Cytocompatibility of Co-Cr ceramic alloys after recasting

doi:10.19439/j.sjos.2017.03.002

Shanghai Journal of Stomatology ›› 2017, Vol. 26 ›› Issue (3): 246-250.doi: 10.19439/j.sjos.2017.03.002

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Cytocompatibility of Co-Cr ceramic alloys after recasting

LIN Hong-lei^{1, 2}, JIANG Lei^{1, 2}, ZHANG Chang-yuan^{1, 2, 3}, LIN Dong-hong⁴, CHENG Hui^{1, 2, 3}

1.School of Stomatology, Fujian Medical University. Fuzhou 350004;
2.Hospital of Stomatology, Fujian Medical University. Fuzhou 350002;
3.Research Centre of Dental Biomechanics & Aesthetic,
4. College of Medical Technology and Engineering, Fujian Medical University. Fuzhou 350004, Fujian Province, China ;

Received:2016-10-21 Revised:2016-12-05 Online:2017-06-25 Published:2017-07-05

Abstract

Abstract: PURPOSE: To evaluate the cytocompatibility of Co-Cr ceramic alloys after recasting. METHODS: Cobalt-chromium (Co-Cr) ceramic alloys were recasted for 1~3 times. Samples of Co-Cr ceramic alloys were immersed in DMEM culture medium. The concentration of ions of leaching liquor was determined by ICP-MS total quantization method. L-929 cells were cultured with leaching liquor, flow cytometry was used to test cell cycle progression, Annexin-V-FITC/PI apoptosis staining kit was used to quantitatively detect cell death patterns. SPSS 13.0 software package was used for statistical analysis. RESULTS: After casting for 1~3 times, no significant increase in the concentration of ions of Co-Cr ceramic alloys was noted (P>0.05) except Ga (P<0.05) . No significant differences in L-929 cell cycle were found in Co-Cr ceramic alloys between the samples cast for 1 time and for 2~3 times (P>0.05). No significant difference in the rates of apoptosis and necrosis was found (P>0.05). CONCLUSIONS: The biocompatibility of Co-Cr ceramic alloys casted for 1~3 times is not changed.

Key words: Co-Cr ceramic alloys, Recasting, Ion release, Cell cycle, Cell apoptosis

CLC Number:

R783.1

LIN Hong-lei, JIANG Lei, ZHANG Chang-yuan, LIN Dong-hong, CHENG Hui. Cytocompatibility of Co-Cr ceramic alloys after recasting[J]. Shanghai Journal of Stomatology, 2017, 26(3): 246-250.

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Cytocompatibility of Co-Cr ceramic alloys after recasting

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