CPP-ACP与不同浓度氟促进釉质再矿化的研究

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (5): 533-536.

CPP-ACP与不同浓度氟促进釉质再矿化的研究

刘路，杨林，邹敏

(西安交通大学口腔医院正畸科，陕西西安 710004)

收稿日期:2013-01-29 修回日期:2013-04-22 出版日期:2013-10-10 发布日期:2013-10-10
通讯作者: 邹敏,Tel:029-87218243,E-mail：zoumin@mail.xjtu.edu.cn
作者简介:刘路(1985-)，女，硕士研究生，住院医师，Tel：029-87218243， E-mail：doctorliulu@126.com

Remineralization of enamel promoted by casein phosphopeptide-amorphous calcium phosphate with different concentration of fluorin

LIU Lu, YANG Lin, ZOU Min

Department of Orthodontics, Xi’an Jiaotong University. Xi’an 710004, Shaanxi Province, China

Received:2013-01-29 Revised:2013-04-22 Online:2013-10-10 Published:2013-10-10

摘要/Abstract

摘要： 目的：探讨酪蛋白磷酸多肽-无定形磷酸钙(casein phosphopeptide-amorphous calcium phosphate，CPP-ACP) 与不同浓度氟离子联合应用对脱矿釉质再矿化的作用。方法：取正畸拔除的健康前磨牙30颗，乳酸凝胶法制备人工早期釉质脱矿后, 随机分为对照组(5% CPP-ACP组)、实验组1(5% CPP-ACP+500 mg/L F-组)和实验组2(5% CPP-ACP+900 mg/L F-组)，采用MH-5型显微硬度仪测量处理前、后釉质标本的显微硬度，采用SPSS 18.0软件包对数据进行统计学分析。结果：显微硬度测量显示，实验组2牙釉质的表面显微硬度变化显著高于实验组1(P<0.05)及对照组(P<0.01)。结论：CPP-ACP能够促进脱矿釉质再矿化，氟对其有加强协同作用。在一定范围内，氟浓度越高，协同作用越好。

关键词: 酪蛋白磷酸多肽-无定形磷酸钙, 氟, 再矿化, 显微硬度

Abstract: PURPOSE: To evaluate the effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) combined with different concentrations of fluoride on demineralization of enamel remineralization. METHODS: Extracted premolar teeth for orthodontic reason were immersed into lactic acid gel to prepare artificial white spot lesions. Then the specimens were randomly assigned to 3 groups: 5% CPP-ACP group as control, 5% CPP-ACP +500 mg/L F- group and 5% CPP-ACP +900 mg/L F- group, which were measured by micro-hardness tester. SPSS 18.0 software package was used for data analysis. RESULTS: There were significant differences in micro-hardness between control group and experimental group 1 and experimental group 2 by SNK-q test (P<0.05). CONCLUSIONS: CPP-ACP can make the demineralization of enamel remineralization occurs. F can promote the CPP-ACP remineralization. The increase of fluoride can make the CPP-ACP remineralization better below 1000-1500 mg/L.

Key words: APP-ACP, Florid, Enamel remineralization, Micro-hardness

中图分类号:

R783.5

刘路, 杨林, 邹敏. CPP-ACP与不同浓度氟促进釉质再矿化的研究[J]. 上海口腔医学, 2013, 22(5): 533-536.

LIU Lu, YANG Lin, ZOU Min. Remineralization of enamel promoted by casein phosphopeptide-amorphous calcium phosphate with different concentration of fluorin[J]. Shanghai Journal of Stomatology, 2013, 22(5): 533-536.

参考文献

[1] Artun J, Brobakken BO. Prevalence of carious white spots after orthodontic treatment with multibonded appliances[J]. Eur J Orthod, 1986, 8(4): 229-234.
[2] Mizrahi E. Enamel demineralization following orthodontic treatment[J]. Am J Orthod, 1982, 82(1): 62-67.
[3] Gorelick L, Geiger AM, Gwinnett AJ. Incidence of white spot formation after bonding and banding[J]. Am J Orthod,1982,81(2): 93-98.
[4] 胡炜, 王勤, 傅民魁, 等. 口腔正畸固定矫治器应用中牙釉质脱矿的临床调查[J]. 口腔正畸学, 2001, 8 (2): 51-54.
[5] 周春华, 孙新华, 黄洋, 等. CPP-ACP与氟促进脱矿釉质再矿
化的显微放射定量分析[J]. 吉林大学学报(医学版),2008,37(6): 1022-1026.
[6] Reynolds EC. Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions[J]. J Dent Res, 1997, 76 (9): 1587-1595.
[7] Huysmans MC, Longbottom C. The challenges of validating diagnostic methods and selecting appropriate gold standards[J]. J Dent Res, 2004, 83(Spec No C): C48-52.
[8] Reynolds EC, Cai F, Cochrane NJ, et al. Fluoride and casein phosphopeptide-amorphous calcium phosphate[J]. J Dent Res, 2008, 87(4): 344-348.
[9] Cross KJ, Huq NL, Palamara JE, et al. Physicochemical characterization of casein phosphopeptide-amorphous calcium phosphate nanocomplexes[J]. J Biol Chem, 2005, 280(15): 15362-15369.
[10] Srinivasan N, Kavitha M, Loganathan SC. Comparison of the remineralization potential of CPP -ACP and CPP-ACP with 900 ppm fluoride on eroded human enamel: an in situ study[J]. Arch Oral Biol, 2010, 55(7): 541-544.
[11] Linton JL. Quantitative measurements of remineralization of incipient caries[J]. Am J Orthod Dentofacial Orthop,1996,110(6): 590-597.
[12] Damato FA, Strang R, Stephen KW. Effect of fluoride concentration on remineralization of carious enamel: an in vitro pH-cycling study[J]. Caries Res, 1990, 24(3): 174-180.
[13] Cross KJ, Huq NL, Stanton DP, et al. NMR studies of a novel calcium, phosphate and fluoride delivery vehicle-alpha (S1)-casein (59-79) by stabilized amorphous calcium fluoride phosphate nanocomplexes[J]. Biomaterials, 2004, 25(20): 5061-5069.

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