Expression of IL-34 in chronic periapical lesions and its clinical significance

Shanghai Journal of Stomatology ›› 2016, Vol. 25 ›› Issue (1): 53-57.

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Expression of IL-34 in chronic periapical lesions and its clinical significance

MA Nan, QU Liu, XU Li-ya, YU Ya-qiong, QIU Li-hong.

Department of Endodontics, School of Stomatology, China Medical University;
Lab of Endodontics, Liaoning Institute of Dental Research;
Research Center of Translational Medicine, Liaoning Provincial Institute of Oral Diseases. Shenyang 110002, Liaoning Province, China

Received:2015-07-13 Revised:2015-09-18 Online:2016-02-25 Published:2016-03-09

Abstract

Abstract: PURPOSE: To observe the expression of IL-34 mRNA in chronic periapical lesions and healthy periodontal ligaments and discuss the role of IL-34 in the etiology of chronic apical periodontitis. METHODS： A total of 25 periapical tissues from chronic apical periodontitis and 22 normal periodontal ligament tissue from extracted healthy teeth for orthodontic reason were selected. The expression of IL-34mRNA was detected by real-time PCR; the expression of IL-34 protein was detected by immunohistochemical analysis. Statistical analysis was performed using SPSS 13.0 software package. RESULTS: The level of IL-34 mRNA expression in periapical lesions (3.53±3.07) was significantly higher than that of the normal control (1.07±0.76); IL-34 was positively expressed in lymphocytes, plasma cells and macrophages. Image analysis software indicated that the level of IL-34 protein was significantly higher in periapical lesions than that in normal control (P<0.01). CONCLUSIONS: IL-34 may be closely related to inflammation of chronic apical periodontitis.

Key words: IL-34, Chronic apical periodontitis, RT-PCR, Immunohistochemstry

CLC Number:

R781.34

MA Nan, QU Liu, XU Li-ya, YU Ya-qiong, QIU Li-hong.. Expression of IL-34 in chronic periapical lesions and its clinical significance[J]. Shanghai Journal of Stomatology, 2016, 25(1): 53-57.

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Expression of IL-34 in chronic periapical lesions and its clinical significance

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