Quantitative real-time PCR for target periodontal bacteria in subgingival plaque before and after local delivery of periocline, scaling and root planning

Shanghai Journal of Stomatology ›› 2013, Vol. 22 ›› Issue (3): 297-301.

• Clinical Study • Previous Articles Next Articles

Quantitative real-time PCR for target periodontal bacteria in subgingival plaque before and after local delivery of periocline, scaling and root planning

DENG Shu-li¹, WANG Ying¹, HE Jia-yan², CHEN Zhuo¹, CHEN Hui¹

1. Department of Conservative Dentistry, Affiliated Hospital of Stomatology，College of Medicine, Zhejiang University. Hangzhou 310006; 2. Institute of Agriculture and Biological Technology, Zhejiang University. Hangzhou 310003, Zhejiang Province, China

Received:2012-12-13 Revised:2013-01-08 Online:2013-06-10 Published:2013-06-10
Supported by:
Supported by 2011 National Clinical Specialist Construction Project; Natural Science Foundation of Zhejiang Province(LY13H140002); Education Department Funds of Zhejiang Province(20061258) and Medical General Research Project of Zhejiang Province(2012KYB121).

Abstract

Abstract: PURPOSE: To compare the copy number of Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi) in subgingival plaque before and after local delivery of periocline (2% minocycline hydrochloride ointment, MO), scaling and root planning (SRP) by quantitative real-time PCR (qRT-PCR) and evaluate the efficacy. METHODS: Sixty-two adults with moderate to severe chronic periodontitis were selected in the study. Microbial samples were taken from pocket before and after MO and SRP(7d). The samples were evaluated by qRT-PCR for Pg and Pi. Microbiological effectiveness of treatments was assessed using Kruskal-Wallis and Wilcoxon rank-sum test. All tests were two-sided with a significance level of 0.05. All analyses were conducted with SAS 9.1.3 software package. RESULTS: The copy number of Pg and Pi in subgingival plaque was 103-106 and 102-106. Bacterial loads of Pg were reduced in SPR+ MO, SRP and MO site. The counts of Pi decreased in SRP+ MO sites compared with those in the MO or SRP alone sites significantly (P<0.05). CONCLUSIONS: Quantitative real-time PCR (qRT-PCR) is used as a powerful tool with high sensitivity and specificity to quantitatively assess target periodontal bacteria. The results show that subgingival administration of MO and SRP was effective for reducing pathogenic bacteria and improving clinical outcome.

Key words: Quantitative real-time PCR, Porphyromonas gingivalis, Prevotella intermedia

CLC Number:

R781.42

DENG Shu-li, WANG Ying, HE Jia-yan, CHEN Zhuo, CHEN Hui. Quantitative real-time PCR for target periodontal bacteria in subgingival plaque before and after local delivery of periocline, scaling and root planning[J]. Shanghai Journal of Stomatology, 2013, 22(3): 297-301.

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Quantitative real-time PCR for target periodontal bacteria in subgingival plaque before and after local delivery of periocline, scaling and root planning

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