人β防御素在牙髓组织中的表达及与炎症因子的关系

doi:10.19439/j.sjos.2017.04.004

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (4): 368-373.doi: 10.19439/j.sjos.2017.04.004

人β防御素在牙髓组织中的表达及与炎症因子的关系

翟越¹, 黄枧英², 朴玄¹, 纪芳³, 费照亮⁴, 陶疆¹

1．上海交通大学医学院附属第九人民医院·口腔医学院口腔综合科,上海市口腔医学重点实验室,上海市口腔医学研究所,国家口腔疾病临床研究中心,上海 200011；
2.江西省妇幼保健院新生儿疾病筛查中心中心实验室, 江西南昌 330002；
3.上海交通大学医学院附属第九人民医院·口腔医学院口腔正畸科,上海市口腔医学重点实验室, 上海市口腔医学研究所,国家口腔疾病临床研究中心,上海 200011；
4.中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海 200031

收稿日期:2017-02-15 修回日期:2017-04-06 出版日期:2017-08-25 发布日期:2017-09-01
通讯作者: 陶疆, E-mail: taojiang_doctor@hotmail.com
作者简介:翟越（1991-）,女,硕士,E-mail:zhaiyuekouqiang@yeah.net
基金资助:
国家自然科学基金（81200756、81500813）; 上海高校高峰高原学科建设项目

Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue

ZHAI Yue¹, HUANG Jian-ying², HYUN Park¹, JI Fang³, FEI Zhao-liang⁴, TAO Jiang¹

1.Department of General Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011;
2.Central Laboratory, Center for Neonatal Disease Screening, Maternal and Child Health Hospital of Jiangxi Province. Nanchang 330002, Jiangxi Province;
3.Department of Orthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011;
4.Institute of Biochemistry and Cell Biology,Shanghai Institute for Biological Sciences,Chinese Academy of Sciences.Shanghai 200031, China

Received:2017-02-15 Revised:2017-04-06 Online:2017-08-25 Published:2017-09-01

摘要/Abstract

摘要： 目的探讨人β防御素（human β-defensin, HBD）家族成员在人牙髓组织中的表达,以HBD2为代表,探讨HBD在牙髓炎症反应中所受调控及HBD家族成员之间的调节关系。方法在NCBI GEO Profiles数据库中检索HBD在人牙髓组织中表达的基因芯片信息,并运用反转录聚合酶链式反应（RT-PCR）进行验证。运用4种炎症因子TNF-α、IL-1α、IL-1β和IL-6不同组合刺激人牙髓细胞,采用实时荧光定量核酸扩增检测法（qPCR）检测相应组分对HBD2表达水平的影响;HBD110预处理及LPS刺激牙髓细胞后,采用qPCR方法检测TNF-α、IL-1α和HBD2的表达水平。采用GraphPad Prism 5.01 对实验组和对照组的结果进行统计学分析。结果在NCBI GEO Profiles数据库中检索到27种HBD家族成员在人牙髓组织中表达。TNF-α、IL-1α、IL-1β和IL-6联合作用,可显著提升HBD2的表达水平。HBD110通过调控TNF-α和IL-1α的表达,提高HBD2的表达水平。结论除已有报道的HBD1、HBD2、HBD3和HBD4外,其他HBD家族成员在人牙髓组织中表达,且HBD2在牙髓组织中受炎症因子及其他HBD家族成员的调控。

关键词: 人β, 防御素, 牙髓, 炎症反应

Abstract: To investigate the expression of human β-defensin(HBD) in human dental pulp tissue and to explore the regulation of HBD in pulp inflammation and the relationship among HBD family members. METHODS: The gene expression of HBD in human dental pulp tissue was assessed in NCBI GEO profiles and was verified by RT-PCR. Human dental pulp cells were stimulated with TNF-α, IL-1α, IL-1β and IL-6 in different combinations and the expression of HBD2 was analyzed by qPCR. Human dental pulp cells were pretreated with HBD110 and then stimulated with LPS and the expression of TNF-α,IL-1α and HBD2 were analyzed by qPCR. GraphPad Prism 5.01 was used to analyze the results of the experimental and the control groups. RESULTS: 27 HBDs were found to express in human dental pulp tissue in NCBI GEO Profiles. The joint overexpression of TNF-α, IL-1α, IL-1β and IL-6 increased the expression of HBD2; HBD110 increased the expression of HBD2 by increasing the expression of TNF-α and IL-1α. CONCLUSIONS: Many other HBDs have positive expression in human dental pulp issue besides of HBD1, HBD2, HBD3, HBD4 and the inflammation factors and other HBDs can regulate the expression of HBD2 in dental pulp.

Key words: Human β, -defensin, Dental pulp, Inflammation

中图分类号:

R781.3

翟越, 黄枧英, 朴玄, 纪芳, 费照亮, 陶疆. 人β防御素在牙髓组织中的表达及与炎症因子的关系[J]. 上海口腔医学, 2017, 26(4): 368-373.

ZHAI Yue, HUANG Jian-ying, HYUN Park, JI Fang, FEI Zhao-liang, TAO Jiang. Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue[J]. Shanghai Journal of Stomatology, 2017, 26(4): 368-373.

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人β防御素在牙髓组织中的表达及与炎症因子的关系

Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue

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