TiO2-PEEK-CRGDS材料制备及抗牙龈卟啉单胞菌活性和细胞活性分析

doi:10.19439/j.sjos.2022.01.011

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (1): 54-57.doi: 10.19439/j.sjos.2022.01.011

TiO₂-PEEK-CRGDS材料制备及抗牙龈卟啉单胞菌活性和细胞活性分析

许辛夷¹, 张银莲²

1.常州市中医医院口腔科,江苏常州 213003;
2.常州市口腔医院预防保健科,江苏常州 213003

收稿日期:2021-07-22 修回日期:2021-10-18 出版日期:2022-02-25 发布日期:2022-03-10
通讯作者: 张银莲,E-mail:38163518@qq.com
作者简介:许辛夷（1979-）,男,硕士,副主任医师,E-mail:xxy7956@sina.com

Analysis of material preparation, anti-bacterial activity of Porphyromonas gingivalis and cell bioassays of TiO₂-PEEK-CRGDS

XU Xin-yi¹, ZHANG Ying-lian²

1. Department of Stomatology, Changzhou Hospital of Traditional Chinese Medicine. Changzhou 213003;
2. Department of Prevention and Healthcare, Changzhou Hospital of Stomatology. Changzhou 213003, Jiangsu Province, China

Received:2021-07-22 Revised:2021-10-18 Online:2022-02-25 Published:2022-03-10

摘要/Abstract

摘要： 目的: 将聚醚醚酮(polyetheretherketone,PEEK)复合材料用纳米二氧化钛（TiO₂）和CRGDS肽改性,制备得到TiO₂-PEEK-CRGDS,评价其抗牙龈卟啉单胞菌活性和细胞活性。方法: 使用等离子体浸没离子注入法（plasma-immersion ion implantation,PⅢ）制备TiO₂-PEEK,再浸于CRGDS五肽的明胶溶液,冻干得到TiO₂-PEEK-CRGDS。以相同直径和厚度的PEEK作为对照,使用扫描电镜（SEM）观察各试件表面的三维形貌和粗糙度,通过菌落计数计算牙龈卟啉单胞菌的存活率,使用活/死细胞检测试剂盒检测人牙龈成纤维细胞(human gingival fibroblasts, HGFs)活性,利用CCK-8 法检测 HGFs的增殖能力。采用GraphPad Prism 7.0 软件作图,组间差异采用t检验。结果: PEEK、TiO₂-PEEK和TiO₂-PEEK-CRGDS的抑菌率分别为40.3%、57.2%和60.3%;12天时,TiO₂-PEEK-CRGDS促进人牙龈成纤维细胞增长能力是TiO₂-PEEK组的2.47倍,差异有统计学意义（P<0.05）。结论: 本研究优化得到的TiO₂-PEEK-CRGDS相对于纯钛、PEEK具有较好的亲水性,可显著促进牙龈成纤维细胞增殖。

关键词: TiO₂-PEEK-CRGDS, 牙龈卟啉单胞菌, 人牙龈成纤维细胞

Abstract: PURPOSE: To modify polyetheretherketone(PEEK) composite material with nanometer titanium dioxide (TiO₂) and CRGDS peptide to prepare TiO₂-PEEK-CRGDS, and evaluate its anti- activity of Porphyromonas gingivalis and effect on cell activity. METHODS: TiO₂-PEEK was prepared by plasma immersion ion implantation(PⅢ), then immersed into gelatin solution of pentapeptides of CRGDS, and freeze-dried. The same diameter and thickness of PEEK was set as control. Scanning electron microscope (SEM) was used to observe the three-dimensional topography and roughness on the surface of the specimen, colony count was calculated to evaluate the survival of Porphyromonas gingivalis, living/dead cell count was used to detect the activity of human gingival fibroblasts(HGFs), CCK-8 assay was used to detect the proliferation ability of HGFs. The quantitative data was indicated as and analyzed by using GraphPad Prism 7.0 software package. RESULTS: The antibacterial rates of PEEK, TiO₂-PEEK and TiO₂-PEEK-CRGDS were 40.3%, 57.2% and 60.3%, respectively. At 12 days, the ability of TiO₂-PEEK-CRGDS to promote the growth of human gingival fibroblasts was significantly different from that of the TiO₂-PEEK and PEEK group(P<0.05). CONCLUSIONS: The optimized TiO₂-PEEK-CRGDS has better hydrophilicity than pure titanium and PEEK, and can significantly promote the proliferation of gingival fibroblasts.

Key words: TiO₂-PEEK-CRGDS, Porphyromonas gingivalis, Human gingival fibroblasts

中图分类号:

R783.1

许辛夷, 张银莲. TiO₂-PEEK-CRGDS材料制备及抗牙龈卟啉单胞菌活性和细胞活性分析[J]. 上海口腔医学, 2022, 31(1): 54-57.

XU Xin-yi, ZHANG Ying-lian. Analysis of material preparation, anti-bacterial activity of Porphyromonas gingivalis and cell bioassays of TiO₂-PEEK-CRGDS[J]. Shanghai Journal of Stomatology, 2022, 31(1): 54-57.

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TiO₂-PEEK-CRGDS材料制备及抗牙龈卟啉单胞菌活性和细胞活性分析

Analysis of material preparation, anti-bacterial activity of Porphyromonas gingivalis and cell bioassays of TiO₂-PEEK-CRGDS

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