EPA对P.gingivalis LPS诱导人牙周膜成纤维细胞炎症反应的影响

doi:10.19439/j.sjos.2023.05.005

上海口腔医学 ›› 2023, Vol. 32 ›› Issue (5): 475-479.doi: 10.19439/j.sjos.2023.05.005

EPA对P.gingivalis LPS诱导人牙周膜成纤维细胞炎症反应的影响

周子超¹, 束蓉^2,*, 吴轶群^1,*

1.上海交通大学医学院附属第九人民医院口腔第二门诊部,2.牙周病科,上海交通大学口腔医学院,国家口腔医学研究中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2021-11-25 修回日期:2022-06-24 出版日期:2023-10-25 发布日期:2023-11-03
通讯作者: 束蓉,E-mail：shurong123@hotmail.com;吴轶群,E-mail：yiqunwu@hotmail.com。^*共同通信作者
作者简介:周子超（1990-）,男,硕士,住院医师,E-mail：13917972906@163.com

Effect of EPA on inflammatory response of human periodontal ligament fibroblasts induced by P. gingivalis LPS

ZHOU Zi-chao¹, SHU Rong², WU Yi-qun¹

1. Department of 2nd Dental Center, 2. Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2021-11-25 Revised:2022-06-24 Online:2023-10-25 Published:2023-11-03

摘要/Abstract

摘要： 目的：分析二十碳五烯酸（eicosapntemacnioc acid, EPA）对牙龈卟啉单胞菌（Porphyromonas gingivalis, P.gingivalis）脂多糖（lipopolysaccharides, LPS）诱导人牙周膜成纤维细胞（human periodontal ligament cells, hPDLCs）分泌炎症因子的作用。方法：利用组织块法培养hPDLCs,MTT法检测不同浓度EPA对hPDLCs细胞活性的影响。根据MTT结果选择合适的EPA浓度,分别采用实时定量 PCR和ELISA法检测EPA对P.gingivalis LPS诱导hPDLCs分泌白细胞介素6（Interleukin-6, IL-6）、IL-8和IL-1β能力的影响,采用SPSS 10.0软件包对数据进行统计学分析。结果：25~100 μmol/L EPA对hPDLCs细胞活性无影响,但可呈剂量依赖性下调P.gingivalis LPS诱导hPDLCs分泌IL-6、IL-8和IL-1β 的能力。结论：EPA有望在不影响细胞活性的情况下,抑制P.gingivalis LPS诱导hPDLCs分泌炎症因子的能力,EPA可能成为牙周炎抗炎治疗的潜在靶点。

关键词: 二十碳五烯酸, 脂多糖, 人牙周膜成纤维细胞, 炎症因子

Abstract: PURPOSE: To observe the effect of eicosapentaenoc acid (EPA) on the expression of inflammatory factors in human periodontal ligament fibroblasts (hPDLCs) induced by using lipopolysaccharides (LPS) of Porphyromonas gingivalis (P. gingivalis). METHODS: hPDLCs were cultured by using tissue block method, and the effects of different concentrations of EPA on the activity of hPDLCs cells were observed by MTT method. According to MTT results, the appropriate concentration of EPA was selected, and the expression of interleukin-6(IL-6), IL-8 and IL-1β in hPDLCs induced by P. gingivalis LPS was detected by real time PCR and ELISA. The data were evaluated by SPSS 10.0 software package. RESULTS: 25-100 μmol/L EPA had no effect on the activity of hPDLCs cells, but could inhibit the expression of IL-6, IL-8 and IL-1β induced by P. gingivalis LPS in a dose-dependent manner. CONCLUSIONS: EPA can inhibit the expression of inflammatory factors induced by P. gingivalis LPS without affecting cell activity, indicating that EPA has the possibility of anti-inflammatory treatment of periodontitis.

Key words: Eicosapentaenoic acid, Lipopolysaccharide, Human periodontal ligament fibroblasts, Inflammatory factors

中图分类号:

R781.4

周子超, 束蓉, 吴轶群. EPA对P.gingivalis LPS诱导人牙周膜成纤维细胞炎症反应的影响[J]. 上海口腔医学, 2023, 32(5): 475-479.

ZHOU Zi-chao, SHU Rong, WU Yi-qun. Effect of EPA on inflammatory response of human periodontal ligament fibroblasts induced by P. gingivalis LPS[J]. Shanghai Journal of Stomatology, 2023, 32(5): 475-479.

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EPA对P.gingivalis LPS诱导人牙周膜成纤维细胞炎症反应的影响

Effect of EPA on inflammatory response of human periodontal ligament fibroblasts induced by P. gingivalis LPS

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