Expression and distribution of SDF-1 in the soft tissue healing of tooth extraction

Abstract

Abstract: PURPOSE: To observe the expression and distribution of stromal cell derived factor -l (SDF-1) in the soft tissues after tooth extraction, in order to provide new ideas to promote wound healing of tooth extraction. METHODS: Thirty male Wistar rats were randomly divided into 10 groups. After extracting the first molar of left mandibular respectively, immunohistochemistry and RT-PCR technique were used to evaluate the distribution and expression of SDF-1 1, 2, 4, 7 and 10 days after extraction. Data processing was performed using SPSS 12.0 software package. RESULTS: Immunohistochemical staining showed the SDF-1 protein was strongly expressed at the gingival tissues around tooth extraction wound at early stage, mainly in the cytoplasm and intercellular substance of the stratum spinosum and stratum basale, and stained more obviously closer to the stratum basale. Four days after tooth extraction, the expression of SDF-1 in the stratum basale became more evident, and it is also positive inside endothelial cells of granulation tissues. Seven days after tooth extraction, the staining became uniform in the gingival epithelium, and a few positive staining of vascular endothelial cells could be found in lamina propria; Ten days after tooth extraction, the staining characteristics were similar to the normal gingiva. RT-PCR results showed that SDF-1mRNA underwent a biphasic expression change during gingival wound healing. SDF-1 mRNA level reached peak at day 1 after tooth extraction (P<0.01) but decreased by day 2. However, the SDF-1 mRNA level increased again to a peak at day 4 and then returned to a normal level by day 10 (P>0.05). CONCLUSIONS: SDF-1 is involved in the early soft tissue healing process, and may play a role as a promoter in tooth extraction healing.

Key words: Stromal cell-derived factor-1, Tooth extraction, Soft tissue healing

CLC Number:

R782.1

LI Lei,CUI Jun,HUO Yuan-yuan,RONG Zhi-cheng,ZHANG Qiang,LI Zhao-yuan. Expression and distribution of SDF-1 in the soft tissue healing of tooth extraction[J]. Shanghai Journal of Stomatology, 2015, 24(3): 269-274.

References

[1] Kassim B, Ivanovski S, Mattheos N. Current perspectives on the role of ridge (socket) preservation procedures in dental implant treatment in the aesthetic zone [J]. Aust Dent J, 2014, 59(1): 48-56.
[2] Döring Y, Pawig L, Weber C, et al. The CXCL12/CXCR4 chemokine ligand/receptor axis in cardiovascular disease [J]. Front Physiol, 2014, 5: 212.
[3] Cui J, Ma XN, Wen Y, et al. Stromal-cell-derived factor 1: potentially an important promoter in healing of tooth extraction or dental implantation to stimulate the host healing mechanism? [J]. Indian J Dent, 2014, 5(1): 28-31.
[4] Proksch S, Steinberg T, Stampf S, et al. Crosstalk on cell behavior in interactive cocultures of hMSCs with various oral cell types [J]. Tissue Eng Part A, 2012, 18(23-24): 2601-2610.
[5] Stellos K, Gawaz M. Platelets and stromal cell-derived factor-1 in progenitor cell recruitment [J]. Semin Thromb Hemost, 2007, 33(2): 159-164.
[6] Dar A, Goichberg P, Shinder V, et al. Chemokine receptor CXCR4-dependent internalization and resecretion of functional chemokine SDF-1 by bone marrow endothelial and stromal cells[J]. Nat Immunol, 2005, 6(10): 1038-1046.
[7] Cao J, Wang L, Du ZJ, et al. A comparison of stromal cell-derived factor-1 expression during distraction osteogenesis and bone fracture in the mandible [J]. J Craniofac Surg, 2013, 24(3): 805-808.
[8] Cheng JW, Sadeghi Z, Levine AD, et al. The role of CXCL12 and CCL7 chemokines in immune regulation, embryonic development, and tissue regeneration [J]. Cytokine, 2014, 69(2): 277-283.
[9] 冯帅南, 朱方强, 王莎莉,等.基质细胞衍生因子-1α在皮肤创伤愈合过程中的表达特点 [J]. 第三军医大学学报, 2009, 31(6): 474-476.
[10] Xu X, Zhu F, Zhang M, et al. Stromal Cell-derived factor-1 enhances wound healing through recruiting bone marrow-derived mesenchymal stem cells to the wound area and promoting neovascularization[J]. Cells Tissues Organs,2013,197(2):103-113.
[11] Yang D, Sun S, Wang Z, et al. Stromal Cell-derived factor-1 receptor CXCR4- overexpressing bone marrow mesenchymal stem cells accelerate wound healing by migrating into skin injury [J]. Cell Reprogram, 2013, 15(3): 206-215.
[12] Ceradini DJ, Kulkarni AR, Callaghan MJ, et al. Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1 [J]. Nat Med, 2004, 10(8): 858-864.
[13] Tepper OM, Capla JM, Galiano RD, et al. Adult vasculogenesis occurs through in situ recruitment, proliferation, and tubulization of circulating bone marrow-derived cells [J]. Blood, 2005, 105(3): 1068-1077.
[14] Gallagher KA, Liu ZJ, Xiao M, et a1. Diabetic impairments in NO-mediated endothelial progenitor cell mobilization and homing are reversed by hyperoxia and SDF-1 alpha [J]. J Clin Invest, 2007, 117(5): 1249-1259.