Changes of oral bacteria flora after wearing complete denture

doi:10.19439/j.sjos.2018.01.013

Shanghai Journal of Stomatology ›› 2018, Vol. 27 ›› Issue (1): 56-60.doi: 10.19439/j.sjos.2018.01.013

• Orginal Articles • Previous Articles Next Articles

Changes of oral bacteria flora after wearing complete denture

YU Wei-qiang¹, TIAN Fei², GUO Xiao-kui², ZHANG Fu-qiang¹, YE Dong-xia³

1.Department of Prosthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011
2.Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine. Shanghai 200025
3. Shanghai Research Institute of Stomatology. Shanghai, 200011, China

Received:2017-04-13 Revised:2017-08-15 Online:2018-02-25 Published:2018-03-05

Abstract

Abstract: PURPOSE: This study investigateed the influence of complete denture on oral bacteria flora. METHODS: Bateria plaque samples in oral mucosa, saliva and denture surfaces in 11 edentulous patients were collected, then the 16S rRNA gene clone libraries were constructed. Pyrosequencing was used to analyze the 16S rRNA gene V3~V4 regions, oral bacteria flora were classified and identified. RESULTS: There were 64800 sequences in complete denture-wearing subjects, Streptococcus mitis, Gemella haemolysans, Rothia mucilaginosa, Porphyromonas sp, Neisseria zoodegmatis, Granulicatella elegans, Acinetobacter baumannii, Pseudomonas citrinum, Granulicatella adiacens and Fusobacterium canifelinum were the predominant species (37416 sequences). The species of denture tissue surface were similar to these of buccal vestibule after wearing denture, and the species of denture smooth surface were similar to these of tongue ventrum and the floor of mouth. CONCLUSIONS: The effect of complete denture on oral flora is still limited, and the composition of oral flora is influenced by many other factors.

Key words: Complete denture, Oral bacteria flora, 16S rRNA

CLC Number:

R783

YU Wei-qiang, TIAN Fei, GUO Xiao-kui, ZHANG Fu-qiang, YE Dong-xia. Changes of oral bacteria flora after wearing complete denture[J]. Shanghai Journal of Stomatology, 2018, 27(1): 56-60.

