抗菌肽rHBD3对义齿软衬材料表面白色念珠菌黏附性能的影响

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (4): 389-392.

抗菌肽rHBD3对义齿软衬材料表面白色念珠菌黏附性能的影响

石勇¹，宋玮²，刘晓芳¹，高利敏¹，宋光保¹

(1.广东省口腔医院修复科，广东广州 510280；2.广州军区联勤部门诊部，广东广州 510080)

收稿日期:2013-01-08 修回日期:2013-03-04 出版日期:2013-08-10 发布日期:2013-08-10
通讯作者: 石勇，Tel：020-84427049，E-mail:83516422@qq.com
作者简介:石勇(1978-)，男，博士研究生，副主任医师
基金资助:
广东省医学科学技术研究科研基金(B2012038)

Use of recombinant human beta-defensin-3 to evaluate the effect of adhesion of Candida albicans on the surface of soft lining material

SHI Yong¹, SONG Wei², LIU Xiao-fang¹, GAO Li-min¹, SONG Guang-bao¹

1.Department of Prosthodontics, Guangdong Provincial Stomatological Hospital, Affiliated Hospital of Southern Medical University. Guangzhou 510280; 2. Department of Outpatient Service, Affiliated to the Logistics Department of Guangzhou Military Command. Guangzhou 510080, Guangdong Province, China

Received:2013-01-08 Revised:2013-03-04 Online:2013-08-10 Published:2013-08-10
Supported by:
Supported by Medical Science and Technology Research Fund of Guangdong Province (B2012038).

摘要/Abstract

摘要： 目的：初步评价重组人β防御素3(rHBD3)对附着于义齿软衬硅橡胶材料表面白色念珠菌的抑菌效果。方法：在软衬硅橡胶试件上面接种0.1 mL的白色念珠菌，然后将试件分别浸泡在25 μg/mL rHBD3和5.25%NaClO溶液中5、10、30、60 min。将接种过菌液的一面的试件放置在BHI琼脂培养板上15 s。隔天计算菌落数，采用SPSS10.0软件包对数据进行双因素方差分析。利用激光共聚焦显微镜观察rHBD3对白色念珠菌形成的细菌生物膜的作用。结果：白色念珠菌菌落数在rHBD3作用不同时间后，变化有显著差异(P<0.05)。30 min后，rHBD3与5.25%NaClO组间无显著差异。白色念珠菌生物膜在rHBD3作用30 min后， SYTO9/PI染色显示死菌的数量明显增多。结论：rHBD3不仅能够消除软衬表面白色念珠菌等微生物的感染，而且对其形成的细菌生物膜也有很好的抑菌效果。rHBD3用于软衬材料的清洁维护，为预防义齿性口炎的发生提供了实验依据。

关键词: 人β防御素3, 抑菌性能, 白色念珠菌, 软衬材料

Abstract: PURPOSE: To evaluate the effectiveness of recombinant human beta-defensin-3 (rHBD3) on reduction of Candida albicans from the surface of soft lining materials. METHODS: Specimens made of soft lining materials, which had been contaminated with C. albicans, were immersed in 25 μg/mL rHBD3 and 5.25% sodium hypochlorite (a positive control) for 5, 10, 30 and 60 min. The results were analyzed statistically with SPSS 10.0 software package. Confocal laser scanning microscopy(CLSM) of biofilm-associated C. albicans were treated with rHBD3 in different times. RESULTS: The results confirmed that an immersion time of 30 min or higher yielded a good disinfection effect, both for the experimental group and the positive control. A large number of pathogenic bacteria were killed and the cells were stained red by SYTO9/PI after 30 min. CONCLUSIONS: It is possible that soft lining materials immersed in rHBD3 solution that possesses antifungal activity may be clinically protective.

Key words: Human beta-defensin 3, Antimicrobial activity, C. albicans, Soft lining materials

中图分类号:

R783.6

石勇, 宋玮, 刘晓芳, 高利敏, 宋光保. 抗菌肽rHBD3对义齿软衬材料表面白色念珠菌黏附性能的影响[J]. 上海口腔医学, 2013, 22(4): 389-392.

SHI Yong, SONG Wei, LIU Xiao-fang, GAO Li-min, SONG Guang-bao. Use of recombinant human beta-defensin-3 to evaluate the effect of adhesion of Candida albicans on the surface of soft lining material[J]. Shanghai Journal of Stomatology, 2013, 22(4): 389-392.

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