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    25 February 2018, Volume 27 Issue 1 Previous Issue    Next Issue
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    Orginal Articles
    Effects of Porphyromonas endodontalis lipopolysaccharides on the expression of monocyte chemotactic protein-1 in mouse osteoblasts
    LI Xiao-lin, YU Ya-qiong, QIU Li-hong, GUO Jia-jie, SHAO Li-na, WANG Si-mo, YANG Di
    2018, 27 (1):  1-5.  doi: 10.19439/j.sjos.2018.01.001
    Abstract ( 306 )   PDF (3643KB) ( 116 )  
    PURPOSE: To investigate the effects of lipopolysaccharide (LPS) extracted from Porphyromonas endodontalis (P.endodontalis) on expression of monocyte chemotactic protein-1 (MCP-1) mRNA and protein in MC3T3-E1 cells and the role of p38 mitogen-activated protein kinase (MAPK) and nuclear factor-κB(NF-κB)in the process. METHODS: MC3T3-E1 cells were treated with different concentrations of P.endodontalis LPS(0-50mg/L) and 20 mg/L P.endodontalis LPS for different hours (0-48 h). The expression of MCP-1 mRNA was detected by real-time reverse transcription PCR(RT-PCR) and protein was detected by enzyme-1inked immunosorbent assay (ELISA). MC3T3-E1 cells were pretreated with SB203580 (inhibitor of p38MAPK) and BAY11-7082 (inhibitor of NF-κB) for 1h, and then were treated with 20 mg/L P.endodontalis LPS for 24 h, the expression of MCP-1 mRNA was also detected by RT-PCR. Statistical analysis was performed using one-way ANOVA and Dunnett t test with SPSS 13.0 software package. RESULTS: The level of MCP-1 mRNA and protein increased significantly after treatment with different concentrations of P.endodontalis LPS (0-50 mg/L), which indicated that P.endodontalis LPS induced osteoblasts to express MCP-1 in a dose dependent manners. During the observation time (0-48 h), the impact of 20 mg/L P.endodontalis LPS on induction of MCP-1 in MC3T3-E1 cells exhibited a time-dependent manner. The expression of MCP-1 mRNA decreased significantly after pretreated with 10 mol/L SB203580 and BAY11-7082 for 1 h,and the inhibitory effect of SB203580 was stronger than BAY11-7082. CONCLUSIONS: P.endodontalis LPS may induce the expression of MCP-1 mRNA and protein in MC3T3-E1 cells through the signaling pathway of p38MAPK and NF-κB.
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    Micro-CT study of root canal morphology of mandibular first premolars with multi-canals
    SUN Chu-wen, LIU Yu, YANG Yi-ming, LIAO Qian
    2018, 27 (1):  6-10.  doi: 10.19439/j.sjos.2018.01.002
    Abstract ( 401 )   PDF (3762KB) ( 233 )  
    PURPOSE: To observe root canal system of multi-canal mandibular first premolars and to analyze the morphology of them by micro-CT and three-dimensional reconstruction. METHODS: Thirty-two extracted mandibular first premolars with multiple root canals were selected and scanned by micro-CT. The number and morphology of canals, location of bifurcation and 5 subtle structures (lateral canals, apical ramification, isthmus, loops, apical delta) were analyzed. RESULTS: In the 32 specimens, 96.88% contained radicular depressions or grooves. The canal configuration was type Ⅱ, Ⅲ, Ⅴ: 3.12%, type Ⅳ: 31.25%, type Ⅷ: 9.38%, type Ⅸ: 15.62% and C-shaped canal: 34.38% by Vertucci's classification. 62.50% of the canals bifurcated at the middle third of roots, comparing with 37.50% at apical third. The incidence of the lateral canals, apical ramification, isthmus, loops and apical delta was 56.25%, 43.75%, 40.62%, 21.88% and 6.25%, respectively, which were all found at the middle and apical third of roots except that lateral canals were found at all depth of canals. CONCLUSIONS: Most mandibular first premolars with multiple canals contain radicular depression or grooves, of which root canal system are complicated. Micro-CT can provide clear and accurate three-dimensional information of canal morphology.
