Changes of α-SMA, type I and type Ⅲ collagen in gingival tissue and expression of MMP-2 and TIMP-2 in gingival crevicular fluid under orthodontic force

doi:10.19439/j.sjos.2021.04.012

Abstract

Abstract: PURPOSE: To investigate the changes of α-smooth muscle actin (α-SMA), type Ⅰ and type Ⅲ collagen in gingival tissue and expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in gingival crevicular fluid under orthodontic force. METHODS: Seventy-four patients undergoing orthodontic treatment in Affiliated Stomatological Hospital of Nanchang University from April 2018 to April 2019 were enrolled, and randomly divided into three groups. Group A(n=24) received the treatment under 0 g of orthodontic force, group B (n=25) under 75 g of orthodontic force, and group C(n=25) under 150 g of orthodontic force. At the baseline and 4th week of treatment, the expression levels of α-SMA, type I collagen and type Ⅲ collagen in gingival tissues were detected by immunohistochemical staining. At the baseline, the 2nd, and 4th week of treatment, the expression levels of MMP-2 and TIMP-2 in gingival crevicular fluid were detected by enzyme-linked immunosorbent assay. Then the correlation between different orthodontic force and levels of α-SMA, type Ⅰ collagen, type Ⅲ collagen, MMP-2 and TIMP-2 was analyzed using Spearman correlation analysis. Statistical analysis was completed by SPSS 19.0 software package. RESULTS: At the 2nd and 4th week of treatment, the expression levels of MMP-2 and TIMP-2 in gingival crevicular fluid among three groups were the lowest in group A, followed by group B and group C(P<0.05). At the 4th week of treatment, the levels of α-SMA, type Ⅰ and type Ⅲ collagen in gingival tissues among three groups were the lowest in group A, followed by group B and group C(P<0.05). The orthodontic force was positively correlated with expression levels of α-SMA, type Ⅰ collagen, type Ⅲ collagen, MMP-2 and TIMP-2(P<0.05). CONCLUSIONS: The expression levels of MMP-2 and TIMP-2 in gingival crevicular fluid and myofibroblast are related to the changes of orthodontic force, which may play an important role in the reconstruction of periodontal tissue during orthodontic treatment.

Key words: Orthodontic force, Gingival crevicular fluid, α-smooth muscle actin, Matrix metalloproteinase

CLC Number:

R783.5

JIANG Li-ping, TANG Zhen. Changes of α-SMA, type I and type Ⅲ collagen in gingival tissue and expression of MMP-2 and TIMP-2 in gingival crevicular fluid under orthodontic force[J]. Shanghai Journal of Stomatology, 2021, 30(4): 402-405.

References

[1] 王立坤, 钟志华. 自锁托槽矫治器应用于牙周病患者正畸治疗对牙周指数及龈沟液中IL-1β和TNF-α表达的影响[J]. 实用口腔医学杂志, 2018, 34(5): 656-659.
[2] Smith PC.Role of myofibroblasts in normal and pathological periodontal wound healing[J]. Oral Dis, 2018, 24(1-2): 26-29.
[3] 张莉, 李卫民. 不同龈下菌斑清除方式对龈沟液中细胞凋亡因子、炎症因子及MMPs/TIMPs平衡的影响[J]. 实用口腔医学杂志, 2019, 35(2): 289-292.
[4] 熊亚芳, 朱妮蔓, 杨芳. 不同正畸治疗措施对错畸形患者龈沟液MMP-2、TIMP-2水平的影响分析[J]. 口腔医学研究, 2018, 34(5): 544-547.
[5] Möhlhenrich SC, Brandt M, Kniha K, et al.Accuracy of orthodontic mini-implants placed at the anterior palate by tooth-borne or gingiva-borne guide support: a cadaveric study[J]. Clin Oral Investig, 2019, 23(12): 4425-4431.
[6] 陈潇, 刘旭倩, 张海燕. 牙龈成纤维细胞作为种子细胞在组织工程研究中的应用进展[J]. 医学综述, 2017, 58(13): 2531-2534.
[7] 高颖, 郝晨笛. 慢性牙周炎患者唾液中miR-146a的表达及其与龈沟炎症、MMP-8/TIMP-1水平的关系[J]. 上海口腔医学, 2018, 27(3): 309-312.
[8] Ghighi M, Llorens A, Baroukh B, et al.Differences between inflammatory and catabolic mediators of peri-implantitis and periodontitis lesions following initial mechanical therapy: an exploratory study[J]. J Periodontal Res, 2018, 53(1): 29-39.
[9] 邬扬绚, 褚金海, 刘秋红. 正畸治疗对牙龈蛋白R变化的影响及引发牙龈炎症的风险分析[J]. 河北医科大学学报, 2019, 40(10): 1185-1188.
[10] 黄晶, 杨文华. 脂联素对人牙龈成纤维细胞生物学作用的研究[J]. 中国急救医学, 2017, 37(2): 96-97.
[11] Xu X, Liu C, Zheng Y.3D tooth segmentation and labeling using deep convolutional neural networks[J]. IEEE Trans Vis Comput Graph, 2019, 25(7): 2336-2348.
[12] 赖欣添, 夏一如, 谢玉峰,等. 外源性ATP对牙龈成纤维细胞NLRP3炎症小体活化的影响[J]. 上海口腔医学, 2018, 27(4): 342-348.
[13] 樊宇, 莫水学. 机械压应力对人牙龈成纤维细胞表达钙网蛋白的影响[J]. 广西医科大学学报, 2018, 35(3): 294-297.

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