Study of salivary metabonomics in patients with oral squamous cell carcinoma and leukoplakia

doi:10.19439/j.sjos.2021.02.018

Abstract

Abstract: PURPOSE: The objective of this study was to detect and analyze the existence of differential metabolites from saliva of patients with oral squamous cell carcinoma(OSCC) by metabonomics method, and to evaluate its application value on clinical diagnosis or screening of OSCC. METHODS: The collected saliva samples for OSCC patients, oral leukoplakiac(OLK) patients who were pathologically diagnosed and met the inclusion criteria were analyzed using metabonomics methods (GC-TOF-MS). The results were analyzed by principal component analysis and orthogonal partial least squares discriminant analysis using SIMCA software. RESULTS: The metabolic profiles of OSCC, OLK and healthy control group showed significant differences (P<0.05). In total, 15 typical differential metabolites among the three groups were detected. Further study focusing on metabolic pathway revealed imbalance of protein, energy and lipid metabolism between OSCC and OLK patients. Abnormal tricarboxylic acid cycle was detected as well. CONCLUSIONS: Metabonomics methods is feasible for differential metabolites analysis,15 differential metabolites were detected in OSCC,OLK patients and healthy people. These findings will contribute to the early screening of oral squamous cell carcinoma .

Key words: Oral squamous cell carcinoma, Oral leukoplakia, Metabonomics

CLC Number:

R739.8

XUE Jia-yi, ZHU Xiao-yan, WEI Chang-lei, SUN Pei, ZHANG Hui, DENG Jing. Study of salivary metabonomics in patients with oral squamous cell carcinoma and leukoplakia[J]. Shanghai Journal of Stomatology, 2021, 30(2): 201-205.

References

[1] Amit M, Yen T, Liao C, et al.Improvement in survival of patients with oral cavity squamous cell carcinoma: an international collaborative study[J]. Cancer, 2013,119(24): 4242-4248.
[2] Sakamoto Y, Matsushita Y, Yamada SI, et al.Risk factors of distant metastasis in patients with squamous cell carcinoma of the oral cavity[J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2015, 121(5): 474-480.
[3] Brouns E, Baart J, Bloemena E, et al.The relevance of uniform reporting in oral leukoplakia: definition, certainty factor and staging based on experience with 275 patients[J]. Med Oral Patol Oral Cir Bucal, 2012, 18(1): e19-e26.
[4] Liu Y, Li Y, Fu Y, et al.Quantitative prediction of oral cancer risk in patients with oral leukoplakia[J]. Oncotarget, 2017, 8(28): 46057-46064.
[5] 王宇峰, 尚书, 周曾同, 等. 口腔白斑癌变率与癌变时间及其影响因素的回顾分析[J]. 上海口腔医学, 2011, 20(1): 65-71.
[6] Kim SJ, Kim SH, Kim JH, et al.Understanding metabolomics in biomedical research[J]. Endocrinol Metab, 2015, 31(1): 7-16.
[7] Gupta A, Gupta S, Mahdi A, et al.1H NMR-derived serum metabolomics of leukoplakia and squamous cell carcinoma[J]. Clin Chim Acta, 2015, 441(1): 47-55.
[8] Ohshima M, Sugahara K, Kasahara K, et al.Metabolomic analysis of the saliva of Japanese patients with oral squamous cell carcinoma[J]. Oncol Rep, 2017, 37(5): 2727-2734.
[9] Navazesh M.Methods for collecting saliva[J]. Ann N Y Acad Sci, 1993, 694(1):72-77.
[10] Martínez-Arranz, Ibon, Mayo R, Pérez-Cormenzana M, et al. Enhancing metabolomics research through data mining[J]. J Proteomics, 2015,127(Pt B): 275-288.
[11] Lubes G, Goodarzi M.GC-MS based metabolomics used for the identification of cancer volatile organic compounds as biomarkers[J]. J Pharm Biomed Anal, 2018, 147: 313-322.
[12] Wang Q, Yu Q, Lin Q, et al.Emerging salivary biomarkers by mass spectrometry[J]. Clin Chim Acta, 2015, 438: 214-221.
[13] Koneru S, Tanikonda R.Salivaomics - a promising future in early diagnosis of dental diseases[J]. Dent Res J, 2014, 11(1): 11-15.
[14] Schwartz L, Supuran CT, Alfarouk KO.The warburg effect and the hallmarks of cancer[J]. Anticancer Agents Med Chem, 2017, 17(2): 164-170.
[15] Hui S, Ghergurovich JM, Morscher RJ, et al.Glucose feeds the TCA cycle via circulating lactate[J]. Nature, 2017, 551(7678): 115-118.
[16] Olsson B, Cox TF, Psarelli EE, et al.Relationship between serum concentrations of nitisinone and its effect on homogentisic acid and tyrosine in patients with alkaptonuria[J]. JIMD Rep, 2015, 24(1):21-27.
[17] Hussain SP, Harris CC.Inflammation and cancer: an ancient link with novel potentials[J]. Int J Cancer, 2007, 121(11): 2373-2380.
[18] Sheng H, Shao J, Washington MK, et al.Prostaglandin E2 increases growth and motility of colorectal carcinoma cells[J]. J Biological Chem, 2001, 276(21): 18075-18081.