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    MicroRNA-125b regulates osteogenic differentiation of human periodontal ligament stem cells
    DU Li, CAO Wei-jing, TIAN Ying, WANG Yuan-ming
    2018, 27 (1):  11-17.  doi: 10.19439/j.sjos.2018.01.003
    Abstract ( 197 )   PDF (4970KB) ( 222 )  
    PURPOSE: To investigate the effect of microRNA-125b (miR-125b) on osteogenic differentiation of periodontal ligament stem cell (PDLSCs). METHODS: The surface factor of isolated PDLSCs was detected by flow cytometry. After transfected with miR-125b or anti-miR125b in PDLSCs, MTT and LDH were used to detect cell viability and cytotoxicity; ALP activity and calcium level were detected by ALP assay kit and calcium colorimetric assay kit. Western blot and RT-qPCR were used to determine the expression of osteogenesis-related genes. The interaction between miR-125b and connexin 43 (Cx43) was detected by dual luciferase gene reporter assay. The data were analyzed with SPSS 17.0 software. RESULTS: The cultured PDLSCs showed the characteristics of mesenchymal stem cells. After transfected with miR-125b in PDLSCs, the cell viability was decreased, cytotoxicity was increased; ALP activity, calcium level and the expression of osteogenesis-related genes were significantly decreased. On the contrary, cell viability, ALP activity, calcium level and the expression of osteogenesis-related genes in PDLSCs were increased after anti-miR-125b transfection. Dual luciferase gene reporter assay showed that miR-125b could target Cx43. In addition, the effect of miR-125b on osteogenic differentiation of PDLSCs was reversed after Cx43 overexpression. CONCLUSIONS: miR-125b may inhibit osteogenic differentiation of PDLSCs by targeting Cx43.
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    Expression of vascular endothelial growth factor in periodontal tissues during orthodontic tooth movement and its role in bone remodeling
    YUE Yang, CHEN Zhuo, XIE Bing, YAO Hai-liang, ZHANG Xian, YAN Li-hui, ZHANG Zhi-hua
    2018, 27 (1):  18-21.  doi: 10.19439/j.sjos.2018.01.004
    Abstract ( 280 )   PDF (3030KB) ( 159 )  
    PURPOSE: To investigate the expression of VEGF in periodontal tissues and its role in bone remodeling during orthodontic tooth movement in rats. METHODS: Rat model of tooth movement was established, and 30 rats in the experimental group were divided into 5 groups, 6 rats in each group. At 2 days, 4 days, 7 days, 14 days and 20 days, the rats were sacrificed to make periodontal tissue slice. The morphological changes of periodontal tissue were observed under H-E staining, and the expression of VEGF was semi-quantitatively analyzed by immunohistochemistry. The data were analyzed using SPSS21.0 software package. RESULTS: Expression of VEGF in normal rat periodontal tissues was very weak or without positive expression; 2 days after tooth movement, VEGF expression in periodontal tissues began to increase from seventh to fourteenth days; on the fourteenth day, the expression reached a peak; after twentieth day, the expression of VEGF decreased slightly, but still higher than the control group. The expression of VEGF was mainly found in the cytoplasm of vascular endothelial cells, osteoblasts, fibroblasts, and osteoclasts. CONCLUSIONS: Expression of VEGF may play an important role in the early remodeling of periodontal tissues during orthodontic tooth movement.
