Effect of serum starvation and culture to confluence on cell cycle synchronization and mineralization of human dental pulp cells

doi:10.19439/j.sjos.2019.01.001

Shanghai Journal of Stomatology ›› 2019, Vol. 28 ›› Issue (1): 1-5.doi: 10.19439/j.sjos.2019.01.001

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Effect of serum starvation and culture to confluence on cell cycle synchronization and mineralization of human dental pulp cells

PENG Wei-wei, DAI Zhao-wei, CAO Ying, HAN Jun-li, ZHU Ya-qin

Department of General Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2018-09-07 Revised:2018-11-10 Online:2019-02-25 Published:2019-04-12

Abstract

Abstract: PURPOSE: To compare the effect of serum starvation and culture to confluence on cell cycle synchronization and mineralization of human dental pulp cells (hDPCs). METHODS: HDPCs were cultured to 80% and 100% confluence respectively, and then cultured for 24, 48 and 72 hours by culture medium containing 0.5% fetal bovine serum(FBS). Cell cycle of hDPCs were identified by flow cytometry. Then hDPCs cultured by serum starvation for 48h after culturing to 100% confluence were used as the experimental group, and hDPCs cultured to 80% confluence were used as the control group. The expression of alkaline phosphatase(ALP), collagen type Ⅰ(COL-Ⅰ) and osteocalcin(OCN) was detected at gene level; activity of ALPase was detected at protein level. SPSS 13.0 software was used for statistical analysis. RESULTS: When hDPCs were cultured by serum starvation for 48h after culturing to 100% confluence, cells at G0/G1 stage were more than culture to 100% confluence and serum starvation group (P<0.05). At the genetic level, the expression of COL-Ⅰand OC in the experimental group was not statistically different from that of the control group, but can promote the expression of ALP(P<0.05), and stimulate the secretion of hDPCs at protein level at the same time (P<0.05). CONCLUSIONS: Culture to confluence combined serum starvation can synchronize more hDPCs at G0/G1 stage and promote mineralization of hDPCs.

Key words: Dental pup cell, Serum starvation, Culture to confluence, Cell cycle

CLC Number:

R781.3

PENG Wei-wei, DAI Zhao-wei, CAO Ying, HAN Jun-li, ZHU Ya-qin. Effect of serum starvation and culture to confluence on cell cycle synchronization and mineralization of human dental pulp cells[J]. Shanghai Journal of Stomatology, 2019, 28(1): 1-5.

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Effect of serum starvation and culture to confluence on cell cycle synchronization and mineralization of human dental pulp cells

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