔苔藓样损害中淋巴滤泡样结构表征及免疫细胞组成的实验研究[J]. 上海口腔医学, 2021, 30(2): 113-119.
[6]	刘伟东, 赵民. 加味逍遥丸治疗口腔扁平苔藓伴焦虑抑郁状态患者的疗效评价[J]. 上海口腔医学, 2019, 28(3): 312-316.
[7]	高颖，郝晨笛. 慢性牙周炎患者唾液中miR-146a的表达及其与龈沟炎症、MMP-8/TIMP-1水平的关系[J]. 上海口腔医学, 2018, 27(3): 309-312.
[8]	包丽荣, 赵文青, 林田, 陆彦玲, 吴煜. BMP-2诱导人牙髓细胞分化过程中miRNA表达谱的变化[J]. 上海口腔医学, 2017, 26(5): 476-483.
[9]	王翠杰, 李言君, 薛江南, 慈浩粟, 李丽萍, 李玲. 调节性T细胞和白介素15在口腔扁平苔藓中的表达及意义[J]. 上海口腔医学, 2016, 25(4): 438-442.
[10]	王玉, 靳赢, 王奕月, 胥丹妮, 林晓萍. IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析[J]. 上海口腔医学, 2016, 25(4): 443-447.
[11]	胡爱平, 刘宗响. Nd:YAG激光联合白芍总苷胶囊治疗糜烂型口腔扁平苔藓的疗效观察[J]. 上海口腔医学, 2016, 25(4): 481-483.
[12]	黄吉燕, 李含卿, 陈梁, 赵厚明, 周海文. 309例口腔扁平苔藓患者药物依从性与饮食依从性调查分析[J]. 上海口腔医学, 2016, 25(3): 340-344.
[13]	李相如, 郭嘉, 李新明. 口腔扁平苔藓细胞凋亡及凋亡蛋白Bcl-2、Bax表达的变化[J]. 上海口腔医学, 2015, 24(4): 465-469.
[14]	谢三祥, 丰琳, 朱声荣, 丁蕾. 转录因子RORγτ和FOXP3在口腔扁平苔藓病损组织中的表达及意义[J]. 上海口腔医学, 2014, 23(4): 472-476.
[15]	桑涛, 许韩美, 杨艳杰, 马杰. A型人格行为类型与口腔扁平苔藓的相关性分析[J]. 上海口腔医学, 2014, 23(1): 91-94.