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    Effect of overdose fluoride on the expression of TGF-β3 in rat incisor
    LUO Ping-ping, XU Hua-shun
    2018, 27 (1):  22-24.  doi: 10.19439/j.sjos.2018.01.005
    Abstract ( 237 )   PDF (2275KB) ( 178 )  
    PURPOSE: To observe the effect of overdose fluoride on the expression of TGF-β3 in rat incisor and to explore the possible mechanism of dental fluorosis. METHODS: Twenty Wistar rats were randomly divided into 2 groups. The animals were maintained in standard environmental conditions with free access to food and water (control group) or water added with 100mg/L F (experimental group). The rats were killed at the end of 8th week. The expression of TGF-β3 was detected by immunohistochemical staining. Statistical analysis was performed using SPSS12.0 software package. RESULTS: The expression of TGF-β3 in ameloblasts was significantly inhibited in the experimental of group Ⅱ(P<0.01). The gray value of the control group and the fluorine group were 85.89±7.90, 116.76±8.04, respectively. CONCLUSIONS: Fluoride might disturb the signal transduction between the epithelia and mesenchyma by inhibiting the expression of TGF-β3 in ameloblasts, which in turn may inhibit the differentiation and function of the tooth-forming cells.
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    Influences of filler content and size on the translucency parameter of resin composites
    QIAN Cheng-hui, WANG Jue
    2018, 27 (1):  25-27.  doi: 10.19439/j.sjos.2018.01.006
    Abstract ( 209 )   PDF (2196KB) ( 309 )  
    PURPOSE: The objective of this study was to investigate the influences of filler content and size on the translucency parameter of non-layered resin composites. METHODS: Three types of fillers (0.7 μm, 1.0 μm and 1.5 μm) were mixed at weight contents of 60wt%, 70wt%, 75wt%, and 80wt%. The L*, a* and b* value was analyzed based on CIELab color space of the specimen respectively on black and white background. The results of each test were analyzed with one-way ANOVA followed by Tukey's HSD test using SPSS 20.0 software package. RESULTS: For each filler size, there was a trend of decreasing translucency parameter as the filler content increased (P<0.05). The filler size did not affect the translucency parameter of the composites in this study. CONCLUSIONS: When the fillers content changed from 60wt% to 80wt%, there is a trend of decreasing translucency parameter as the filler content increased, and the filler size may not influence the translucency parameter of the composites when the size changed from 0.7 μm to 1.5 μm.
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    KLF5 modulates proliferation and osteogenic differentiation of human periodontal ligament cells subjected to cyclic tensile stress
    GAO Jin-yu, YU Xiao-qing, WANG Jun-qiang
    2018, 27 (1):  28-33.  doi: 10.19439/j.sjos.2018.01.007
    Abstract ( 257 )   PDF (4558KB) ( 265 )  
    PURPOSE: To investigate the expression of Kruppel-like factor 5 (KLF5) in human periodontal ligament cells (hPDLCs) under cyclic tensile stress (CTS) and its effect on proliferation and osteogenic differentiation of hPDLCs. METHODS: hPDLCs were primarily cultured in vitro by tissue explant and enzyme digestion method. RT-PCR and Western blot were used to detect mRNA and protein expression of KLF5 in hPDLCs stimulated by 10% CTS at a frequency of 0.5 Hz for 1, 4, 8, 12 and 24 h, respectively.KLF5 was then knocked-down in hPDLCs using siRNA (si-KLF5) and its mRNA expression was determined by RT-PCR. Meanwhile, basic fibroblast growth factor (FGF2) was overexpressed in KLF-5 silenced hPDLCs by using pcDNA3.1-FGF2. After stimulation with CTS for 8 h, the proliferation of hPDLCs was assayed by CCK-8 kit, alkaline phosphatase (ALP) activity was assayed by ALP kit, the mRNA expression of Runx2 and Osterix was examined by RT-PCR, and the protein expression of Runx2, Osterix, FGF2, GSK-3β, P-GSK-3β (ser 9), β-catenin was determined by Western blot. The data were analyzed using SPSS 22.0 software package. RESULTS: Stimulation with 10% CTS time-dependently induced mRNA and protein expression of KLF5 in hPDLCs. Transfection of si-KLF5 significantly reduced the proliferation of hPDLCs, decreased ALP activity and expression of Runx2 and Osterix in hPDLCs stimulated by 10% CTS. Simultaneously, CTS-induced activation of FGF2-GSK-3β/β-catenin signaling pathway was also inhibited by si-KLF5 transfection. However, overexpression of FGF2 can partly neutralized the inhibitiory effect of si-KLF5 on proliferation and osteogenic differentiation of hPDLCs subjected to 10% CTS. CONCLUSIONS: KLF5 promotes proliferation and osteogenesis differentiation of hPDLCs subjected to cyclic tensile stress through FGF2-GSK-3β/β-catenin signaling pathway.
