雷奈酸锶对大鼠快速扩弓过程中腭中缝BMP-2表达的影响

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (6): 647-651.

雷奈酸锶对大鼠快速扩弓过程中腭中缝BMP-2表达的影响

徐超^{1, 3}, 王旭霞^{2, 3}, 王英姿^{1, 3}, 杨盼盼^{1, 3}, 赵姝亚^{1, 3}, 张君^{1, 3}

1.山东大学口腔医学院正畸科,2.口腔颌面外科,山东济南 250012;
3.山东省口腔生物医学重点实验室,山东济南 250012

收稿日期:2015-12-25 出版日期:2016-12-25 发布日期:2016-12-29
通讯作者: 张君,Tel： 0531-88382069, E-mail: zhangj@sdu.edu.cn
作者简介:徐超（1990-）,男,在读硕士研究生,E-mail:1193126470@qq.com
基金资助:
国家自然科学基金（81371180）; 山东省科技发展计划（2014GGH218024）

Effects of strontium ranelate on the expression of BMP-2 in the mid-palatal suture of rats during rapid maxillary expansion

XU Chao^{1, 3}, WANG Xu-xia^{2, 3}, WANG Ying-zi^{1, 3}, YANG Pan-pan^{1, 3}, ZHAO Shu-ya^{1, 3}, ZHANG Jun^{1, 3}

1. Department of Orthodontics, 2.Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University. Jinan 250012;
3. Shandong Provincial Key Laboratory of Oral Biomedicine. Jinan 250012, Shandong Province, China

Received:2015-12-25 Online:2016-12-25 Published:2016-12-29

摘要/Abstract

摘要： 目的研究雷奈酸锶对大鼠快速扩弓过程中腭中缝骨形态发生蛋白2（bone morphogenetic protein-2,BMP-2）表达的影响。方法选取36只6周龄雄性Wistar大鼠,随机分为A、B、C 3组,每组12只,其中,A组为对照组。将机械扩弓装置置于B、C 2组大鼠上颌两侧第一、二磨牙之间,并加力100 g,A组不做处理。C组大鼠每天灌服600 mg/kg雷奈酸锶溶液,B组灌服相同剂量的生理盐水。分别于加力后4、7、10 d分批处死大鼠,用免疫组织化学染色法检测大鼠腭中缝组织中BMP-2的表达,并利用Image-pro plus 粘结对切片吸光度进行分析,采用SPSS19.0软件包对数据进行统计学分析。结果免疫组织化学染色显示,B组大鼠腭中缝组织中BMP-2的表达量在第4天时显著高于A组（P<0.05）,而在第7天和第10天时与A组无显著差异（P>0.05）;C组大鼠腭中缝组织中BMP-2的表达量在各个时间点均显著高于其余2组（P<0.05）。结论雷奈酸锶能够促进大鼠快速扩弓过程中BMP-2的表达量和表达时长,可能促进了快速扩弓过程中腭中缝的新骨形成。

关键词: 雷奈酸锶, BMP-2, 上颌快速扩弓

Abstract: PURPOSE: To investigate the effects of strontium ranelate on the expression of BMP-2 during rapid maxillary expansion. METHODS: Thirty-six male 6-week- old male Wistar rats were selected. They were randomly divided into 3 groups. Group A was designed as a control group. An expanded application producing 100 g force was fixed between the first and the second molar on both sides of the rats in group B and group C. 600 mg/kg strontium ranelate was given to the rats in group C daily via an orogastric route, while the equal normal saline was given to the rats in group B. Then the rats were sacrificed on day 4, 7 and 10. The expression of BMP-2 which is a sign of bone formation was detected with immunohistochemical staining and analyzed with Image-pro plus 5.0. SPSS19.0 software package was used for statistical analysis. RESULTS: The expression of BMP-2 in the midpalatal suture was significantly greater in group B than that in group A on day 4 (P<0.05); however, there was no significant difference between group A and group B on day 7 and 10 (P>0.05). The expression of BMP-2 in the midpalatal suture in group C was significantly greater than that in the other two groups at each time point (P<0.05). CONCLUSIONS: Strontium ranelate promotes the expression time and quantity of BMP-2 in the mid-palatal suture of rats during rapid maxillary expansion and may accelerate bone formation during rapid maxillary expansion.

Key words: Strontium ranelate, BMP-2, Rapid maxillary expansion

中图分类号:

R783.5

徐超, 王旭霞, 王英姿, 杨盼盼, 赵姝亚, 张君. 雷奈酸锶对大鼠快速扩弓过程中腭中缝BMP-2表达的影响[J]. 上海口腔医学, 2016, 25(6): 647-651.

XU Chao, WANG Xu-xia, WANG Ying-zi, YANG Pan-pan, ZHAO Shu-ya, ZHANG Jun. Effects of strontium ranelate on the expression of BMP-2 in the mid-palatal suture of rats during rapid maxillary expansion[J]. Shanghai Journal of Stomatology, 2016, 25(6): 647-651.

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