减阻牵张快速牙移动中TGF-β1表达变化对牙周组织改建的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (5): 513-518.

• 基础研究 • 下一篇

减阻牵张快速牙移动中TGF-β1表达变化对牙周组织改建的影响

李宁¹, 杨乐乐², 彭早霞³, 李佩², 李美静², 陈曦², 徐然²

1.烟台市口腔医院正畸科,山东烟台 264000; 2.西安交通大学医学院第一附属医院口腔科,陕西西安 710061; 3.新疆医科大学第一附属医院口腔科,新疆乌鲁木齐 830000

收稿日期:2013-11-21 修回日期:2014-03-10 出版日期:2014-10-20 发布日期:2015-02-04
通讯作者: 陈曦,E-mail:chen168_xi@163.com
作者简介:李宁(1986-),男,硕士,住院医师,E-mail:yt_lining@163.com
基金资助:
国家自然科学基金(81070863); 陕西省科技攻关项目（2014k11-03-08-02）

Effect of TGF-β1 expression on periodontal tissue reconstruction for rapid teeth movement through reducing resistance and distraction

LI Ning¹, YANG Le-le², PENG Zao-xia³, LI Pei², LI Mei-jing², CHEN Xi², XU Ran²

1.Department of Orthodontics, Yantai Stomatological Hospital. Yantai 264000, Shandong Province; 2.Department of Stomatology, the First Affiliated Hospital, Medical School of Xi’an Jiaotong University. Xi’an 710061, Shaanxi Province; 3.Department of Stomatology, the First Teaching Hospital of Xinjiang Medical University. Wulumuqi 830000,Xinjiang Urgur Autonomous Region, China

Received:2013-11-21 Revised:2014-03-10 Online:2014-10-20 Published:2015-02-04
Supported by:
; Supported by National Natural Scinece Foundation of China (81070863) and Science and Technology Project of Shaanxi Province （2014k11-03-08-02）

摘要/Abstract

摘要： 目的探讨减阻牵张快速牙移动中转化生长因子β1（TGF-β1）在牙周组织改建中的作用。方法 20 只 Beagle 犬随机均分为加力 5、10、15 d、加力 15 d 保持 10、90 d 5组。于下颌骨两侧随机分别采用减阻牵张（实验组）和常规正畸方法（对照组）移动第一前磨牙。各组犬分别于预定时间测量牙移动距离并获取第一前磨牙牙周组织块,进行 H-E、苦味酸-天狼星红、免疫组化染色观察。采用SPSS 18.0软件包对数据进行统计学分析。结果实验组牙移动及牙周改建速度显著快于对照组;2组TGF-β1阳性表达分布区域相似,均于15 d骨生成最活跃阶段达到峰值,但加力各阶段实验组阳性表达显著高于对照组（P<0.05）。结论与常规正畸方法相比,减阻牵张能明显增加移动牙张力侧牙周组织TGF-β1的表达,加速牙周组织改建。

关键词: 牵张成骨, 快速牙移动, 转化生长因子β, 1

Abstract: PURPOSE: To investigate the role of transforming growth factor-β1 (TGF-β1) in periodontal tissue reconstruction during rapid teeth movement through reducing resistance and distraction. METHODS: Twenty Beagle dogs were randomly divided into five groups according to time points: distraction for 5 days, 10 days, 15 days, retaining 10 days after distraction for 15 days, and retaining 90 days after distraction for 15 days. The mandibular first premolar was moved by using reducing resistance and distraction (experimental group) or conventional distraction (control group) randomly. For each group, tooth movement distance was measured and first premolar periodontal tissue pieces were obtained in scheduled time. Hematoxylin-eosin stain, Picric acid-Sirius red, and immunohistochemistry were performed. The data was analyzed by using SPSS 18.0 software package. RESULTS: The speed of tooth movement and periodontal reconstruction of the experimental group were significantly faster than the control group. TGF-β1 positive expression in two groups had similar distribution area, and reached peak at most active stage of osteogenesis in both groups. But in different stages of distraction, the positive expression in the experimental group was significantly higher than the control group(P<0.05). CONCLUSIONS: Compared with conventional orthodontic method, reducing resistance and distraction can obviously increase the expression of TGF-β1 of tension side of the distracted tooth, and accelerate periodontal tissue reconstruction.

Key words: Distraction osteogenesis, Rapid tooth movement, Transforming growth factor- β1

李宁, 杨乐乐, 彭早霞, 李佩, 李美静, 陈曦, 徐然. 减阻牵张快速牙移动中TGF-β1表达变化对牙周组织改建的影响[J]. 上海口腔医学, 2014, 23(5): 513-518.

LI Ning, YANG Le-le, PENG Zao-xia, LI Pei, LI Mei-jing, CHEN Xi, XU Ran. Effect of TGF-β1 expression on periodontal tissue reconstruction for rapid teeth movement through reducing resistance and distraction[J]. Shanghai Journal of Stomatology, 2014, 23(5): 513-518.

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减阻牵张快速牙移动中TGF-β1表达变化对牙周组织改建的影响

Effect of TGF-β1 expression on periodontal tissue reconstruction for rapid teeth movement through reducing resistance and distraction

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