低氧对牙周膜成纤维细胞增殖和成骨分化的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (4): 397-401.

低氧对牙周膜成纤维细胞增殖和成骨分化的影响

董家辰^{1, 2}, 宋忠臣^{1, 2}, 束蓉^{1, 2}, 李松^{1, 2}, 林智恺^{1, 2}, 张秀丽²

1.上海交通大学医学院附属第九人民医院·口腔医学院牙周病科;
2.上海市口腔医学研究所,上海市口腔医学重点实验室,上海 200011

收稿日期:2013-11-29 出版日期:2014-08-20 发布日期:2014-10-20
通讯作者: 束蓉,Tel: 021-23271699-5510,E-mail:shurong123@hotmail.com
作者简介:董家辰(1987-), 男, 硕士研究生, E-mail:dongjiachensky@163.com
基金资助:
国家自然科学基金(81070838,81271156); 上海交通大学医工交叉研究基金(YG2011MS31)

The effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells

DONG Jia-chen^1,2, SONG Zhong-chen^1,2, SHU Rong^1,2, LI Song^1,2, LIN Zhi-kai^1,2,ZHANG Xiu-li²

1.Department of Periodontology, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;
2.Shanghai Research Institute of Stomatology, Shanghai Key Lab of Stomatology. Shanghai 200011, China

Received:2013-11-29 Online:2014-08-20 Published:2014-10-20
Supported by:
Supported by National Natural Science Foundation of China(81070838,81271156) and Biomedical Engineering Cross Research Foundation of Shanghai Jiao Tong University(YG2011MS31).

摘要/Abstract

摘要： 目的:观察低氧对体外培养的人牙周膜成纤维细胞(periodontal ligament cells, PDLCs)增殖与成骨分化的影响。方法:体外培养鉴定人牙周膜成纤维细胞,分别用浓度为0、100、200、400 μmol/L的CoCl2作用于细胞,采用MTT法观察CoCl2对PDLCs增殖的影响,运用实时定量PCR和Western免疫印迹技术检测CoCl2模拟低氧对PDLCs成骨分化的影响。采用SPSS13.0软件包对数据进行统计学分析。结果:免疫组化染色结果证实,培养细胞为牙周膜成纤维细胞。200 μmol/L及400 μmol/L的CoCl2均能抑制PDLCs的增殖及碱性磷酸酶、RUNX2、Ⅰ型胶原的表达,并呈剂量依赖性。结论:氯化钴模拟的低氧环境对PDLCs增殖及成骨分化具有抑制作用。

关键词: 牙周膜成纤维细胞, 低氧, 氯化钴, 成骨分化

Abstract: PURPOSE: To investigate the effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells (PDLCs) in vitro. METHODS: Human PDLCs were isolated and characterized. The proliferation rate of PDLCs under different concentration of CoCl2 were tested by MTT assay. The PDLCs’ osteogenic differentiation were investigated using real-time PCR and Western blot. The date was statistically analyzed with SPSS13.0 software package. RESULTS: Immunocytochemical staining verified that the isolated cells were PDLCs. The proliferation of PDLCs and the expression of alkaline phosphatase (ALP), RUNX2, collagen I were significantly decreased in a dose-dependent manner by 200 μmol/L CoCl2 and 400μmol/L CoCl2. CONCLUSIONS: Hypoxia inhibits proliferation and osteogenic differentiation of human PDLCs.

Key words: Periodontal ligament cells, Hypoxia, Cobalt chloride, Osteogenic differentiation

中图分类号:

R781.4

董家辰, 宋忠臣, 束蓉, 李松, 林智恺, 张秀丽. 低氧对牙周膜成纤维细胞增殖和成骨分化的影响[J]. 上海口腔医学, 2014, 23(4): 397-401.

DONG Jia-chen, SONG Zhong-chen, SHU Rong, LI Song, LIN Zhi-kai,ZHANG Xiu-li. The effect of hypoxia on proliferation and osteogenic differentiation of periodontal ligament cells[J]. Shanghai Journal of Stomatology, 2014, 23(4): 397-401.

参考文献

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