富血小板纤维蛋白对体外培养的成骨细胞生物学特性的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (1): 61-64.

富血小板纤维蛋白对体外培养的成骨细胞生物学特性的影响

孙晓琳,周延民,赵静辉,郑玲,杨婷婷

吉林大学口腔医院口腔种植科,吉林长春 130021

收稿日期:2014-06-20 出版日期:2015-02-20 发布日期:2015-07-24
通讯作者: 周延民,E-mail:zhouym62@126.com
作者简介:孙晓琳（1986-）,女,硕士,医师,E-mail:sxl2673366@126.com

The effect of platelet-rich fibrin on biologic characteristics of osteoblasts

SUN Xiao-lin,ZHOU Yan-min,ZHAO Jing-hui,ZHENG Ling,YANG Ting-ting

Department of Oral implantation, Jilin University Affiliated Stomatology Hospital. Changchun 130021, Jilin Province, China

Received:2014-06-20 Online:2015-02-20 Published:2015-07-24

摘要/Abstract

摘要： 目的探讨富血小板纤维蛋白（platelet-rich fibrin, PRF）对成骨细胞生物学特性的影响。方法体外培养成骨细胞MG63,实验组采用PRF,对照组为正常高糖DMEM培养液。以MTT法检测细胞增殖,碱性磷酸酶（ALP）染色法检测ALP活性,免疫组织化学法检测Ⅰ型胶原、骨保护素(OPG)及其配体(RANKL)的表达。采用SPSS17.0软件包对数据进行统计学分析。结果PRF能够促进细胞增殖、ALP分泌、Ⅰ型胶原和骨保护素的表达,对骨保护素配体的表达影响不明显。结论PRF能够促进成骨细胞增殖、分化及骨保护素的表达,PRF可能通过对骨保护素及其配体的调节,参与骨的重建过程。

关键词: 富血小板纤维蛋白, 成骨细胞, 骨保护素及其配体

Abstract: PURPOSE: To investigate the effect of platelet-rich fibrin on biologic characteristics of osteoblasts. METHODS: Osteoblasts were cultured with or without a PRF membrane. The cell proliferation was detected by MTT. The alkaline phosphatase (ALP) stain and the positive expression of collagen typeⅠ, osteoprotegerin and RANKL were measured. The data was statistically analyzed with SPSS17.0 software package. RESULTS: PRF promoted cell proliferation and increased expression of ALP, collagen typeⅠand OPG significantly, but there was no significant impact on expression of RANKL. CONCLUSIONS: PRF promotes osteoblasts proliferation, differentiation and OPG expression. PRF is involved in bone remodeling via regulating expression of OPG and RANKL.

Key words: Platelet-rich fibrin, Osteoblasts, Osteoprotegerin and RANKL

中图分类号:

R780.2

孙晓琳,周延民,赵静辉,郑玲,杨婷婷. 富血小板纤维蛋白对体外培养的成骨细胞生物学特性的影响[J]. 上海口腔医学, 2015, 24(1): 61-64.

SUN Xiao-lin,ZHOU Yan-min,ZHAO Jing-hui,ZHENG Ling,YANG Ting-ting. The effect of platelet-rich fibrin on biologic characteristics of osteoblasts[J]. Shanghai Journal of Stomatology, 2015, 24(1): 61-64.

参考文献

[1] 罗湘杭, 廖二元, 周后德, 等. 人成骨肉瘤MG-63细胞分化特性及分化过程中的基因表达[J]. 湖南医科大学学报,2001,26(2): 107-110.
[2] Sporn MB, Roberts AB, Wakefield LM, et al. Transforming growth factor-beta: biological function and chemical structure [J]. Science, 1986, 233(4763): 532-534.
[3] Schmidt MB, Chen EH, Lynch SE. A review of the effects of insulin-like growth factor and platelet derived growth factor on in vivo cartilage healing and repair [J]. Osteoarthritis Cartilage, 2006, 14(5): 403-412.
[4] Holderfield MT, Hughes CC. Crosstalk between vascular endothelial growth factor, notch, and transforming growth factor-beta in vascular morphogenesis [J]. Circ Res, 2008, 102(6): 637-652.
[5] Assoian RK, Sporn MB. Type beta transforming growth factor in human platelets: release during platelet degranulation and action on vascular smooth muscle cells [J]. J Cell Biol, 1986, 102(4): 1217-1223.
[6] Okuda K, Kawase T, Momose M, et al. Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-β and modulates the proliferation of periodontally related cells in vitro [J]. J Periodontol, 2003, 74(6): 849-857.
[7] Kawase T, Okuda K, Wolff LF, et al. Platelet-rich plasma-derived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteblastic cells in vitro [J]. J Periodontol, 2003, 74(6): 858-864.
[8] 葛琳华, 张伟国, 束蓉. 生长因子在骨改建和牙周骨修复中的作用[J]. 上海口腔医学, 2002, 11(1): 63-65.
[9] 孙浩, 张剑明, 李彦秋. 富血小板纤维蛋白生物特性的研究 [D].天津: 天津医科大学, 2010.
[10] Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF):a second-generation platelet concentrate. Part Ⅰ: technological concepts and evolution [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2006, 101(3): e37-44.
[11] Lundquist R, Dziegiel MH, Agren MS. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin [J]. Wound Repair Regen, 2008, 16(3): 356-363.
[12] He L, Lin Y, Hu X, et al. A comparative study of platelet rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2009, 108(5): 707-713.
[13] Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: Leucocyte activation: a new feature for platelet concentrates? [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod,2006,101(3): e51-55.
[14] Dohan Ehrenfest DM, de Peppo GM, Doglioli P, et al. Slow release of growth factors and thrombospondin-1 in Choukroun’s platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies [J]. Growth Factors, 2009, 27(1): 63-69.
[15] 刘侠, 乐以伦. 碱性磷酸酶与钙化 [J]. 中国生物医学工程学报, 1997, 16(l): 70-88.
[16] Wada T, Nakashima T, Hiroshi N, et al. RANKL-RANK signaling in osteoclastogenesis and bone disease [J]. Trends Mol Med, 2006, 12(1): 17-25.