Expression and clinical significance of plasma microRNA-125b level in patients with oral squamous cell carcinoma

Abstract

Abstract: PURPOSE: To explore the expression and clinical significance of plasma microRNA-125b (miR-125b) in patients with oral squamous cell carcinoma (OSCC). METHODS: By Stem-loop RT real-time PCR based on the SYBR Green, we investigated the expression of plasma miR-125b in 85 OSCC patients and 46 healthy controls, then evaluated its diagnostic performance as OSCC biomarker. Data analysis was performed with SPSS 17.0 software package for one-way ANOVA and receiver operating characteristic (ROC) curve. RESULTS: Stem-loop RT-PCR could specifically amplify miR-125b in plasma. The expression of plasma miR-125b was significantly increased in patients with OSCC compared with healthy controls (P<0.05). ROC results showed that miR-125b could differentiate OSCC from healthy controls(AUC=0.966). As fold change 3.46 was chosen for optimal cutoff value, the diagnostic sensitivity and specificity was 89.4% and 93.5%, respectively. CONCLUSIONS: Due to the satisfactory diagnostic performance, plasma miR-125b can be used as a promising biomarker in OSCC.

Key words: Oral squamous cell carcinoma, miR-125b, Plasma detection, Biomarker

CLC Number:

R739.8

GU Wen-li,YE Dong-xia,WU Jing-jing. Expression and clinical significance of plasma microRNA-125b level in patients with oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2015, 24(1): 71-75.

References

[1] Bartel DP. MicroRNAs: target recognition and regulatory functions [J]. Cell, 2009,136(2):215-233.
[2] Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function [J]. Cell, 2004,116(2):281-297.
[3] Esquela-Kerscher A, Slack FJ. Oncomirs - microRNAs with a role in cancer [J]. Nat Rev Cancer, 2006,6(4):259-269.
[4] Jackson RJ, Standart N. How do microRNAs regulate gene expression [J]. Sci STKE, 2007,2007(367):re1.
[5] Jannot G, Simard MJ. Tumour-related microRNAs functions in Caenorhabditis elegans [J]. Oncogene, 2006,25(46):6197-6201.
[6] Lu J, Getz G, Miska EA, et al. MicroRNA expression profiles classify human cancers [J]. Nature, 2005,435(7043):834-838.
[7] Cortez MA, Calin GA. MicroRNA identification in plasma and serum: a new tool to diagnose and monitor diseases[J]. Expert Opin Biol Ther, 2009,9(6):703-711.
[8] Park NJ, Zhou H, Elashoff D, et al. Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection [J]. Clin Cancer Res, 2009,15(17): 5473-5477.
[9] Hanke M, Hocham D, Warnecke JM, et al. A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer [J]. Urol Oncol, 2010,28(6):655-661.
[10] Valadi H, Ekstrom K, Bossios A, et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells [J]. Nat Cell Biol, 2007,9(6):654-659.
[11] Zheng D, Haddadin S, Wang Y, et al. Plasma microRNAs as novel biomarkers for early detection of lung cancer[J]. Int J Clin Exp Pathol, 2011,4(6):575-586.
[12] Croce CM. Causes and consequences of microRNA dysregulation in cancer [J]. Nat Rev Genet, 2009,10(10):704-714.
[13] Park NJ, Zhou H, Elashoff D, et al. Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection [J]. Clin Cancer Res, 2009,15(17): 5473-5477.
[14] Mitchell PS, Parkin RK, Kroh EM, et al. Circulating microRNAs as stable blood-based markers for cancer detection [J]. Proc Natl Acad Sci USA, 2008, 105(30):10513-10518.
[15] Taylor DD, Gercel-Taylor C. MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer [J]. Gynecol Oncol, 2008,110(1):13-21.
[16] Sempere LF, Freemantle S, Pitha-Rowe I, et al. Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation [J]. Genome Biol, 2004,5(3):R13.
[17] Lee YS, Kim HK, Chung S, et al. Depletion of human micro-RNA miR-125b reveals that it is critical for the proliferation of differentiated cells but not for the down-regulation of putative targets during differentiation [J]. J Biol Chem, 2005, 280(17):16635-16641.
[18] Schaefer A, Jung M, Mollenkopf HJ, et al. Diagnostic and prognostic implications of microRNA profiling in prostate carcinoma [J]. Int J Cancer, 2010,126(5):1166-1176.
[19] Mattie MD, Benz CC, Bowers J, et al. Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies [J]. Mol Cancer, 2006,5:24.
[20] Wang H, Tan G, Dong L, et al. Circulating MiR-125b as a marker predicting chemoresistance in breast cancer [J]. PLoS One, 2012,7(4):e34210.
[21] Tang F, Zhang R, He Y, et al. MicroRNA-125b induces metastasis by targeting STARD13 in MCF-7 and MDA-MB-231 breast cancer cells [J]. PLoS One, 2012, 7(5):e35435.
[22] Ma Y, Tian Z, Zang W. Circulating miR-125b is a novel biomarker for screening non-small-cell lung cancer and predicts poor prognosis [J]. J Cancer Res Clin Oncol, 2012,138(12):2045-2050.