上海口腔医学

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (2): 136-140.

• 论著 • 上一篇    下一篇

颞下颌关节盘前移位后VEGF-C基因在髁突软骨中的表达及意义

徐高丽1*，汪淑华1*，史秋涛1*，柳毅1*，阎帆1*，徐婷2，谷志远1   

  1. 1.浙江中医药大学 口腔医学院,浙江 杭州 310053；
    2.浙江大学医学院附属第一医院 口腔科,浙江 杭州 310003
  • 收稿日期:2015-07-16 修回日期:2015-09-23 出版日期:2016-03-25 发布日期:2016-05-06
  • 作者简介:徐高丽(1990-),女,在读硕士研究生,E-mail:852819264@qq.com;汪淑华(1989-),女,硕士,E-mail:385164787@qq.com。*并列第一作者 谷志远,E-mail: gzy@zju.edu.cn
  • 基金资助:
    国家自然科学基金(81170979,81400533);浙江省医药卫生科技计划项目(2015KYB146)

The expression of VEGF-C in the condylar cartilage after anterior disc displacement and its clinical significance

XU Gao-li1,WANG Shu-hua1,SHI Qiu-tao1,LIU Yi1,YAN Fan1,XU Ting2,GU Zhi-yuan1   

  1. 1.School of Stomatology, Zhejiang Chinese Medical University. Hangzhou 310053;
    2.Department of Stomatology, First hospital, Zhejiang University School of Medicine. Hangzhou 310003, Zhejiang Province, China
  • Received:2015-07-16 Revised:2015-09-23 Online:2016-03-25 Published:2016-05-06

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摘要： 目的:探讨血管内皮细胞生长因子C在颞下颌关节盘前移位后髁突软骨中的表达及意义。方法:采用日本大耳白兔60只,48只用弹力丝牵拉关节盘前移,建立关节盘前移模型,分别于术后1、2、4、8周处死12只,12只空白对照组同期处死,取出颞下颌关节标本。利用基因芯片技术进行筛选,实时荧光定量PCR进行验证和SP免疫组化技术检测不同组内髁突软骨中VGEF-C的表达与分布。采用SPSS19.0软件包对每组样本实验组和对照组数据进行配对t检验。结果:颞下颌关节盘前移位后,在基因水平上VEGF-C相比于正常对照组均有不同程度变化,早期VEGF-C表达减少,第2周时降至最低水平,差异显著(P<0.05);第8周时,VEGF-C表达有所增加。镜下观察可见VEGF-C在髁突软骨增殖层、肥大层及钙化软骨层中均有表达,1周组阳性表达主要见于增殖深层和肥大层,钙化软骨层也有弱阳性表达。2周组和4周组阳性表达主要位于肥大层,且在钙化软骨层中表达逐渐增强。8周组肥大层有阳性表达,钙化软骨层未见明显阳性表达,8周组与对照组相比有显著差异(P<0.05)。结论:关节盘前移位后早期髁突软骨VEGF-C基因的转录与表达有所下降,而在后期升高, 可能参与关节盘移位后髁突软骨的改建。

关键词: 关节盘前移, 髁突, VEGF-C, 基因芯片

Abstract: PURPOSE: To investigate the expression and significance of vascular endothelial growth factor-C in the condylar cartilage after anterior disc displacement of temporomandibular joint. METHODS: Sixty healthy adult Japanese rabbits were used in this study. Forty-eight rabbits were subjected to surgical anterior disc displacement in the right TMJs, every 12 rabbits were sacrificed 1, 2, 4, 8 weeks post-operation, 12 rabbits without operation were used as normal control.The gene expression and distribution of VEGF-C in condylar cartilage were studied by gene chip, real-time quantitative PCR and SP immunohistochemical methods. Paired t test was used for analysis with SPSS19.0 software package. RESULTS: After anterior disc displacement, the expression of VEGF-C in the 2nd week reached to a minimum level, there was a significant difference (P<0.05), and increased in the 8th week. VEGF-C was expressed by cells of articular cartilage in the proliferative zone, hypertrophic zone and calcified cartilage zone. The expression in 1-week group was mainly found in the deep proliferative zone and hypertrophic zone, while the expression mainly found in the hypertrophic zone in 2-and 4-week group; calcified cartilage gradually enhanced, and there was significant difference (P<0.05) between 8-week group and control group. CONCLUSIONS: VEGF-C gene transcription and expression may be involved in the remodeling of the condylar cartilage after anterior disc displacement.

Key words: Anterior disc displacement, Mandibular condyle, VEGF-C , Gene chip

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