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    Measurement of the mesiobuccal root canal width and taper of maxillary first molars
    GOU Jing-xue, CHEN Yao, LIU Gui-hong
    2018, 27 (1):  34-38.  doi: 10.19439/j.sjos.2018.01.008
    Abstract ( 525 )   PDF (3765KB) ( 169 )  
    PURPOSE: To measure the root canal morphology of the maxillary first molars, to provide reference for clinical work. METHODS: Fifty maxillary first molars were collected and the mesiobuccal root canal taper was observed through production of transparent tooth. The mesiobuccal roots were dissected transversely from their apical foramen vertical to the long axis of the root and 0.4-mm-thick serial sections were made. Each section was observed with a stereomicroscope at 30× magnification to calculate the taper value of apical 1/3, middle third 1/3 and canal orifice 1/3. RESULTS: The width of MB1 root canal: maximum and minimum diameter of root apex was (0.38±0.12) mm and (0.34±0.16) mm, maximum and minimum diameter of root middle was (0.55±0.26) mm and (0.57±0.12) mm, maximum and minimum diameter of root collar was (1.13±0.34) mm and (0.59±0.18) mm. The width of MB2 root canal taper: maximum and minimum diameter of root apex was (0.25±0.13) mm and (0.28±0.10) mm, maximum and minimum diameter of root middle was (0.36±0.09) mm and (0.17±0.06) mm, maximum and minimum diameter of root collar was (0.79±0.23) mm and (0.23±0.17) mm. The taper of MB1 root canal: maximum and minimum diameter of root apical 1/3 was 0.03 and 0.01, maximum and minimum diameter of root middle 1/3 was 0.06 and 0.03, maximum and minimum diameter of root collar 1/3 was 0.10 and 0.09. The taper of MB2 root canal: maximum and minimum diameter of root apical 1/3 was 0.02 and - 0.01, maximum and minimum diameter of root middle 1/3 was 0.06 and 0.00, maximum and minimum diameter of root collar 1/3 was -0.02 and -0.02. CONCLUSIONS: MB1 has a larger width and taper, and root apical 1/3, middle 1/3, collar 1/3 have variable taper; While the width and taper of MB2 are small, and there will be upside down taper.
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    Isolation, identification and biological characteristics of the stem cells from mucoepidermoid carcinoma of the salivary gland
    XU Rui, YU Ming, LI Yue-ling
    2018, 27 (1):  39-42.  doi: 10.19439/j.sjos.2018.01.009
    Abstract ( 225 )   PDF (2642KB) ( 263 )  
    PURPOSE: To study the specific genes of mucoepidermoid carcinoma of mucus - like cancer cells of the salivary gland, and to further understand the mechanism of tumor stem cells. METHODS: Single cell culture of tumor cells was performed by limited dilution method. The growth of tumor stem cells was observed. The relative specific surface markers of salivary gland mucoepidermoid carcinoma stem cells were determined by immunomagnetic separation technology. The data were analyzed with SPSS 11.5 software package. RESULTS: The expression of CD133 was higher in the M3SP2 cell line, and the high expression of CD133 was significantly different(P<0.05). CONCLUSIONS: CD133 may be one of the surface specific antigen markers of M3SP2 monoclonal cell lines in salivary gland mucoepidermoid carcinoma cell line.
