Comparative study of submucosal microbial ecological environment between human peri-implantitis and healthy implants

doi:10.19439/j.sjos.2020.02.018

Abstract

Abstract: PURPOSE: To investigate the balance of submucosal microbial ecological environment in human peri-implantitis and healthy implants. METHODS: The patients with peri-implantitis and dental implants in Beijing Stomatological Hospital, and followed up for one year from September 2017 to January 2019 were enrolled. Patients were divided into two groups according to the presence or absence of peri-implantitis, i.e. peri-implantitis group (43, 51 implants) and healthy group (59 patients, 63 implants). The probing depth, bone resorption, probing bleeding, mPLI and mSBI were recorded. DNA of the submucosal microflora was extracted using a microbial gene extraction kit and sequenced by Illumina's MiSeq sequencing platform. Logistic regression analysis was used to analyze the correlation between the dominant bacteria and the average maximum probing depth, mPLI grading and MSBI grading in peri-implantitis group. The data were processed by SPSS 23.0 software package. RESULTS: The average maximum probing depth of peri-implantitis group was significantly higher than that in healthy group (P<0.05). The proportion of mPLI grade 2 in peri-implantitis group was significantly higher than that in healthy group [56.86%(29/51) vs 0.00%(0/63), P<0.05]. The proportion of mSBI grade 2 in peri-implantitis group was significantly higher than that in healthy group [60.78%(31/51) vs 0.00%(0/63), P<0.05]. The Simpson index of peri-implantitis group was significantly higher than that of the healthy group, and the Shannon index was significantly lower than the healthy group (P<0.05). In peri-implantitis group, the dominant microflora were Selenomonas (16.35%), Pseudomonas (11.20%), Fusobacterium (10.05%), Streptococcus (7.63%) and Treponema (6.84%). CONCLUSIONS: Compared with healthy implants, submucosal microorganisms of peri-implantitis have high species abundance and low species evenness, Pseudomonas, Selenomonas, Fusobacterium, Streptococcus and Treponema account for high proportions in the dominant bacteria.

Key words: Peri-implantitis, Probing depth, Microbes, Ecological balance

CLC Number:

R782.1

ZHAO Xiang, GE Hua-bing, FENG Jie, DU Yan. Comparative study of submucosal microbial ecological environment between human peri-implantitis and healthy implants[J]. Shanghai Journal of Stomatology, 2020, 29(2): 208-212.

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