Long non-coding RNA PCAT-1 expression in oral squamous cell carcinoma and its clinical significance

doi:10.19439/j.sjos.2019.01.013

Abstract

Abstract: PURPOSE: To investigate the correlation between the expression of lncRNA PCAT-1 and clinicopathological features of oral squamous cell carcinoma. METHODS: In this study, 112 oral tissue specimens, including 60 oral squamous cell carcinoma, and 52 non-cancer oral tissues were collected from Department of Stomatology in Anhui Provincial Hospital during 2013 to 2016. Reverse transcription quantitative PCR (RT-PCR) was performed to detect the expression of PCAT-1 and c-Myc. The correlation between the levels of PCAT-1 and clinical features (age, gender, high risk habit, histological stage, cervical lymphatic metastasis, differentiation) were analyzed. Graphpad Prism 7.0 software package was used for statistical analysis. RESULTS: Significant different levels of PCAT-1 were found between the high risk habit (alcohol and tobacco) group and low risk habit group, between patients with cervical lymphnode metastasis and patients without cervical lymphnode metastasis. The expression of PCAT-1 and c-Myc were up-regulated in OSCC, and there were positive correlation between the expression of PCAT-1 and c-Myc; up-regulated expression of PCAT-1 may be associated with higher morbidity of OSCC. CONCLUSIONS: PCAT-1 is overexpressed in oral squamous cell carcinoma and is associated with higher morbidity of OSCC.

Key words: Long non-coding RNA, PCAT-1, c-Myc, Oral squamous cell carcinoma

CLC Number:

R739.8

YANG Zong-cheng, HUANG Hai-yan, XU Xin. Long non-coding RNA PCAT-1 expression in oral squamous cell carcinoma and its clinical significance[J]. Shanghai Journal of Stomatology, 2019, 28(1): 67-70.

References

[1] Wu Z, Liu X, Liu L, et al.Regulation of lncRNA expression[J]. Cell Mol Biol Lett, 2014, 19(4): 561-575.
[2] Cui WC, Wu YF, Qu HM.Up-regulation of long non-coding RNA PCAT-1 correlates with tumor progression and poor prognosis in gastric cancer[J]. Eur Rev Med Pharmacol Sci, 2017, 21(13): 3021-3027.
[3] Qiao L, Liu X, Tang Y, et al.Down regulation of the long non-coding RNA PCAT-1 induced growth arrest and apoptosis of colorectal cancer cells[J]. Life Sci, 2017, 188: 37-44.
[4] Xu W, Chang J, Du X, et al.Long non-coding RNA PCAT-1 contributes to tumorigenesis by regulating FSCN1 via miR-145-5p in prostate cancer[J]. Biomed Pharmacother, 2017, 95: 1112-1118.
[5] Zhang D, Cao J, Zhong Q, et al.Long noncoding RNA PCAT-1 promotes invasion and metastasis via the miR-129-5p-HMGB1 signaling pathway in hepatocellular carcinoma[J]. Biomed Pharmacother, 2017, 95: 1187-1193.
[6] Ren Y, Shang J, Li J, et al.The long noncoding RNA PCAT-1 links the microRNA miR-215 to oncogene CRKL-mediated signaling in hepatocellular carcinoma[J]. J Biol Chem, 2017, 292(43): 17939-17949.
[7] Yang F, Xue X, Bi J, et al.Long noncoding RNA CCAT1, which could be activated by c-Myc, promotes the progression of gastric carcinoma[J]. J Cancer Res Clin Oncol, 2013, 139(3): 437-445.
[8] 单廷, 陈义钢, 洪波, 等. 长链非编码RNA PCAT-1在胃癌中的表达及临床意义[J]. 中华医学杂志, 2017, 97(18): 1411-1414.
[9] Deng L, Yang SB, Xu FF, et al.Long noncoding RNA PCAT-1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge[J]. J Exp Clin Cancer Res, 2015, 34: 18.
[10] He X, Tan X, Wang X, et al.C-Myc-activated long noncoding RNA PCAT-1 promotes colon cancer cell proliferation and invasion[J]. Tumour Boil, 2014, 35(12): 12181-12188.
[11] Zhang XF, Liu T, Li Y, et al.Overexpression of long non-coding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with breast cancer[J]. Int J Clin Exp Pathol, 2015, 8(8): 9440-9445.
[12] 韩勖, 凌志新, 胡强, 等. 长链非编码RNA PCAT-1对前列腺癌PC-3细胞增殖、迁移及凋亡的影响[J]. 东南大学学报(医学版), 2017, 36(1): 1-4.
[13] Lu L, Xu H, Luo F, et al.Epigenetic silencing of miR-218 by the lncRNA PCAT-1, acting via BMI1, promotes an altered cell cycle transition in the malignant transformation of HBE cells induced by cigarette smoke extract[J]. Toxicol Appl Pharmacol, 2016, 304: 30-41.
[14] Zhou B, Wang Y, Jiang J, et al.The long noncoding RNA colon cancer-associated transcript-1/miR-490 axis regulates gastric cancer cell migration by targeting hnRNPA1[J]. IUBMB Life, 2016, 68(3): 201-210.
[15] Chen L, Wang W, Cao L, et al.Long Non-Coding RNA PCAT-1 acts as a competing endogenous RNA to regulate cell growth and differentiation in acute myeloid leukemia[J]. Mol Cells, 2016, 39(4): 330-336.
[16] Chen C, Ridzon DA, Broomer AJ, et al.Realtime quantification of microRNAs by stem-loop RT-PCR[J]. Nucleic Acids Res, 2005, 33(20): e179.
[17] Kang K, Zhang X, Liu H, et al.A novel real-time PCR assay of microRNAs using S-Poly(T), a specific oligo(dT) reverse transcription primer with excellent sensitivity and specificity[J]. PLoS One, 2012, 7(11): e48536.
[18] Xiang JF, Yin QF, Chen T, et al.Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus[J]. Cell Res, 2014, 24(5): 513-531.
[19] Li J, Han L, Roebuck P, et al.An interactive open platform to explore the function of lncRNAs in cancer[J]. Cancer Res, 2015, 75(18): 3728-3737.
[20] Ge X, Chen Y, Liao X, et al.Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer[J]. Med Oncol, 2013, 30(2): 588.