Wnt5a在慢性根尖周炎病损中的表达及临床意义

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (4): 470-474.

Wnt5a在慢性根尖周炎病损中的表达及临床意义

徐莉雅, 马楠, 仇丽鸿, 于雅琼, 钟鸣, 于静涛, 薛明

中国医科大学口腔医学院牙体牙髓病科,辽宁省口腔医学研究所牙体牙髓病研究室,辽宁省口腔疾病转化医学研究中心,辽宁沈阳 110002

收稿日期:2015-01-30 出版日期:2015-08-20 发布日期:2015-09-10
作者简介:徐莉雅(1983-),女,硕士研究生,住院医师,

Expression of Wnt5a in chronic apical periodontitis and its clinical significance

XU Li-ya, MA Nan, QIU Li-hong, YU Ya-qiong, ZHONG Ming, YU Jing-tao, XUE Ming

Department of Endodontics, School of Stomatology, China Medical University; Lab of Endodontics, Liaoning Provincial Institute of Stomatology; Liaoning Provincial Research Center of Translational Oral Medicine. Shenyang 110002, Liaoning Province, China

Received:2015-01-30 Online:2015-08-20 Published:2015-09-10

摘要/Abstract

摘要： 目的检测慢性根尖周炎根尖病损区Wnt5a的水平与细胞定位,探讨Wnt5a与炎症细胞浸润程度的关系,以及Wnt5a在慢性根尖周炎发展中的作用及意义。方法选取10例慢性根尖周炎牙作为实验组,根据根尖区组织的H-E染色,依炎症细胞浸润程度将病例组分为轻中度组、重度组。5例因正畸需要而拔除的健康牙作为对照组。采用实时定量PCR(real-time PCR,RT-PCR)从mRNA水平检测样本Wnt5a的表达;免疫组织化学法检测样本中Wnt5a蛋白的表达,应用图像分析软件检测Wnt5a蛋白表达水平,分析Wnt5a表达高低与炎症程度的关系。采用SPSS13.0软件包对数据进行统计学分析。结果Wnt5a在实验组与对照组均有表达,实验组中Wnt5a mRNA表达水平显著高于对照组(P<0.05)。免疫组织化学结果显示,重度炎症组Wnt5a的表达水平显著高于对照组(P<0.01);轻中度组Wnt5a的表达水平显著高于对照组(P<0.05);重度炎症组中Wnt5a的表达水平显著高于轻中度炎症组(P<0.05)。结论Wnt5a与慢性根尖周炎的炎症程度相关,可能参与了病变的始动过程,推测Wnt5a在慢性根尖周炎的发生、发展中起着一定作用。

关键词: 慢性根尖周炎, Wnt5a, 免疫组织化学, RT-PCR法

Abstract: PURPOSE: To detect the expression of Wnt5a in lesions of chronic apical periodontitis and determine the relationship between expression of Wnt5a and inflammation degree. METHODS: Ten patients with chronic apical periodontitis and 5 healthy controls were enrolled in this study. According to the inflammatory cell infiltration, the specimens were divided into 2 groups: severe inflammation group and mild inflammation group. The expression of Wnt5a was measured by real-time PCR (RT-PCR) and immunohistochemical analysis in the lesions of chronic apical periodontitis. The amount of Wnt5 expression was assayed and compared in different inflammation levels. Statistical analysis was performed using SPSS 13.0 software package. RESULTS: Wnt5a was detected in both groups. Expression of Wnt5a mRNA in patients were significantly higher than the controls (P<0.05). According to inflammation level, the positive expression rate of Wnt5a in severe inflammation group was significantly higher than the controls (P<0.01), and Wnt5a positive expression in mild inflammation group was also significantly higher than the controls (P<0.05). The expression of Wnt5a was significantly different between severe inflammation group and mild inflammation group (P<0.05). CONCLUSIONS: The expression of Wnt5a increases as the severity of tissue inflammation increases , which indicates that Wnt5a plays an important role in the development of chronic apical periodontitis.

Key words: Chronic apical periodontitis, Immunohistochemistry, Wnt5a, Real time-PCR Shanghai J Stomatol, 2015, 24(4):470-474.

中图分类号:

R783.5

徐莉雅, 马楠, 仇丽鸿, 于雅琼, 钟鸣, 于静涛, 薛明. Wnt5a在慢性根尖周炎病损中的表达及临床意义[J]. 上海口腔医学, 2015, 24(4): 470-474.

XU Li-ya, MA Nan, QIU Li-hong, YU Ya-qiong, ZHONG Ming, YU Jing-tao, XUE Ming. Expression of Wnt5a in chronic apical periodontitis and its clinical significance[J]. Shanghai Journal of Stomatology, 2015, 24(4): 470-474.

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