The effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts

Abstract

Abstract: PURPOSE: To investigate the effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts through a model mimicking the periodontal wound healing and regeneration. METHODS: Human vascular endothelial cells, periodontal ligament cells and gingival fibroblasts were co-cultured using a Transwell system. The number of each type of cells at 2, 4, 6, 8 and 10 days after co-culture were counted using a cell counter machine, while periodontal ligament cells and gingival fibroblasts single culture were used as control. The results were statistically processed with SPSS 13.0 software package. RESULTS: When vascular endothelial cell was present, the proliferation of periodontal ligament cells and gingival fibroblasts were faster than each single culture, the difference between them was significant (P<0.01). The proliferation of periodontal ligament cells was faster than gingival fibroblast as they were co-cultured with vascular endothelial cells(which happened at day 6), although the results were contrary when they were single cultured, the difference between them was significant (P<0.01). CONCLUSIONS: The presence of vascular endothelial cells can promote the proliferation of periodontal cells, and the effect is more prominent on human periodontal ligament cells than on gingival fibroblasts.

Key words: Periodontal regeneration, Vascular endothelial cells, Cell proliferation

CLC Number:

R781.4

DIN Jin, YIN Yuan-zheng. The effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts[J]. Shanghai Journal of Stomatology, 2013, 22(5): 518-522.

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