大蒜素对肥胖伴牙周炎大鼠胰岛素抵抗及游离脂肪酸水平的影响

doi:10.19439/j.sjos.2023.04.007

摘要/Abstract

摘要： 目的探讨大蒜素对肥胖伴牙周炎大鼠胰岛素抵抗及游离脂肪酸（free fatty acid,FFAs）水平的影响。方法 40只大鼠随机分为健康组、牙周炎组及低、中、高剂量组,每组8只。健康组为健康大鼠,其余各组均以谷氨酸钠（MSG）诱法,成功建立肥胖模型后,结扎上颌磨牙并涂菌,建立牙周炎模型。牙周炎组和健康组均给予生理盐水,低、中、高剂量组分别给予大蒜素20、40、60 mg·kg^-1·d^-1,灌胃给药。治疗21天后,检测大鼠空腹血糖（FPG）、空腹胰岛素（FINS）,稳态模型的胰岛素抵抗指数（HOMA-IR）评分及FFAs水平,比较TLR4/MyD88信号通路的蛋白表达。采用SPSS 22.0软件包对数据进行统计学分析。结果与健康组相比,牙周炎组的FPG、FINS水平,HOMA-IR,IL-6及TNF-α水平显著升高,TLR4、MyD88蛋白表达显著上调（P<0.05）。与牙周炎组相比,低、中、高剂量的FPG、FINS水平,HOMA-IR,IL-6及TNF-α水平显著降低,TLR4、MyD88蛋白表达显著下调（P<0.05）。与低剂量组相比,中、高剂量组FPG、FINS水平,HOMA-IR,IL-6及TNF-α水平显著降低,TLR4、MyD88蛋白表达显著下调（P<0.05）。与中剂量组相比,高剂量组FPG、FINS水平,HOMA-IR,IL-6及TNF-α水平显著降低,TLR4、MyD88蛋白表达显著下调（P<0.05）。低、中、高剂量组治疗后的FFAs显著低于治疗前（P<0.05）。治疗后,与健康组相比,牙周炎组、低、中剂量组FFAs水平显著升高。与牙周炎组相比,低、中、高剂量组FFAs水平显著降低;与低剂量组相比,高剂量组FFAs水平显著降低;与中剂量组相比,高剂量组FFAs水平显著降低（P<0.05）。结论大蒜素能改善肥胖伴牙周炎大鼠胰岛素抵抗和肥胖情况,其作用机制与TLR4/MyD88信号通路有关。

关键词: 大蒜素, 肥胖, 牙周炎, 胰岛素抵抗, 游离脂肪酸, TLR4/MyD88信号通路

Abstract: PURPOSE: To explore the effects of allicin on insulin resistance and free fatty acids (FFAs) levels in obese rats with periodontitis. METHODS: Forty rats were randomly divided into healthy group, periodontitis group, and low, medium and high dose groups, with 8 rats in each group. The healthy group was healthy rats, and the other groups were induced by sodium glutamate(MSG). After successfully establishing an obesity model, the maxillary molars were ligated and smeared to establish a periodontitis model. Both the periodontitis group and the healthy group were given normal saline, and the allicin low, medium and high dose groups were given allicin 20, 40 and 60 mg·kg^-1·d^-1, mixed with feed for oral administration. After 21 days of treatment, the fasting blood glucose(FPG), fasting insulin (FINS), insulin resistance index (HOMA-IR) scores and FFAs levels of the homeostatic model in rats were detected. The protein expression of TLR4/MyD88 signaling pathway were compared. Statistical analysis was performed with SPSS 22.0 software package. RESULTS: Compared with the healthy group, FPG, FINS levels, HOMA-IR, IL-6 and TNF-α levels of the periodontitis group were significantly increased, and the expression of TLR4 and MyD88 proteins was significantly increased(P<0.05). Compared with the periodontitis group, FPG, FINS levels, HOMA-IR, IL-6 and TNF-α levels of low, medium and high-doses groups were significantly decreased, and the expression of TLR4 and MyD88 proteins was significantly decreased (P<0.05). Compared with the low-dose group, the levels of FPG and FINS, HOMA-IR, IL-6 and TNF-α levels of the middle and high-dose groups were significantly decreased, and the expression of TLR4 and MyD88 proteins was significantly decreased (P<0.05). Compared with the middle-dose group, the levels of FPG and FINS, HOMA-IR, IL-6 and TNF-α levels of the high-dose group were significantly decreased, and the expression of TLR4 and MyD88 proteins was significantly decreased (P<0.05). After treatment, FFAs of the low, medium and high-dose groups were significantly lower than those before treatment(P<0.05). Compared with the healthy group, FFAs levels of the periodontitis group, low-dose and medium-dose groups were significantly increased. Compared with the periodontitis group, FFAs levels of the low, medium and high-dose groups were significantly increased. Compared with the low-dose group, FFAs levels of the high-dose group were significantly increased. Compared with the middle-dose group, FFAs levels of the high-dose group were significantly increased (P<0.05). CONCLUSIONS: Allicin can improve insulin resistance and obesity in obese rats with periodontitis, and its mechanism of action is related to the TLR4/MyD88 signaling pathway.

Key words: Allicin, Obesity, Periodontitis, Insulin resistance, Free fatty acid, TLR4/MyD88 signaling pathway

中图分类号:

R781.4

谢兰芬, 李晓静, 黄晓霖, 赵升柯. 大蒜素对肥胖伴牙周炎大鼠胰岛素抵抗及游离脂肪酸水平的影响[J]. 上海口腔医学, 2023, 32(4): 375-379.

XIE Lan-fen, LI Xiao-jing, HUANG Xiao-lin, ZHAO Sheng-ke. Effects of allicin on insulin resistance and free fatty acid levels in obese rats with periodontitis[J]. Shanghai Journal of Stomatology, 2023, 32(4): 375-379.

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