储存条件对离体牛牙釉质粘接剪切强度的影响

doi:10.19439/j.sjos.2023.02.006

上海口腔医学 ›› 2023, Vol. 32 ›› Issue (2): 143-146.doi: 10.19439/j.sjos.2023.02.006

储存条件对离体牛牙釉质粘接剪切强度的影响

李莉¹, 谭军艳¹, 陈文彬², 汪隼^3,*, 王玉波^2,*

1.青岛大学附属青岛市口腔医院儿童口腔科,山东青岛 266001;
2.青岛大学附属青岛市口腔医院口腔修复科,山东青岛 266001;
3.上海交通大学医学院附属第九人民医院儿童口腔科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2021-07-22 修回日期:2021-11-16 出版日期:2023-04-25 发布日期:2023-06-13
通讯作者: 汪隼,E-mail：wangsun9h@163.com;王玉波,E-mail：myshaya@126.com。^*共同通信作者
作者简介:李莉（1979-）,女,硕士研究生,E-mail：lilyeyesonyou@163.com
基金资助:
青岛市医疗卫生重点学科建设项目; 青岛市口腔疾病临床医学研究中心（22-3-7-lczx-7-nsh）

Effect of various storage methods on shear bond strength of enamel of bovine teeth

LI Li¹, TAN Jun-yan¹, CHEN Wen-bin², WANG Sun³, WANG Yu-bo²

1. Department of Pediatric Dentistry, Qingdao Stomatological Hospital Affiliated to Qingdao University. Qingdao 266001, Shandong Province;
2. Department of Prosthodontics, Qingdao Stomatological Hospital Affiliated to Qingdao University. Qingdao 266001, Shandong Province;
3. Department of Pediatric Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2021-07-22 Revised:2021-11-16 Online:2023-04-25 Published:2023-06-13

摘要/Abstract

摘要： 目的: 比较不同储存条件下牛牙的釉质粘接剪切强度,探讨适合储存离体牛牙的条件。方法: 选择新鲜拔除的牛下前牙130颗,随机分为13组,每组10颗;其中,1组为对照组,12组为实验组。参照组在牛牙获取当天开始进行实验。实验组储存条件如下：4%甲醛溶液（4 ℃、30天,23 ℃、30天,4 ℃、90天,23 ℃、90天）、1%氯胺-T溶液（4 ℃、30天,23 ℃、30天,4 ℃、90天,23 ℃、90天）、蒸馏水（4 ℃、30天,23 ℃、30天,4 ℃、90天,23 ℃、90天）。储存完成后,将树脂粘接于牛牙釉质表面,测定其粘接剪切强度。采用SPSS 20.0软件包对测试结果进行统计学分析。结果: 在23 ℃储存条件下,4%甲醛溶液和1%氯胺-T溶液储存30天和90天,粘接强度与新鲜牙釉质无显著差异。在4 ℃储存条件下,蒸馏水溶液储存30天和90天,粘接强度与新鲜牙釉质无显著差异。在4 ℃储存条件下,4%甲醛溶液和1%氯胺-T溶液储存30天,釉质粘接强度高于新鲜牛牙;90天时,与新鲜牛牙釉质粘接强度无显著差异。在23 ℃储存条件下,蒸馏水储存30 天时粘接强度与新鲜牛牙釉质无显著差异;但到90天时,釉质粘接强度低于新鲜牛牙。结论: 用4%甲醛溶液、1%氯胺-T溶液在23 ℃储存和蒸馏水4 ℃储存,粘接强度与新鲜牙釉质无显著差异,不随时间变化而变化。建议使用4%甲醛溶液和1%氯胺-T溶液在23 ℃、蒸馏水在4 ℃储存离体牛牙。

