低氧条件下人牙源性干细胞促血管相关miRNAs的表达差异研究

doi:10.19439/j.sjos.2021.06.001

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (6): 561-566.doi: 10.19439/j.sjos.2021.06.001

低氧条件下人牙源性干细胞促血管相关miRNAs的表达差异研究

林薪海, 王昊东, 江龙

上海交通大学医学院附属第九人民医院口腔综合科,上海交通大学口腔医学院,国家口腔医学中心, 国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室, 上海 200011

收稿日期:2021-03-15 修回日期:2021-04-28 发布日期:2022-03-09
通讯作者: 江龙,E-mail：jianglong25a@163.com
作者简介:林薪海（1995-）,男,硕士研究生,E-mail：linxinhaibaby@163.com
基金资助:
国家自然科学基金（81300867）

Differential expression of pro-angiogenic miRNAs in dental tissue-derived stem cells under hypoxia

LIN Xin-hai, WANG Hao-dong, JIANG Long

Department of General Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai 200011, China

Received:2021-03-15 Revised:2021-04-28 Published:2022-03-09

摘要/Abstract

摘要： 目的: 探讨人根尖乳头干细胞（stem cells from the apical papilla,SCAPs）、牙髓干细胞（dental pulp stem cells,DPSCs）和牙周膜干细胞（periodontal ligament stem cells, PDLSCs）在低氧条件下促血管相关微小RNA (miRNAs) 的表达及其意义。方法: 从离体牙中提取SCAPs、DPSCs 和PDLSCs,分别于常氧和低氧条件下培养1、3、5 d,通过实时定量PCR(RT-PCR) 检测细胞中已被证实具有特异性促血管相关功能的9个miRNAs的表达变化,以及低氧诱导因子1α (HIF-1α) 的表达变化。采用SPSS 19.0软件包对数据进行统计学分析。结果: 低氧条件下第5天,SCAPs中miR-126的表达相对升高,miR-20a、miR-20b、miR-21、miR-130a、miR-132、miR-210、miR-503的表达相对降低;DPSCs中miR-21、miR-130a、miR-126和miR-210的表达相对升高,miR-132的表达相对降低;PDLSCs中miR-126、miR-210和miR-296的表达相对升高。SCAPs、DPSCs和PDLSCs中HIF-1α的表达相对升高。结论: 低氧条件下,SCAPs、DPSCs和PDLSCs中促血管相关miRNAs具有各自特征性的表达谱。

关键词: 牙源性干细胞, 微小RNA, 低氧, 表达谱

Abstract: PURPOSE: To explore pro-angiogenic miRNAs differentially expressed in dental tissue-derived stem cells under hypoxia. METHODS: Stem cells from apical papilla, dental pulp stem cells and periodontal ligament stem cells were obtained from 3 patients, and treated with nomoxia or hypoxia for 1, 3, 5 d, respectively. Real-time PCR was performed to evaluate the expression of 9 specifically identified pro-angiogenic miRNAs and HIF-1α. SPSS 19.0 software package was used for statistical analysis. RESULTS: At the fifth day of hypoxic condition, as for SCAPs, the expression of miR-126 was relatively increased while miR-20a, miR-20b, miR-21, miR-130a, miR-132, miR-210 and miR-503 deceased. As for DPSCs, the expression of miR-21, miR-130a, miR-126 and miR-210 were relatively increased while miR-132 decreased. As for PDLSCs, the expression of miR-126, miR-21 and miR-296 were relatively increased. Finally, the expression of HIF-1α was relatively upregulated in all three type of cells. CONCLUSIONS: Pro-angiogenic miRNAs in dental tissue-derived stem cells under hypoxia exists specific expression profile.

Key words: Dental tissue-derived stem cells, microRNA, Hypoxia, Expression profile

中图分类号:

R781.3

林薪海, 王昊东, 江龙. 低氧条件下人牙源性干细胞促血管相关miRNAs的表达差异研究[J]. 上海口腔医学, 2021, 30(6): 561-566.

LIN Xin-hai, WANG Hao-dong, JIANG Long. Differential expression of pro-angiogenic miRNAs in dental tissue-derived stem cells under hypoxia[J]. Shanghai Journal of Stomatology, 2021, 30(6): 561-566.

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低氧条件下人牙源性干细胞促血管相关miRNAs的表达差异研究

Differential expression of pro-angiogenic miRNAs in dental tissue-derived stem cells under hypoxia

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