氟对大鼠切牙成釉细胞内质网伴侣分子表达的影响

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (5): 481-486.

• 基础研究 • 下一篇

氟对大鼠切牙成釉细胞内质网伴侣分子表达的影响

张凯强，张颖，刘璐，顾何锋，马林

(中国医科大学口腔医学院口腔预防科，辽宁省口腔医学研究所口腔预防教研室，辽宁沈阳 110002)

收稿日期:2013-02-14 修回日期:2013-04-10 出版日期:2013-10-10 发布日期:2013-10-10
通讯作者: 张颖，Tel：024-22895293，E-mail：zhangyingcmu@126.com
作者简介:张凯强(1987-)，男，在读硕士研究生，E-mail：zhangkaiqiang1208@gmail.com
基金资助:
国家自然科学基金(81072245)；辽宁省自然科学基金(20102278)

Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor

ZHANG Kai-qiang, ZHANG Ying, LIU Lu, GU He-feng, MA Lin

Department of Preventive Dentistry, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China

Received:2013-02-14 Revised:2013-04-10 Online:2013-10-10 Published:2013-10-10
Supported by:
Supported by National Natural Science Foundation of China (81072245) and Natural Science Foundation of Liaoning Province (20102278).

摘要/Abstract

摘要： 目的：研究不同浓度氟化物对大鼠切牙成釉细胞的影响，探讨内质网应激在氟斑牙形成中的作用。方法：30只Wistar大鼠随机分为3组，建立氟斑牙动物模型。观察大鼠切牙成釉细胞的形态学和GRP78、XBP-1、CRT和caspase-12表达的改变，采用MetaMorph显微图像分析软件和SPSS 13.0软件包对数据进行统计学分析。结果：随着饮水氟离子浓度的升高，切牙逐渐出现氟斑牙症状。免疫组化结果显示，CRT(F=11.72，P<0.05)、GRP78(F=27.42，P<0.05)、XBP-1(F=139.7，P<0.05)、caspase-12(F=43.91，P<0.05)表达随氟离子浓度的升高而升高,且各组间均具有显著性差异。结论：成釉细胞在一定浓度的氟化物作用下，其CRT、GRP78、XBP-1和caspase-12 均过表达，表明成釉细胞处于内质网应激状态，且caspase-12是导致细胞凋亡的重要途径。

关键词: 氟, 成釉细胞, 内质网应激

Abstract: PURPOSE: To investigate the effect of different concentrations of fluoride on the expression of endoplasmic reticulum chaperone, and to explore the mechanism of dental fluorosis in rat. METHODS: Thirty Wistar rats were randomly divided into 3 groups. Immunohistochemistry was used to detect the expression of CRT, GRP78, XBP-1 and caspase-12 in rat incisors. Metamorph microscope images analysis system and SPSS 13.0 software package was used to analyze the data. RESULTS: Typical features of dental fluorosis were found in the fluoride group. Results of immunohistochemistry showed that CRT (F=238.6, P<0.05), GRP78 (F=27.42, P<0.05), XBP-1 (F=139.7, P<0.05) and caspase-12 (F=43.91, P<0.05) were significantly different among the 3 groups. CONCLUSIONS: Excessive fluoride can increase the secretion of CRT, GRP78, XBP-1 and caspase-12 suggest the ameloblasts and in status of endoplasmic reticulum stress and caspase-12 plays an important role during ameloblast apoptosis.

Key words: Fluoride, Ameloblast, Endoplasmic reticulum stress

中图分类号:

R780.1

张凯强, 张颖, 刘璐, 顾何锋, 马林. 氟对大鼠切牙成釉细胞内质网伴侣分子表达的影响[J]. 上海口腔医学, 2013, 22(5): 481-486.

ZHANG Kai-qiang, ZHANG Ying, LIU Lu, GU He-feng, MA Lin. Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor[J]. Shanghai Journal of Stomatology, 2013, 22(5): 481-486.

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氟对大鼠切牙成釉细胞内质网伴侣分子表达的影响

Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor

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