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    Detection and significance of IL-6 and TNF-α in patients with Graves disease and periodontitis
    WANG Yi-yue, LIN Xiao-ping
    2018, 27 (1):  43-47.  doi: 10.19439/j.sjos.2018.01.010
    Abstract ( 208 )   PDF (3691KB) ( 149 )  
    PURPOSE: To investigate the expression of IL-6 and TNF-α in gingival crevicular fluid (GCF) and serum of patients with Graves' disease(GD) and periodontitis (CP), and to analyze the correlation between Graves' disease and periodontitis. METHODS: One hundred and twenty subjects were selected and divided into 4 groups: 30 healthy participants as the control group, 30 patients with CP, 30 patients with GD, and 30 patients with GD and CP. The serum and GCF level of IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA), and clinical periodontal parameters including probing depth (PD), clinical attachment level (CAL) and sulcus bleeding indexes (SBI) were examined. The date was analyzed with SPSS19.0 software package. RESULTS: The concentration of IL-6 and TNF-α in serum and GCF of patients with GD+CP group was significantly higher than that in CP group and GD group (P<0.05). The level of IL-6 and TNF-α in serum and GCF of patients in GD+CP group and GD group was positively correlated with FT3 and FT4 (P<0.05), and the correlation between GD+CP group was significantly higher than that in GD group. CONCLUSIONS: The level of IL-6 and TNF-α in GD+CP group were significantly higher than those in CP group and GD group, indicating that there is an interaction between periodontitis and Graves' disease in immune mechanism.
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    Assessment of mental health status and plasma catecholamine levels in patients with recurrent aphthous ulcer
    HONG Chen, WANG Wen-mei, WANG Xiang, TANG Wei
    2018, 27 (1):  48-51.  doi: 10.19439/j.sjos.2018.01.011
    Abstract ( 283 )   PDF (3009KB) ( 198 )  
    PURPOSE: To investigate the relationship between mental health and recurrent aphthous ulcer (RAU), and to explore the change of plasma catecholamine level in RAU patients. METHODS: Thirty RAU patients treated in Nanjing Stomatological Hospital, Medical School of Nanjing University from February 2016 to August 2016 were selected as the experimental group, and 30 healthy people with matched age and gender were selected as the control group. Patients in both groups were asked to fill in the psychological scales for researchers to analyze their mental state, including Self-Rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS). Whole blood samples were taken from the selected subjects around 9 am to 10 am. High performance liquid chromatography (HPLC) was used to analyze the plasma catecholamine level. Statistical analysis was performed using SPSS 22.0 software package. RESULTS: The results of SAS and SDS showed that the standard scores of the experimental group were significantly higher than those of the control group (P<0.01), analysis of plasma catecholamine level showed that adrenaline level in the experimental group was significantly higher than the control group (P<0.01). Analysis of the plasma adrenaline level and standard scores of both psychological scales showed that they had significant correlation. CONCLUSIONS: There is a close relationship between the development of RAU and the mental state of patients. Meanwhile, plasma adrenaline level significantly increases in patients with RAU.
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    A prospective study of changes in oral health-related quality of life during "all-on-four" immediate restoration for edentulous individuals
    TANG Ting, ZHANG Lei, HOU Yong-fu, JIANG Ying-chun, WANG Xiang-dong, CHEN Ning
    2018, 27 (1):  52-55.  doi: 10.19439/j.sjos.2018.01.012
    Abstract ( 297 )   PDF (2696KB) ( 162 )  
    PURPOSE: This clinical study was to evaluate oral health-related quality of life (OHRQoL) changes in patients who underwent all-on-4 treatment in one or both jaws. METHODS: Eleven patients underwent placement of 4 to 6 dental implants on the basis of all-on-4 treatment concept in the maxilla, mandible or both jaws and immediate loading with acrylic interim prostheses. The prostheses were replaced after 3-6 months, and definitive prostheses with metal framework and reinforced resin facing were fixed after another 3 months or more. The subjects completed the shortened Chinese version of the Oral Health Impact Profile (OHIP-14) before surgery (T0), 1 week after interim prostheses were placed (T1) and 3 months after definitive prosthesis placement (T2). The data of 11 subjects were analyzed with Wilcoxon signed-rank test using SPSS 13.0 software package. RESULTS: The total OHIP-14 score significantly reduced only at T2 (P<0.05). "Functional limitation," "physical pain," "physical disability," and "psychological disability" scores significantly decreased at T2, and "psychological discomfort" scores also significantly dropped at T1. "Social disability" and "handicap" scores remained unchanged throughout the treatment course. CONCLUSIONS: Fixed definitive prostheses with metal framework are more effective in improving OHRQoL than fixed acrylic prostheses during all-on-4 treatments.
