上海口腔医学

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (1): 48-53.doi: 10.19439/j.sjos.2017.01.010

• 论著 • 上一篇    下一篇

氟化钠对人牙周膜细胞增殖及矿化能力的影响

李坤芩1, 2, 徐乐1, 2, 黄圣运3, 张东升1, 3   

  1. 1.山东大学口腔医学院,山东 济南 250012;
    2.山东省口腔组织再生重点实验室,山东 济南 250012;
    3.山东大学附属省立医院 口腔科,山东 济南 250021
  • 收稿日期:2016-07-05 修回日期:2016-09-08 出版日期:2017-02-25 发布日期:2017-03-20
  • 通讯作者: 张东升,E-mail: ds63zhang@sdu.edu.cn
  • 作者简介:李坤芩（1991-）,女,硕士,E-mail：xingqin9510@163.com

Effect of NaF on proliferation and mineralization of human periodontal ligament cells

LI kun-qin1, 2, XU Le1, 2, HUANG Sheng-yun3, ZHANG dong-sheng1, 3   

  1. 1.School of Stomatology of Shandong University. Jinan 250012;
    2.Shandong Provincial Key Laboratory of Oral Tissue Regeneration. Jinan 250012;
    3.Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong University. Jinan 250021, Shandong Province, China
  • Received:2016-07-05 Revised:2016-09-08 Online:2017-02-25 Published:2017-03-20

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摘要： 目的 观察氟化钠对体外培养的人牙周膜细胞增殖及矿化能力的影响,为氟添加入牙周组织工程药物中的应用提供依据。方法 原代培养并鉴定人牙周膜细胞,应用CCK8检测不同浓度NaF对hPDLCs增殖的影响,并筛选出4个浓度用于矿化实验。矿化条件下,将0、 1×10-5、 5×10-4 和1×10-3 mol/L的NaF作用hPDLCs后,通过碱性磷酸酶（ALP）染色、茜素红染色和实时荧光定量PCR检测矿化能力及成骨相关基因的表达。采用 SPSS20.0 软件包对数据进行单因素方差分析。结果 5×10-5、1×10-4、 5×10-4 mol/L的NaF均能促进hPDLCs 增殖,且以5×10-4 mol/L效果最佳（P<0.05）。而1×10-5 mol/L的NaF碱性磷酸酶染色阳性面积最大、茜素红染色矿化结节数量最多（P<0.05）。RT-PCR结果根据时间、指标变化程度较大。结论 5×10-5、1×10-4、5×10-4 mol/L的NaF能促进hPDLCs 的增殖能力, 1×10-5 mol/L的NaF能提高hPDLCs的碱性磷酸酶活性及钙结节形成。

关键词: 氟, 牙周膜细胞, 矿化, 牙周炎

Abstract: PURPOSE: To investigate the effect of NaF on proliferation and mineralization of human periodontal ligament cells (hPDLCs). METHODS: hPDLCs were isolated and characterized. The proliferation of hPDLCs treated with different concentration of NaF was tested by CCK-8. Four moderate concentrations were chosen for subsequent experiments. The mineralization was investigated using ALP activity assay, Alizarin red S staining and quantitative real-time polymerase chain reaction(RT-PCR). The data were statistically analyzed with SPSS20.0 software package. RESULTS: Immunohistochemistry showed that the isolated cells were hPDLCs. 5×10-5, 1×10-4 and 5×10-4 mol/L NaF had pro-proliferation effects while 5×10-4 mol/L resulted in the best effect (P<0.05). ALP activity and calcium content was significantly enhanced by 1×10-5 mol/L NaF with osteogenic inductive medium (P<0.05). Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. CONCLUSIONS: 5×10-5, 1×10-4, 5×10-4 mol/L NaF can stimulate proliferation in hPDLCs, 1×10-5 mol/L NaF can enhance ALP activity and calcium content formation of hPDLCs.

Key words: Fluoride, Periodontal ligament cells, Mineralization, Periodontitis

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