柯里拉京体内外诱导口腔鳞癌CAL-27细胞凋亡及机制探讨

doi:10.19439/j.sjos.2023.05.003

上海口腔医学 ›› 2023, Vol. 32 ›› Issue (5): 462-467.doi: 10.19439/j.sjos.2023.05.003

柯里拉京体内外诱导口腔鳞癌CAL-27细胞凋亡及机制探讨

李胜男^1,2, 崔飞艳², 赵姗², 杜琛², 孟箭^1,2

1.潍坊医学院口腔医学院,山东潍坊 261053;
2.徐州市中心医院口腔科,江苏徐州 221000

收稿日期:2022-05-20 修回日期:2022-07-17 出版日期:2023-10-25 发布日期:2023-11-03
通讯作者: 孟箭,E-mail: mrocket@126.com
作者简介:李胜男（1995-）,女,在读硕士研究生,医师,E-mail: 18863661105@163.com
基金资助:
国家口腔疾病临床医学研究中心开放性课题（NCRCO-202101）; 徐州市重点研发计划（KC21187）

Corilagin-induced apoptosis of oral squamous carcinoma CAL-27 cells in vitro and in vivo and its mechanism

LI Sheng-nan^1,2, CUI Fei-yan², ZHAO Shan², DU Chen², MENG Jian^1,2

1. School of Stomatology, Weifang Medical University. Weifang 261053, Shandong Province;
2. Department of Stomatology, Xuzhou Central Hospital. Xuzhou 221000, Jiangsu Province, China

Received:2022-05-20 Revised:2022-07-17 Online:2023-10-25 Published:2023-11-03

摘要/Abstract

摘要： 目的：观察柯里拉京（corilagin）对人舌鳞癌CAL-27细胞增殖和凋亡的作用,探讨诱导其凋亡的分子机制。方法：体外实验采用不同浓度的柯里拉京预处理CAL-27细胞,通过CCK-8法及平板克隆形成实验检测细胞增殖能力;流式细胞术检测细胞凋亡;qRT-PCR和Western 印迹法检测对细胞中Bax、Bcl-2、Caspase-3、Cleaved Caspase-3 mRNA及蛋白表达水平的影响。通过CAL-27细胞构建裸鼠移植瘤模型,检测柯里拉京的体内抗肿瘤效果。采用GraphPad Prism 8.0软件包对数据进行统计学分析。结果：体外实验结果显示,柯里拉京以浓度依赖性方式抑制CAL-27细胞增殖,诱导细胞凋亡;在mRNA和蛋白水平上上调Bax、Caspase-3和Cleaved Caspase-3,下调Bcl-2,差异均有统计学意义（P<0.05）。体内实验表明,与对照组相比,柯里拉京组可显著减小裸鼠瘤体体积（P<0.05）。结论：柯里拉京在体内和体外均能显著抑制人舌鳞癌CAL-27细胞增殖并促进其凋亡,其作用机制可能与调控Bax/Bcl-2/Caspase-3信号通路有关。

关键词: 柯里拉京, CAL-27细胞, 增殖, 凋亡, Bax/Bcl-2/Caspase-3信号通路

Abstract: PURPOSE: To investigate the effect of corilagin on proliferation and apoptosis of human oral squamous cell carcinoma CAL-27 cells, and to explore the molecular mechanism of inducing cell apoptosis. METHODS: In vitro experiments, Cal-27 cells were treated with different concentrations of corilagin, cell-counting kit-8(CCK-8) assay and colony formation assay were performed to evaluate cell proliferation; flow cytometric analysis was used to evaluate cell apoptosis; qRT-PCR and Western blot assays were performed to evaluate the effect of corilagin on the expression levels of Bax, Bcl-2, Caspase-3, Cleaved Caspase-3 in CAL-27 cells. In vivo experiments, tumor-bearing nude mice was constructed with CAL-27 cells to evaluate the antitumor effect of corilagin. GraphPad Prism 8.0 software package was used for statistical analysis of the data. RESULTS: In vitro experiments showed that corilagin in a dose-dependent manner inhibited proliferation, induced apoptosis, up-regulated Bax, caspase-3, cleaved caspase-3 and down-regulated Bcl-2 at the mRNA and protein levels of CAL-27 cells, and the differences were statistically significant(P<0.05). In vivo experiments showed that compared with the control group, corilagin could significantly reduce the volume of tumor in nude mice(P<0.05). CONCLUSIONS: Corilagin can significantly inhibit CAL-27 cell growth and promote its apoptosis both in vitro and in vivo, which may be related to the mediation of Bax/Bcl-2/Caspase-3 signaling pathway.