关键词: 牛牙, 牙釉质, 剪切强度, 储存条件

Abstract: PURPOSE: The purpose of this study was to evaluate the effect of various storage methods on shear bond strength of enamel of bovine teeth and find the storage condition that could preserve the similar bond strength as the freshly extracted teeth. METHODS: One hundred and thirty freshly extracted bovine teeth were divided into 13 groups. One was the reference group and 12 were the experimental group. Each group contained 10 teeth. Teeth in the reference group were operated on the same day as the teeth were extracted, while teeth in the experimental groups were stored in different methods (4% formaldehyde solution at 4 ℃, 23 ℃, 1% chloramine T at 4 ℃, 23 ℃, distilled water at 4 ℃, 23 ℃). After stored for 30 days and 90 days, the bovine teeth were taken out and then the shear bond strength was tested. The data were analyzed with SPSS 20.0 software package. RESULTS: The bovine teeth stored in 4% formaldehyde and 1% chloramine T at 23 ℃ and in distilled water at 4 ℃ achieved similar bond strength as freshly extracted teeth at 30 days and 90 days, and the bond strength did not change over time. The bovine teeth stored in 4% formaldehyde solution and 1% chloramine T at 4 ℃ at 30 days had higher shear bond strength than freshly extracted bovine teeth, but over time the bond strength reduced and reached the similar level at 90 days. The bovine teeth stored in distilled water at 23 ℃ obtained similar bond strength as freshly extracted teeth at 30 days but over time the bond strength reduced until 90 days. CONCLUSIONS: Bovine teeth stored in 4% formaldehyde solution and 1% chloramine T at 23 ℃ and in distilled water at 4 ℃ achieved similar bond strength as freshly extracted teeth and does not change over time. These three methods are recommended for storing bovine teeth.

Key words: Bovine teeth, Enamel, Shear bond strength, Storage method

中图分类号:

R783.1

李莉, 谭军艳, 陈文彬, 汪隼, 王玉波. 储存条件对离体牛牙釉质粘接剪切强度的影响[J]. 上海口腔医学, 2023, 32(2): 143-146.

LI Li, TAN Jun-yan, CHEN Wen-bin, WANG Sun, WANG Yu-bo. Effect of various storage methods on shear bond strength of enamel of bovine teeth[J]. Shanghai Journal of Stomatology, 2023, 32(2): 143-146.

参考文献

[1] Attin T, Wegehaupt F, Gries D, et al.The potential of deciduous and permanent bovine enamel as substitute for deciduous and permanent human enamel: erosion-abrasion experiments[J]. J Dent, 2007, 35(10): 773-777.
[2] 楚金普, 周学东. 人牙与牛牙用于人工龋损实验的比较病理学观察[J]. 实验动物与比较医学, 2014, 34(3): 176-180.
[3] ISO TC 11405-2003, Dental materials-Guidance on testing of adhesion to tooth structure[S].
[4] Fonseca RB, Haiter-Neto F, Fernandes-Neto AJ, et al.Radiodensity of enamel and dentin of human, bovine and swine teeth[J]. Arch Oral Biol, 2004, 49(11): 919-922.
[5] Oesterle LJ, Shellhart WC, Belanger GK.The use of bovine enamel in bonding studies[J]. Am J Orthod Dentofacial Orthop, 1998, 114(5): 514-519.
[6] Nakamichi I, Iwaku M, Fusayama T.Bovine teeth as possible substitutes in the adhesion test[J]. J Dent Res, 1983, 62(10): 1076-1081.
[7] Anjum A, Otsuki M, Matin K, et al.Preservation in the liquid media produces alterations in enamel surface properties[J]. J Dent, 2009, 37(11): 884-890.
[8] Jameson MW, Tidmarsh BG, Hood JA.Effect of storage media on subsequent water loss and regain by human and bovine dentine and on mechanical properties of human dentine in vitro[J]. Arch Oral Biol, 1994, 39(9): 759-767.
[9] Titley KC, Chernecky R, Rossouw PE, et al.The effect of various storage methods and media on shear-bond strengths of dental composite resin to bovine dentine[J]. Arch Oral Biol, 1998, 43(4): 305-311.
[10] Da Silva EM, Glir DH, Gill AW, et al.Effect of chlorhexidine on dentin bond strength of two adhesive systems after storage in different media[J]. Braz Dent J, 2015, 26(6): 642-647.
[11] Ziskind D, Gleitman J, Rotstein I, et al.Evaluation of cetylpyridinium chloride for infection control in storage solution[J]. J Oral Rehabil, 2003, 30(5): 477-481.
[12] Aquilino SA, Williams VD, Svare CW.The effect of storage solutions and mounting media on the bond strengths of a dentinal adhesive to dentin[J]. Dent Mater, 1987, 3(3): 131-135.
[13] DeWald JP. The use of extracted teeth for in vitro bonding studies: a review of infection control considerations[J]. Dent Mater, 1997, 13(2): 74-81.
[14] Della Bona A, van Noort R. Shear vs. tensile bond strength of resin composite bonded to ceramic[J]. J Dent Res, 1995, 74(9): 1591-1596.
[15] Cardoso PE, Braga RR, Carrilho MR.Evaluation of micro-tensile, shear and tensile tests determining the bond strength of three adhesive systems[J]. Dent Mater, 1998, 14(6): 394-398.
[16] Triolo PT Jr, Swift EJ Jr.Shear bond strengths of ten dentin adhesive systems[J]. Dent Mater, 1992, 8(6): 370-374.
[17] Haller B, Hofmann N, Klaiber B, et al.Effect of storage media on microleakage of five dentin bonding agents[J]. Dent Mater, 1993, 9(3): 191-197.
[18] Söderholm KJ.Correlation of in vivo and in vitro performance of adhesive restorative materials: a report of the ASC MD156 task group on test methods for the adhesion of restorative materials[J]. Dent Mater, 1991, 7(2): 74-83.
[19] Raum K, Kempf K, Hein HJ, et al.Preservation of microelastic properties of dentin and tooth enamel in vitro-a scanning acoustic microscopy study[J]. Dent Mater, 2007, 23(10): 1221-1228.
[20] Al-Salehi SK, Wood DJ, Hatton PV.The effect of 24 h non-stop hydrogen peroxide concentration on bovine enamel and dentine mineral content and microhardness[J]. J Dent, 2007, 35(11): 845-850.

