黄芩苷调控的IKKα对HOKs炎症反应的保护作用

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (6): 589-594.doi: 10.19439/j.sjos.2021.06.006

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黄芩苷调控的IKKα对HOKs炎症反应的保护作用

刘转转, 徐娜, 高瑞芳, 张芳

山西医科大学口腔医学院,口腔疾病防治与新材料山西省重点实验室,山西太原 030001

收稿日期:2020-03-23 修回日期:2020-05-13 发布日期:2022-03-09
通讯作者: 张芳,E-mail: doctor-fangzh@hotmail.com
作者简介:刘转转（1993-）,女,在读硕士研究生,E-mail:1130404311@qq.com
基金资助:
山西省应用基础研究计划青年基金（02201522）; 山西医科大学附属口腔医院基金（KY201706）; 山西医科大学博士启动基金（14055245）

Protection effect of baicalin-regulated IKKα on inflammatory reaction of human oral keratinocytes

LIU Zhuan-zhuan, XU Na, GAO Rui-fang, ZHANG Fang

School of Stomatology, Shanxi Medical University, The Key Laboratory of Oral Disease Prevention and New Materials of Shanxi Province. Taiyuan 030001, Shanxi Province, China

Received:2020-03-23 Revised:2020-05-13 Published:2022-03-09

摘要/Abstract

摘要： 目的: 观察黄芩苷对HOKs炎症反应中IKKα介导的MASPIN的调控作用,探讨黄芩苷对口腔黏膜炎症的分子调控机制。方法: 以脂多糖（LPS）刺激人口腔角质细胞（human oral keratinocytes, HOKs）,体外局部模拟口腔黏膜上皮细胞炎性环境。CCK-8法检测黄芩苷对HOKs的毒性作用;在LPS刺激的HOKs中预先加入不同浓度黄芩苷,应用ELISA法检测黄芩苷对LPS刺激的HOKs中IL-6和TNF-α分泌的调控作用;利用RT-PCR及Western免疫印迹检测黄芩苷对LPS刺激的HOKs中IKKα介导的MASPIN基因及蛋白表达水平的调控作用。采用SPSS 21.0软件包对数据进行统计学分析。结果: 以10 μg/mLLPS刺激HOKs,可作为口腔黏膜上皮细胞炎症模型。黄芩苷（1~20 μg/mL）对HOKs无毒性作用;随着黄芩苷浓度的升高,MASPIN在LPS刺激的HOKs中的表达逐渐升高,而IKKα及炎性细胞因子TNF-α、IL-6的表达逐渐降低（P<0.05）。结论: 在LPS刺激的HOKs中,黄芩苷可降低炎性因子表达,下调IKKα,上调MASPIN。

关键词: 黄芩苷, 乳腺丝氨酸蛋白酶抑制剂, IKKα, 口腔黏膜炎症

Abstract: PURPOSE: To observe the regulation of baicalin on IKKα mediated MASPIN in Human oral keratinocytes (HOKs) inflammatory reaction, this study was to explore the molecular regulation mechanism of baicalin on oral mucosal inflammation. METHODS: HOKs were stimulated by lipopolysaccharide (LPS) to mimic the inflammatory response of oral mucosal inflammation in vitro. CCK-8 assay was used to detect the toxicity of baicalin to HOKs; then different concentrations of baicalin were pre-treated to LPS-stimulated HOKs, enzyme-linked immunosorbent assays (ELISA) was used to detect the secretion of IL-6 and TNF-α in LPS-stimulated HOKs; reverse transcription polymerase chain reaction(RT-PCR) and Western blot assay were used to detect the regulatory effects of baicalin on gene and protein expression levels of IKKα mediated MASPIN in LPS-stimulated HOKs. SPSS 21.0 software package was used for statistical analysis of the data. RESULTS: HOKs stimulated by 10 μg/mL LPS successfully simulated the inflammatory environment of oral mucosal inflammation. The concentration of baicalin between 1 μg/mL and 20 μg/mL had no toxic effect on HOKs. With the increasing concentration of baicalin, the expression of MASPIN increased gradually, while the expression of IKKα and inflammatory factors decreased gradually(P<0.05). CONCLUSIONS: Baicalin can decrease the expression of inflammatory factors in LPS-stimulated HOKs, down-regulate IKKα and up-regulate MASPIN.

Key words: Baicalin, Mammary serine protease inhibitor, IKKα, Oral mucosal inflammation

中图分类号:

R781.5

刘转转, 徐娜, 高瑞芳, 张芳. 黄芩苷调控的IKKα对HOKs炎症反应的保护作用[J]. 上海口腔医学, 2021, 30(6): 589-594.

LIU Zhuan-zhuan, XU Na, GAO Rui-fang, ZHANG Fang. Protection effect of baicalin-regulated IKKα on inflammatory reaction of human oral keratinocytes[J]. Shanghai Journal of Stomatology, 2021, 30(6): 589-594.

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