Key words: Corilagin, CAL-27 cells, Proliferation, Apoptosis, Bax/Bcl-2/Caspase-3 signal pathway

中图分类号:

R739.8

李胜男, 崔飞艳, 赵姗, 杜琛, 孟箭. 柯里拉京体内外诱导口腔鳞癌CAL-27细胞凋亡及机制探讨[J]. 上海口腔医学, 2023, 32(5): 462-467.

LI Sheng-nan, CUI Fei-yan, ZHAO Shan, DU Chen, MENG Jian. Corilagin-induced apoptosis of oral squamous carcinoma CAL-27 cells in vitro and in vivo and its mechanism[J]. Shanghai Journal of Stomatology, 2023, 32(5): 462-467.

参考文献

[1] Lu Y, Zheng Z, Yuan Y, et al.The emerging role of exosomes in oral squamous cell carcinoma[J]. Front Cell Dev Biol, 2021, 9: 628103.
[2] Sung H, Ferlay J, Siegel RL, et al.Global cancer statistics 2020: globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249.
[3] Weiβe J, Rosemann J, Krauspe V, et al.RNA-binding proteins as regulators of igration, invasion and metastasis in oral squamous cell carcinoma[J]. Int J Mol Sci, 2020, 21(18): 6835-6863.
[4] Rajasekaran S, Rajasekar N, Sivanantham A.Therapeutic potential of plant-derived tannins in non-malignant respiratory diseases[J]. J Nutr Biochem, 2021, 94: 108632.
[5] 刘转转, 徐娜, 高瑞芳, 等. 黄芩苷调控的IKKα对HOKs炎症反应的保护作用[J]. 上海口腔医学, 2021, 30(6): 589-594.
[6] Gupta A, Singh AK, Kumar R, et al.Corilagin in cancer: a critical evaluation of anticancer activities and molecular mechanisms[J]. Molecules, 2019, 24(18): 3399-3418.
[7] Tang YY, He XM, Sun J, et al.Polyphenols and alkaloids in byproducts of longanfruits (dimocarpus longan lour.) and their bioactivities[J]. Molecules, 2019, 24(6): 1186-1201.
[8] Jin F, Cheng D, Tao JY, et al.Anti-inflammatory and anti-oxidative effects of corilagin in a rat model of acute cholestasis[J]. BMC Gastroenterol, 2013, 13: 79-89.
[9] Liu FC, Chaudry IH, Yu HP.Hepatoprotective effects of corilagin following hemorrhagic shock are through Akt-dependent pathway[J]. Shock, 2017, 47(3): 346-351.
[10] Wu C, Huang H, Choi HY, et al.Anti-esophageal cancer effect of corilagin extracted from phmllanthi fructus via the mitochondrial and endoplasmic reticulum stress pathways[J]. J Ethnopharmacol, 2021, 269: 113700.
[11] Yu X, Wu T, Liao B, et al.Anticancer potential of corilagin on T24 and TSGH 8301 bladder cancer cells via the activation of apoptosis by the suppression of NF-κB-induced PI3K/Akt signaling pathway[J]. Environ Toxicol, 2022, 37(5): 1152-1159.
[12] Zhang L, Jia B, Velu P, et al.Corilagin induces apoptosis and inhibits HMBG1/PI3K/AKT signaling pathways in a rat model of gastric carcinogenesis induced by methylnitronitrosoguanidine[J]. Environ Toxicol, 2022, 37(5): 1222-1230.
[13] Ke H, Wang X, Zhou Z, et al.Effect of weimaining on apoptosis and Caspase-3 expression in a breast cancer mouse model[J]. J Ethnopharmacol, 2021, 264:113363.
