全口义齿修复对口腔微生态影响的初步探讨

doi:10.19439/j.sjos.2018.01.013

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (1): 56-60.doi: 10.19439/j.sjos.2018.01.013

全口义齿修复对口腔微生态影响的初步探讨

于卫强¹, 田菲², 郭晓奎², 张富强¹, 叶冬霞³

1.上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科,上海 200011
2.上海交通大学医学院病原生物学教研室,上海 200025
3.上海市口腔医学研究所,上海 200011

收稿日期:2017-04-13 修回日期:2017-08-15 出版日期:2018-02-25 发布日期:2018-03-05
通讯作者: 叶冬霞,E-mail: yedongxia122@hotmail.com
作者简介:于卫强（1985-）,男,主治医师,E-mail:ywqiang@126.com
基金资助:
上海市卫生局项目 (2012173)

Changes of oral bacteria flora after wearing complete denture

YU Wei-qiang¹, TIAN Fei², GUO Xiao-kui², ZHANG Fu-qiang¹, YE Dong-xia³

1.Department of Prosthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011
2.Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine. Shanghai 200025
3. Shanghai Research Institute of Stomatology. Shanghai, 200011, China

Received:2017-04-13 Revised:2017-08-15 Online:2018-02-25 Published:2018-03-05

摘要/Abstract

摘要： 目的: 初步探讨全口义齿修复对口腔微生态的影响。方法: 选取11例全口义齿修复后7 d的患者,收集口腔不同软组织位点、唾液及义齿表面的菌斑,构建细菌16S rRNA基因文库。采用焦磷酸测序技术针对细菌的16S rRNA基因序列的V2-V3可变区进行序列分析,完成口腔菌群的物种鉴定。结果: 11例全口义齿修复患者共测得64800条序列,其中37416条序列为缓症链球菌、溶血孪生球菌、黏液罗氏菌、卟啉单胞菌、动物咬伤奈瑟球菌、苛养颗粒链菌、鲍曼不动杆菌、香茅醇假单胞菌、毗邻颗粒链菌和梭杆菌,为口腔优势菌种。全口义齿组织面的物种与唇颊前庭区和口底菌种较为相似,而全口义齿光滑面的物种与口底及舌腹区黏膜菌种的相似度较高。结论: 全口义齿修复对口腔菌群的影响仍有一定局限性,口腔菌群组成不同还受到个体差异等多因素影响。

关键词: 全口义齿, 口腔微生态, 16S rRNA

Abstract: PURPOSE: This study investigateed the influence of complete denture on oral bacteria flora. METHODS: Bateria plaque samples in oral mucosa, saliva and denture surfaces in 11 edentulous patients were collected, then the 16S rRNA gene clone libraries were constructed. Pyrosequencing was used to analyze the 16S rRNA gene V3~V4 regions, oral bacteria flora were classified and identified. RESULTS: There were 64800 sequences in complete denture-wearing subjects, Streptococcus mitis, Gemella haemolysans, Rothia mucilaginosa, Porphyromonas sp, Neisseria zoodegmatis, Granulicatella elegans, Acinetobacter baumannii, Pseudomonas citrinum, Granulicatella adiacens and Fusobacterium canifelinum were the predominant species (37416 sequences). The species of denture tissue surface were similar to these of buccal vestibule after wearing denture, and the species of denture smooth surface were similar to these of tongue ventrum and the floor of mouth. CONCLUSIONS: The effect of complete denture on oral flora is still limited, and the composition of oral flora is influenced by many other factors.

Key words: Complete denture, Oral bacteria flora, 16S rRNA

中图分类号:

R783

于卫强, 田菲, 郭晓奎, 张富强, 叶冬霞. 全口义齿修复对口腔微生态影响的初步探讨[J]. 上海口腔医学, 2018, 27(1): 56-60.

YU Wei-qiang, TIAN Fei, GUO Xiao-kui, ZHANG Fu-qiang, YE Dong-xia. Changes of oral bacteria flora after wearing complete denture[J]. Shanghai Journal of Stomatology, 2018, 27(1): 56-60.

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全口义齿修复对口腔微生态影响的初步探讨

Changes of oral bacteria flora after wearing complete denture

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