上海口腔医学

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (2): 208-212.doi: 10.19439/j.sjos.2020.02.018

• 论著 • 上一篇    下一篇

种植体周围炎与健康种植体黏膜下微生态环境的对照研究

赵湘, 葛化冰, 冯洁, 杜岩   

  1. 首都医科大学附属北京口腔医院王府井部 口腔综合科,北京 100006
  • 收稿日期:2019-12-03 修回日期:2020-01-08 出版日期:2020-04-25 发布日期:2020-04-30
  • 通讯作者: 葛化冰,E-mail:ghb7009@aliyun.com
  • 作者简介:赵湘(1975-),女,本科,主治医师,E-mail:snowfox155@163.com

Comparative study of submucosal microbial ecological environment between human peri-implantitis and healthy implants

ZHAO Xiang, GE Hua-bing, FENG Jie, DU Yan   

  1. Department of General Dentistry, Wangfujing Branch of Beijing Stomatological Hospital, Capital Medical University. Beijing 100006, China
  • Received:2019-12-03 Revised:2020-01-08 Online:2020-04-25 Published:2020-04-30

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摘要： 目的 探讨种植体周围炎与健康种植体黏膜下微生物生态环境。方法 选取2017年9月—2019年1月于北京口腔医院治疗种植体周围炎、接受种植术并已随访1年以上的患者,按照是否发生种植体周围炎分为种植体周围炎组(n=43,51颗种植体)与健康组(n=59,63颗种植体)。检测2组患者探诊深度、骨吸收情况、探诊出血情况、改良菌斑指数(modified plaque index,mPLI)、改良出血指数(modified bleeding index,mSBI)。收集2组患者黏膜下菌斑,使用微生物基因提取试剂盒提取细菌DNA,经Illumina公司的MiSeq测序平台测序。采用SPSS 23.0软件包对数据进行统计学分析。结果 种植体周围炎组平均最大探诊深度显著大于健康组(P<0.05);mPLI 2级[56.86%(29/51)]显著高于健康组[0.00%(0/63)](P<0.05);mSBI 2级[60.78%(31/51)]显著高于健康组[0.00%(0/63)](P<0.05)。种植体周围炎组Simpson指数显著高于健康组,Shannon指数显著低于健康组(P<0.05)。种植体周围炎组优势菌属中占比最多的为新月形单胞菌属(16.35%)、依次为假单胞菌属(11.20%)、梭杆菌属(10.05%)、链球菌属(7.63%)、密螺旋体菌属(6.84%)。结论 与健康种植体相比,种植体周围炎黏膜下微生物具有高物种丰度、低物种均匀度,其优势菌属中的假单胞菌属、新月形单胞菌属、梭杆菌属、链球菌属、密螺旋体菌属占比较高。

关键词: 种植体周围炎, 探诊深度, 微生物, 生态平衡

Abstract: PURPOSE: To investigate the balance of submucosal microbial ecological environment in human peri-implantitis and healthy implants. METHODS: The patients with peri-implantitis and dental implants in Beijing Stomatological Hospital, and followed up for one year from September 2017 to January 2019 were enrolled. Patients were divided into two groups according to the presence or absence of peri-implantitis, i.e. peri-implantitis group (43, 51 implants) and healthy group (59 patients, 63 implants). The probing depth, bone resorption, probing bleeding, mPLI and mSBI were recorded. DNA of the submucosal microflora was extracted using a microbial gene extraction kit and sequenced by Illumina's MiSeq sequencing platform. Logistic regression analysis was used to analyze the correlation between the dominant bacteria and the average maximum probing depth, mPLI grading and MSBI grading in peri-implantitis group. The data were processed by SPSS 23.0 software package. RESULTS: The average maximum probing depth of peri-implantitis group was significantly higher than that in healthy group (P<0.05). The proportion of mPLI grade 2 in peri-implantitis group was significantly higher than that in healthy group [56.86%(29/51) vs 0.00%(0/63), P<0.05]. The proportion of mSBI grade 2 in peri-implantitis group was significantly higher than that in healthy group [60.78%(31/51) vs 0.00%(0/63), P<0.05]. The Simpson index of peri-implantitis group was significantly higher than that of the healthy group, and the Shannon index was significantly lower than the healthy group (P<0.05). In peri-implantitis group, the dominant microflora were Selenomonas (16.35%), Pseudomonas (11.20%), Fusobacterium (10.05%), Streptococcus (7.63%) and Treponema (6.84%). CONCLUSIONS: Compared with healthy implants, submucosal microorganisms of peri-implantitis have high species abundance and low species evenness, Pseudomonas, Selenomonas, Fusobacterium, Streptococcus and Treponema account for high proportions in the dominant bacteria.

Key words: Peri-implantitis, Probing depth, Microbes, Ecological balance

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