References

[1] Xu J.Microbial ecology in the age of genomics and metagenomics: concepts, tools, and recent advances[J]. Mol Ecol, 2006, 15(7): 1713-1731.
[2] 张松梅, 田非, 黄庆丰, 等. 基于16基因序列分析1例健康人龈上菌群[J]. 中国微生态学杂志, 2011, 23(1): 44-48.
[3] Huang XL, Exterkate RA, ten Cate JM. Factors associated with alkali production from arginine in dental biofilms[J]. J Dent Res, 2012, 91(12): 1130-1134.
[4] Zhang SM, Tian F, Huang QF, et al.Bacterial diversity of subgingival plaque in 6 healthy Chinese individuals[J]. Exp Ther Med, 2011, 2(5): 1023-1029.
[5] Zhang SM, Tian F, Jiang X, et al.Evidence for calcifying nanoparticles in gingival crevicular fluid and dental calculus in periodontitis[J]. J Periodontol, 2009, 80(9): 1462-1470.
[6] Keijser BJ, Zaura E, Huse SM, et al.Pyrosequencing analysis of the oral microflora of healthy adults[J]. J Dent Res, 2008, 87(11): 1016-1020.
[7] Yu W, Qian C, Zhang S, et al.Effects of lipopolysaccharides on the corrosion behavior of Ni-Cr and Co-Cr alloys[J]. J Prosthet Dent, 2016, 116(2): 286-291.
[8] Zhang S, Qiu J, Ren Y, et al.Reciprocal interaction between dental alloy biocorrosion and Streptococcus mutans virulent gene expression[J]. J Mater Sci Mater Med, 2016, 27(4):78
[9] Aas JA, Paster BJ, Stokes LN, et al.Defining the normal bacterial flora of the oral cavity[J]. J Clin Microbiol, 2005, 43(11): 5721-5732.
[10] 张松梅, 田菲, 李静, 等. 对纳米细菌在牙结石形成过程中的作用的初步研究[J]. 中国微生态学杂志, 2009, 21(2): 152-155.
[11] Campos MS, Marchini L, Bernardes LA, et al.Biofilm microbial communities of denture stomatitis[J]. Oral Microbiol Immunol, 2008, 23(5): 419-424.
[12] Zhang SM, Qiu J, Tian F, et al.Corrosion behavior of pure titanium in the presence of Actinomyces naeslundii[J]. J Mater Sci Mater Med, 2013, 24(5): 1229-1237.
[13] Huang XL, Schulte RM, Burne RA, et al.Characterization of the arginolytic microflora provides insights into ph homeostasis in human oral biofilms[J]. Caries Res, 2015, 49(2): 165-176.
[14] Zhao X, Pan J, Zhang S, et al.Effectiveness of resin-based materials against erosive and abrasive enamel wear[J]. Clin Oral Investig, 2017, 21(1): 463-468.
[15] Zhou J, Huang Q, Wang X, et al.Early loading of splinted implants in the posterior mandible: a prospective multicentre case series[J]. J Clin Periodontol, 2016, 43(3): 298-304.
[16] 张松梅. 基于16S rRNA基因序列对口腔菌群组成的初步研究[D]. 上海: 上海交通大学: 2009.
[17] 熊薇, 张文池. 鲍曼不动杆菌的耐药性[J]. 中国抗感染化疗杂志, 2001, 1(2): 96-97.
[18] Sachdeo A, Haffajee AD, Socransky SS.Biofilms in the edentulous oral cavity[J]. J Prosthodont, 2008, 17(5): 348-356.
[19] Zhao YF, Zeng DL, Zhang SM, et al.Osteogenic potential of bone marrow stromal cells derived from streptozotocin-induced diabetic rats[J]. Int J Mol Med, 2013, 31(3): 614-620.
[20] Hope CK, Wilson M.Biofilm structure and cell vitality in a laboratory model of subgingival plaque[J]. J Microbiol Methods, 2006, 66(3): 390-398.
[21] Zhu X, Wang S, Gu Y, et al.Possible variation of the human oral bacterial community after wearing removable partial dentures by DGGE[J]. World J Microbiol Biotechnol, 2012, 28(5): 2229-2236.

Changes of oral bacteria flora after wearing complete denture

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Recommended Articles

Metrics

Comments

[1]	DENG Yu-wei, WEN Jin, ZHAO Jun, CHENG Hui-juan. Satisfaction and clinical assessment of 56 adult patients with biofunctional complete denture [J]. Shanghai Journal of Stomatology, 2021, 30(3): 328-331.
[2]	GUO Tian-wen, XING He-lin, WANG Jing, WANG Bao-cheng. Summary of 61 cases of removable denture restoration in children with congenital tooth deficiency [J]. Shanghai Journal of Stomatology, 2019, 28(2): 196-200.
[3]	HU Xiao-ping,ZHU Hong-shui,YU Jing-wen. The influence of lower vertical dimension construction of complete denture on cerebral blood flow [J]. Shanghai Journal of Stomatology, 2015, 24(1): 111-114.
[4]	ZHONG Qun, PENG Yan, WU Xue-ying, XIA Meng-pei. Photoelastic analysis of mandibular edentulous alveolar bone with denture adhesives [J]. Shanghai Journal of Stomatology, 2014, 23(1): 46-50.
[5]	JIAO Ji-lan, ZENG Li-wei, ZHOU Nian-gou, DENG Lu, CHEN Ping. Clinical evaluation of denture adhesive combined with oral wetting spray on satisfaction of complete denture in xerostomia patients [J]. Shanghai Journal of Stomatology, 2013, 22(5): 585-588.