[14] Lang L, Zhu S, Zhang H, et al.A natural phenylpropionate derivative from Mirabilis himalaica inhibits cell proliferation and induces apoptosis in HepG2 cells[J]. Bioorg Med Chem Lett, 2014, 24(23): 5484-5488.
[15] Omori H, Nishio M, Masuda M, et al. YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma[J]. Sci Adv, 2020, 6(12): eaay3324.
[16] Thomson PJ.Perspectives on oral squamous cell carcinoma prevention-proliferation, position, progression and prediction[J]. J Oral Pathol Med, 2018, 47(9): 803-807.
[17] Ren G, Ju H, Wu Y, et al.A multicenter randomized phase Ⅱ trial of hyperthermia combined with TPF induction chemotherapy compared with TPF induction chemotherapy in locally advanced resectable oral squamous cell carcinoma[J]. Int J Hyperthermia, 2021, 38(1): 939-947.
[18] Vermorken JB, Specenier P.Optimal treatment for recurrent/metastatic head and neck cancer[J]. Ann Oncol, 2010, 21(Suppl 7): 252-261.
[19] 尹可欢, 罗晓敏, 丁翼, 等. 余甘子及其活性成分肝保护作用及机制的研究进展[J]. 中草药, 2022, 53(1): 295-307.
[20] Hau DK, Gambari R, Wong RS, et al.Phyllanthus urinaria extract attenuates acetaminophen induced hepatotoxicity: involvement of cytochrome P450 CYP2E1[J]. Phytomedicine, 2009, 16(8): 751-760.
[21] Li X, Deng Y, Zheng Z, et al.Corilagin, a promising medicinal herbal agent[J]. Biomed Pharmacother, 2018, 99(1): 43-50.
[22] Xu J, Zhang G, Tong Y, et al.Corilagin induces apoptosis, autophagy and ROS generation in gastric cancer cells in vitro[J]. Int J Mol Med, 2019, 43(2): 967-979.
[23] Qiu F, Liu L, Lin Y, et al.Corilagin inhibits esophageal squamous cell carcinomaby inducing DNA damage and down-regulation of RNF8[J]. Anticancer Agent Med Chem, 2019, 19(8): 1021-1028.
[24] Jia S, He D, Liang X, et al.Corilagin induces apoptosis and inhibits autophagy of HL-60 cells by regulating miR-451/HMGB1 axis[J]. Mol Med Rep, 2022, 25(1): 34-42.
[25] Xu X, Lai Y, Hua ZC. Apoptosis and apoptotic body: disease message and therapeutic target potentials[J]. Biosci Rep, 2019, 39(1): BSR20180992.
[26] Pistritto G, Trisciuoglio D, Ceci C, et al.Apoptosis as anticancer mechanism: function and dysfunction of its modulators and targeted therapeutic, strategies[J]. Aging (Albany NY), 2016, 8(4): 603-619.
[27] Li J, Yuan J.Caspases in apoptosis and beyond[J]. Oncogene, 2008, 27(48): 6194-6206.
[28] Gong R, Wang D, Abbas G, et al.A switch-on molecular biosensor for detection of Caspase-3 and imaging of apoptosis of cells[J]. Sci China Life Sci, 2022, 65(3): 540-549.
[29] Brentnall M, Rodriguez-Menocal L, De Guevara RL, et al.Caspase-9, Caspase-3 and Caspase-7 have distinct roles during intrinsic apoptosis[J]. BMC Cell Biol, 2013, 14: 32-40.

