Expression of SOCS-1 and SOCS-3 in chronic periapical lesions and its clinical significance

doi:10.19439/j.sjos.2017.04.007

Abstract

Abstract: To detect the expression of suppressor of cytokine signaling-1 (SOCS-1) and suppressor of cytokine signaling-3 (SOCS-3) in chronic periapical lesions and to clarify their roles in pathogenesis of apical periodontitis. METHODS: A total of 25 chronic periapical lesion tissues and 16 normal periodontal ligament tissues were collected respectively. The expression of mRNA was measured by real-time PCR, the protein expression was measured by immunohistochemical analysis. Statistical analysis was performed using SPSS 13.0 software package. RESULTS: Immunohistochemical results indicated that expression levels of SOCS-1 and SOCS-3 protein in chronic periapical lesions were significantly higher than that in normal tissues (P<0.01); Moreover, SOCS-1 and SOCS-3 protein expression levels in severe inflammation group were significantly higher than that in mild inflammation group (P<0.01). In mild inflammation group, severe inflammation group and control group, the expression levels of SOCS-1 mRNA were 2.620±1.552, 2.373±1.083 and 1.277±1.040, whereas those of SOCS-3 were 9.308±5.901, 7.565±3.233 and 1.232±1.099, respectively. CONCLUSIONS: The expression levels of SOCS-1 mRNA and SOCS-3 mRNA are significantly higher both in mild inflammation group and severe inflammation group than that in control group (P<0.01), while no significant difference is found in mild and severe inflammation group.

Key words: SOCS-1, SOCS-3, Real-time PCR, Chronic apical periodontitis

CLC Number:

R781.3

WANG Si-mo, MA Nan, QIU Li-hong, LI Xiao-lin, YANG Di, XUE Ming. Expression of SOCS-1 and SOCS-3 in chronic periapical lesions and its clinical significance[J]. Shanghai Journal of Stomatology, 2017, 26(4): 384-388.

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