储存条件对离体牛牙釉质粘接剪切强度的影响

Effect of various storage methods on shear bond strength of enamel of bovine teeth

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	谢惠兰, 陈江, 曹白雨, 林毅. 负载氯己定的埃洛石纳米管对牙本质粘结强度的影响[J]. 上海口腔医学, 2022, 31(5): 471-475.
[2]	陈月, 鄢国伟, 郑月梅, 周凯璇, 谢翠柳. 碳二亚胺联合不同粘接技术对牙本质粘接性能的影响[J]. 上海口腔医学, 2022, 31(1): 48-53.
[3]	万里扬, 朱洪水. 热碱溶液蚀刻对氧化锆与树脂粘接强度的影响[J]. 上海口腔医学, 2017, 26(6): 590-593.
[4]	王思钱，张大风，郑铁丽，杨静远，林婷婷，麻健丰. 纳米SiO2溶胶对氧化锆基底与饰面瓷结合强度的影响[J]. 上海口腔医学, 2016, 25(2): 141-145.
[5]	张月，种燕，黄瑞哲. 局部应用Fe2+、F- 对酸蚀釉质作用的体外研究[J]. 上海口腔医学, 2016, 25(2): 168-171.
[6]	董俊超，温黎明，李任，李金源. 不同处理剂对氧化锆与正畸托槽间黏结强度的影响[J]. 上海口腔医学, 2016, 25(2): 177-180.
[7]	张月,黄瑞哲,. Fe2+、F-对釉质抗酸性的增强作用及机制[J]. 上海口腔医学, 2016, 25(1): 42-46.
[8]	苑克勇,马瑞,朱彩莲,李鸣宇,何智妍,周薇. 冷光美白对釉质表面细菌生物膜形成的影响[J]. 上海口腔医学, 2015, 24(3): 283-287.
[9]	吴海苗,赵丙姣,陈栋. 不同处理方法对托槽再黏结强度的影响[J]. 上海口腔医学, 2015, 24(3): 307-310.
[10]	程竑, 乔广艳, 董聪, 张富强. 不同表面处理对氧化锆与牙釉质黏结强度的影响[J]. 上海口腔医学, 2014, 23(4): 436-440.
[11]	钟群, 彭艳, 邬雪颖, 翁佳炜. 不同表面处理方式对Kavo氧化锆基底与饰瓷结合强度的影响[J]. 上海口腔医学, 2014, 23(3): 300-303.
[12]	徐强, 张文茹, 孟贺, 王琳. 氟保护漆抑制酸性含乳饮料对乳牙釉质脱矿的作用[J]. 上海口腔医学, 2014, 23(1): 51-57.
[13]	徐丹, 项楠, 魏斌,. 金属修复体表面粗糙度对其磨耗性能的影响[J]. 上海口腔医学, 2013, 22(3): 270-273.
[14]	赵蒙蒙, 王青山, 王爽, 王方辉, 舒静媛. 新型纳米羟基磷灰石复合树脂与牙本质的黏结性能评价[J]. 上海口腔医学, 2013, 22(3): 274-277.
[15]	王晓鸣;徐侃;傅远飞. 不同形状固位钉对前牙基托抗剪切力的影响 [J]. 上海口腔医学, 2012, 21(6): 648-650.