柯里拉京体内外诱导口腔鳞癌CAL-27细胞凋亡及机制探讨

Corilagin-induced apoptosis of oral squamous carcinoma CAL-27 cells in vitro and in vivo and its mechanism

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘文静, 李梦琦, 崔祥, 汪俊兰. circ_0000326靶向miR-567对口腔鳞状细胞癌HSC3细胞增殖、侵袭及迁移的调控效应[J]. 上海口腔医学, 2023, 32(6): 590-596.
[2]	刘岩, 单丹妮, 孙晶, 邹雨希, 袁长永. Let-7a对人牙髓干细胞增殖、凋亡和成骨分化的影响[J]. 上海口腔医学, 2023, 32(5): 468-474.
[3]	迪丽达尔·塔西甫拉提, 牛雅琪, 热依沙·阿布都克依木, 王玲. 长链非编码RNA AWPPH通过调控Notch信号通路对人牙周膜细胞增殖和成骨向分化的影响[J]. 上海口腔医学, 2023, 32(1): 23-27.
[4]	魏军水, 孙鑫, 徐金标, 陈逸达. LncRNA RUNX1-IT1对恶性多形性腺瘤miR-195/CyclinD1的调控作用探讨[J]. 上海口腔医学, 2023, 32(1): 85-90.
[5]	杨恩黎, 王娜, 李名扬, 李易铭, 耿一鸣, 张东升, 吴海威. 紫草素对血管瘤内皮细胞增殖、凋亡、迁移及成血管的影响[J]. 上海口腔医学, 2022, 31(6): 576-580.
[6]	袁尉力, 王绪凯. 口服普萘洛尔对增殖期血管瘤患儿尿液bFGF、MMP-2和MMP-9表达水平的影响[J]. 上海口腔医学, 2022, 31(4): 400-405.
[7]	秦志明, 揭伟萍, 池彦廷, 李宏锋, 李斌斌. 基于局部可变阈值的无监督定量Ki-67细胞增殖指数模型的初步建立[J]. 上海口腔医学, 2022, 31(3): 248-254.
[8]	陈祺, 谢非, 季颖颖, 魏魁杰, 任强. LLY-283靶向PRMT5抑制头颈部鳞癌增殖和转移的实验研究[J]. 上海口腔医学, 2022, 31(2): 142-147.
[9]	马曌磊, 刘佳楠, 苏荣健, 贺武斌, 黄克强. Met激酶抑制剂BMS-777607对舌鳞癌细胞CAL27增殖、凋亡的影响[J]. 上海口腔医学, 2021, 30(6): 573-578.
[10]	汤颖, 沈忆芬, 刘超, 邱吟枫, 顾永春, 于金华. 高浓度氟对人牙周膜干细胞凋亡的影响[J]. 上海口腔医学, 2021, 30(4): 360-366.
[11]	马春燕, 潘清, 何磊, 杨海兵. 细胞外基质PDMS硬度对牙髓干细胞增殖和成骨分化的影响[J]. 上海口腔医学, 2021, 30(3): 253-257.
[12]	王科, 彭国光, 谭玉莲, 何善志, 罗翠芬. miR-99a对口腔鳞癌增殖能力的影响[J]. 上海口腔医学, 2021, 30(1): 44-49.
[13]	王松松, 张云涛, 刘珂珂, 张凌楠, 段昕. 高度浓缩生长因子对MC3T3-E1成骨细胞生物学性能的影响[J]. 上海口腔医学, 2020, 29(5): 482-486.
[14]	郑晶, 李天客, 包阳, 张素欣. Spindly和Bub3在口腔鳞状细胞癌中的表达及意义[J]. 上海口腔医学, 2020, 29(5): 528-532.
[15]	田一弘, 刘美希, 张强, 夏晨蕾, 阎潇, 袁晓. 周期性机械应力诱导的自噬抑制caspase通路依赖的成肌细胞凋亡[J]. 上海口腔医学, 2020, 29(3): 231-236.