外周血单个核细胞对口腔鳞癌细胞生长的影响

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (2): 146-149.

外周血单个核细胞对口腔鳞癌细胞生长的影响

刘琪^1*，孙祖俊²

1.上海市宝山区中西医结合医院检验科,上海 201900；
2.上海交通大学医学院附属第九人民医院检验科,上海 200011

收稿日期:2015-07-14 修回日期:2015-09-15 出版日期:2016-03-25 发布日期:2016-05-06
作者简介:刘琪(1979-),女,学士,主管技师,E-mail: sunyu0816@sina.com 孙祖俊,E-mail: sunzujun1976@126.com
基金资助:
国家自然科学基金(81001205)

The effect of human PBMC on the growth of oral squamous cell carcinoma cell

LIU Qi¹, SUN Zu-jun²

1.Department of Clinical Laboratory, Baoshan Traditional Chinese and Western Medicine Hospital. Shanghai 201900;
2.Department of Clinical Laboratory, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200011, China

Received:2015-07-14 Revised:2015-09-15 Online:2016-03-25 Published:2016-05-06

摘要/Abstract

摘要： 目的:研究活化人外周血单个核细胞(peripheral blood mononuclear cell,PBMC)在体外影响口腔鳞癌细胞生长及凋亡的机制。方法:用酶联免疫吸附试验(ELISA)、反转录聚合酶链反应(RT-PCR)、四甲基偶氮唑蓝(MTT)法以及流式细胞仪(FCM)检测植物血凝素(phytohemagglutinin, PHA)刺激前、后人PBMC分泌细胞因子变化及其对口腔鳞癌细胞生长与凋亡的影响。采用SPSS17.0软件包对数据进行统计学分析。结果:以PHA刺激人PBMC为实验组分泌的细胞因子显著高于对照组(P<0.01);实验组能够诱导颗粒酶B及穿孔素mRNA的表达;实验组抑制口腔肿瘤细胞生长及诱导口腔鳞癌细胞凋亡也显著增加(P<0.05)。结论:PHA激活的人PBMC在体外能够显著抑制口腔鳞癌细胞生长及诱导口腔鳞癌细胞凋亡。

关键词: 外周血单个核细胞, 植物血凝素, 口腔鳞癌

Abstract: PURPOSE: To explore the effect and mechanism of activated peripheral blood mononuclear cell (PBM) on the growth and apoptosis of oral squamous carcinoma cells in vitro. METHODS: ELISA, RT-PCR, MTT and flow cytometry were used to detect cytokines secretion and oral squamous carcinoma cell growth and apoptosis affected by activated PBMCs with PHA. The data was analyzed using SPSS17.0 software pakage. RESULTS: The cytokines secretion, mRNA expression of granzyme B and perforin, inhibition of growth and induction of apoptosis of oral squamous carcinoma cells treated by the activated PBMCs with PHA were significantly higher than the control groups (P<0.05). CONCLUSIONS: The human PBMC can be activated effectively with the stimulations of PHA, which can significantly inhibit growth and induce apoptosis of oral squamous carcinoma cells in vitro.

Key words: PBMC, PHA, Oral squamous cell carcinoma

中图分类号:

R739.8

刘琪，孙祖俊. 外周血单个核细胞对口腔鳞癌细胞生长的影响[J]. 上海口腔医学, 2016, 25(2): 146-149.

LIU Qi, SUN Zu-jun. The effect of human PBMC on the growth of oral squamous cell carcinoma cell[J]. Shanghai Journal of Stomatology, 2016, 25(2): 146-149.

参考文献

Kademani D. Oral cancer . Mayo Clin Proc, 2007, 82(7): 878-887.
Rini B. Future approaches in immunotherapy . Semin Oncol, 2014, 41(Suppl 5): S30-40.
Malek TR. The biology of interleukin-2 . Annu Rev Immunol, 2008, 26: 453-479. Boyman O, Purton JF, Surh CD, et al. Cytokines and T-cell homeostasis . Curr Opin Immunol, 2007, 19(3): 320-326.
闫轶鹏, 张青宜, 贺立山. 白血病患者化疗后应用IL-2对T淋巴细胞亚群的影响.细胞与分子免疫学杂志, 2010, 26(9): 898-899.
Krieg C, Létourneau S, Pantaleo G, et al. Improved IL-2 immunotherapy by selective stimulation of IL-2 receptors on lymphocytes and endothelial cells . Proc Natl Acad Sci USA, 2010, 107(26): 11906-11911.
Parmar S, Platanias LC. Interferons: mechanisms of action and clinical applications . Curr Opin Oncol, 2003, l5(6): 431-439.
Hu SX, Wang S, Wang JP, et al. Expression of endogenous granzyme B in a subset of human primary breast carcinomas . Br J Cancel, 2003, 89(1): 135-139.
石群立, 周晓军, 孟奎, 等. 穿孔素与颗粒酶B在鼻NK/T细胞淋巴瘤诊断中的意义 .临床与实验病理学杂志, 2004, 20(5): 580-584.
Lehhman C, Zeis M, Schmitz N, et al. Impaired binding of perforin the surface of tumor cells is a cause of target cell resistance against cytotoxic effector cells . Blood, 2000, 96(2):594-600.
Spitzer JH, Meadow GG. Modulation of perforin, granzyme A, and granzyme B in murine natural killer (NK), IL-2 stimulated NK, and lymphokine-activated killer cells by alcohol consumption . Cell Immunol, 1999, 194(2): 205-212.
Lehmann C, Zeis M, Uharek L. Activation of natural killer cells with interleukin 2 (IL-2) and IL-12 increases perforin binding and subsequent lysis of tumour cells . Br J Heamatol,2001, 114(3): 660-665.

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