低温常压等离子体对金黄地鼠颊囊黏膜溃疡组织中SOD活性、MDA含量的影响

doi:10.19439/j.sjos.2017.04.005

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (4): 374-378.doi: 10.19439/j.sjos.2017.04.005

低温常压等离子体对金黄地鼠颊囊黏膜溃疡组织中SOD活性、MDA含量的影响

郑丽, 陈铭晟, 王璐

重庆医科大学口腔医学院,口腔疾病与生物医学重庆市重点实验室,重庆市高校市级口腔生物医学工程重点实验室,重庆 401147

收稿日期:2017-02-04 修回日期:2017-03-21 出版日期:2017-08-25 发布日期:2017-09-01
通讯作者: 陈铭晟,E-mail：masonchenpublic@126.com
作者简介:郑丽(1991-),女,在读硕士研究生,E-mail：644383133@qq.com
基金资助:
国家自然科学基金（81400570）

Effect of a non-thermal atmospheric pressure argon plasma jet on SOD and MDA levels in oral mucosal ulcer of golden hamsters

ZHENG Li, CHEN Ming-sheng, WANG Lu

College of Stomatology, Chongqing Medical University, Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education. Chongqing 401147, China

Received:2017-02-04 Revised:2017-03-21 Online:2017-08-25 Published:2017-09-01

摘要/Abstract

摘要： 目的探讨低温常压等离子体对金黄地鼠实验性颊囊黏膜溃疡愈合时间及溃疡组织中超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量的影响。方法56只金黄地鼠随机分为正常对照组、溃疡模型组、自然愈合组、药物组、2个通气组（30 s、120 s组）及2个等离体组（30 s、120 s组）。末次处理结束24 h后,每组随机选取4只动物,测定溃疡组织中SOD、MDA水平,观察剩余动物溃疡愈合时间。采用SPSS 22.0软件包对数据进行统计学分析。结果等离子体组溃疡愈合时间较其余各组显著缩短（P=0.00）。末次处理结束24 h后,等离子体组SOD活性、MDA含量与正常组相比,差异无显著性（P>0.05）,其SOD活性与其余各组相比显著升高（P=0.00）、MDA含量与其余各组相比则显著降低（P=0.00）。30 s、120 s等离子体组SOD、MDA水平无显著差异（P>0.05）。结论一定剂量的等离子体照射能有效升高溃疡组织中SOD活性、降低MDA含量,有效缩短口腔溃疡愈合时间。

关键词: 等离子体, 口腔溃疡, 超氧化物歧化酶, 丙二醛, 金黄地鼠

Abstract: To observe the effects of a non-thermal atmospheric pressure argon plasma jet on the healing time, and the levels of superoxide dismutase（SOD）and malondialdehyde（MDA）in oral mucosal ulcer of golden hamsters．METHODS： 56 golden hamsters were randomly assigned to 8 groups, one normal control group, one ulcer group, one natural healing group without any therapy, one iodine glycerin group, two argon groups (30 s, 120 s)and two plasma groups (30 s, 120 s)．24 hours after the last treatment, 4 hamsters from each group were sacrificed to detect SOD and MDA levels in the oral mucosal ulcer by spectrophotometry. The healing time in the remaining hamsters was observed continuously for 9 days after the last treatment．The data were analyzed with SPSS 22.0 software package. RESULTS：The healing time of the plasma groups was significantly shorter than the other groups (P=0.00). 24 h after the last treatment, there was no significant difference between the plasma groups and the normal contro1 group in SOD and MDA levels (P>0.05), SOD level in plasma groups was significantly higher than other groups (P=0.00), and MDA level in plasma groups was significantly lower than other groups (P=0.00). There was no significant difference between 30 s and 120 s plasma group in SOD and MDA levels (P>0.05). CONCLUSIONS: Non-thermal atmospheric pressure plasma in certain dose can increase SOD level and decrease MDA level in ulcer tissues effectively, and reduce the healing time of oral ulcer in golden hamsters．

Key words: Plasma, Oral ulcer, Superoxide dismutase, Malondialdehyde, Golden hamster

中图分类号:

R781.5

郑丽, 陈铭晟, 王璐. 低温常压等离子体对金黄地鼠颊囊黏膜溃疡组织中SOD活性、MDA含量的影响[J]. 上海口腔医学, 2017, 26(4): 374-378.

ZHENG Li, CHEN Ming-sheng, WANG Lu. Effect of a non-thermal atmospheric pressure argon plasma jet on SOD and MDA levels in oral mucosal ulcer of golden hamsters[J]. Shanghai Journal of Stomatology, 2017, 26(4): 374-378.

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低温常压等离子体对金黄地鼠颊囊黏膜溃疡组织中SOD活性、MDA含量的影响

Effect of a non-thermal atmospheric pressure argon plasma jet on SOD and MDA levels in oral mucosal ulcer of golden hamsters

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