ADAM28反义核酸对人牙龈成纤维细胞生物学功能的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (5): 524-530.

ADAM28反义核酸对人牙龈成纤维细胞生物学功能的影响

赵征, 魏立, 李露嘉, 叶勇, 黄征难, 杨海青

解放军第401医院口腔科,山东青岛 266071

收稿日期:2014-05-12 修回日期:2014-07-09 出版日期:2014-10-20 发布日期:2015-02-04
通讯作者: 赵征,Tel:0532-51870540,E-mail:zhaozheng170@163.com
作者简介:赵征（1973-）,女,口腔医学博士、博士后,副主任医师,硕士研究生导师
基金资助:
中国博士后科学基金特别资助课题（201003774）

The effects of ADAM28 AS-ODN on biological function of human gingival fibroblasts

ZHAO Zheng, WEI Li, Li Lu-jia, YE Yong, HUANG Zheng-nan, YANG Hai-qing

Department of Stomatology, the 401 Hospital of Chinese PLA. Qingdao 266071, Shandong Province, China)

Received:2014-05-12 Revised:2014-07-09 Online:2014-10-20 Published:2015-02-04
Supported by:
; Supported by Chinese Post-Doctoral Special Science Foundation (201003774)

摘要/Abstract

摘要： 目的研究金属蛋白酶解离素28（a disintegrin and metalloproteinase 28, ADAM28）反义核酸（antisense oligodeoxynucleotide, AS-ODN）对人牙龈成纤维细胞（human gingival fibroblasts, HGFs）增殖、分化、凋亡的影响,并分析可能的作用机制。方法采用细胞培养、基因转染、四甲基唑蓝（MTT）比色法、酶动力学法和流式细胞术（FCM）检测ADAM28反义核酸转染HGFs后对细胞生物学特性的影响。采用SPSS16.0软件包中的SNK检验对数据进行统计学分析。结果 ADAM28 AS-ODN组HGFs的增殖活性显著降低。AS-ODN组HGFs处于S期的细胞百分比显著低于S-ODN组和未转染组,G₂+M期的细胞百分比显著低于未转染组,AS-ODN组的细胞增殖指数（PI=S+G₂M）显著低于其他2组,差异显著。AS-ODN组碱性磷酸酶（ALP）分泌活性、凋亡细胞百分比显著升高。结论 ADAM28 反义核酸可显著抑制HGFs的增殖,并影响细胞周期的变化,促进其分化,显著诱导HGFs的凋亡。

关键词: 金属蛋白酶解离素28, 反义核酸, 人牙龈成纤维细胞

Abstract: PURPOSE: To study the effects of a disintegrin and metalloproteinase 28 (ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human gingival fibroblasts (HGFs), and analyze the possible mechanism. METHODS: Cell culture, gene transfection, MTT chromatometry, enzyme dynamics, and flow cytometry (FCM) techniques were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HGFs after transfected into HGFs. The statistical differences were evaluated by SNK test with SPSS 16.0 software package. RESULTS: In ADAM28 AS-ODN group, the proliferation activity of HGFs decreased significantly. Cell percentage in S phase in AS-ODN group was notably lower than that of S-ODN and untransfected groups, and cell percentage in G₂+M phase was remarkably lower than that of untransfected group. Cell proliferation index（PI=S+G₂M）in AS-ODN group was significantly lower than that of the other two groups. There was a significant difference between the groups. In AS-ODN group, alkaline phosphatase (ALP) secretion activity and percentage of apoptotic cell notably increased. CONCLUSIONS: ADAM28 AS-ODN could inhibit HGFs proliferation significantly and influence the changes of cell cycle, promote HGFs differentiation and induce HGFs apoptosis remarkably.

Key words: ADAM28, AS-ODN, HGFs

赵征, 魏立, 李露嘉, 叶勇, 黄征难, 杨海青. ADAM28反义核酸对人牙龈成纤维细胞生物学功能的影响[J]. 上海口腔医学, 2014, 23(5): 524-530.

ZHAO Zheng, WEI Li, Li Lu-jia, YE Yong, HUANG Zheng-nan, YANG Hai-qing. The effects of ADAM28 AS-ODN on biological function of human gingival fibroblasts[J]. Shanghai Journal of Stomatology, 2014, 23(5): 524-530.

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