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    Changes of oral bacteria flora after wearing complete denture
    YU Wei-qiang, TIAN Fei, GUO Xiao-kui, ZHANG Fu-qiang, YE Dong-xia
    2018, 27 (1):  56-60.  doi: 10.19439/j.sjos.2018.01.013
    Abstract ( 337 )   PDF (3510KB) ( 196 )  
    PURPOSE: This study investigateed the influence of complete denture on oral bacteria flora. METHODS: Bateria plaque samples in oral mucosa, saliva and denture surfaces in 11 edentulous patients were collected, then the 16S rRNA gene clone libraries were constructed. Pyrosequencing was used to analyze the 16S rRNA gene V3~V4 regions, oral bacteria flora were classified and identified. RESULTS: There were 64800 sequences in complete denture-wearing subjects, Streptococcus mitis, Gemella haemolysans, Rothia mucilaginosa, Porphyromonas sp, Neisseria zoodegmatis, Granulicatella elegans, Acinetobacter baumannii, Pseudomonas citrinum, Granulicatella adiacens and Fusobacterium canifelinum were the predominant species (37416 sequences). The species of denture tissue surface were similar to these of buccal vestibule after wearing denture, and the species of denture smooth surface were similar to these of tongue ventrum and the floor of mouth. CONCLUSIONS: The effect of complete denture on oral flora is still limited, and the composition of oral flora is influenced by many other factors.
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    Comparison of clinical efficacy of CAD/CAM all -ceramic inlay and polymerid porcelain inlay in restoration of posterior Class Ⅱ cavity
    YU Miao, YAO Li-li, WANG Chun-hong, WANG Mian, FAN Cong
    2018, 27 (1):  61-64.  doi: 10.19439/j.sjos.2018.01.014
    Abstract ( 803 )   PDF (2888KB) ( 169 )  
    PURPOSE: To compare the clinical efficacy of CAD/CAM all-ceramic inlay and polymerid porcelain inlay in restoring Class Ⅱ cavity of posterior teeth. METHODS: Ninety-seven patients with 100 posterior teeth of ClassⅡ cavity were recruited in this randomized control trial; Among them, 50 patients were grouped into CAD/CAM all-ceramic inlays and 47 patients were grouped into Ceramage polymerid porcelain inlay. According to the modified USPHS criteria, the incidence of postoperative sensitivity, prosthesis fracture, prosthesis falling off, and edge coloration were evaluated 12 months and 24 months after restoration. X2 test and Wilcoxon rank sum test were used for statistical analysis using SPSS 13.0 software package. RESULTS: Restoration in the 2 groups were successful, there was no significant difference at 12 months (P>0.05). Postoperative sensitivity and the incidence of prosthesis falling off in both groups were not significantly different (P>0.05); however, the number of prosthesis fracture of the polymerid porcelain was lower than that of the CAD/CAM all-ceramic inlays (P<0.05). The incidence of edge coloration of CAD/CAM all-ceramic inlays was lower than that of the polymerid porcelain at 24-month follow-up (P<0.05). CONCLUSIONS: Restoration with polymerid porcelain is more likely to have a higher success rate than those with CAD/CAM all-ceramic inlays. Patients undergoing CAD/CAM all-ceramic inlays have a lower incidence of edge coloration, compared with those undergoing polymerid porcelain.
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    Evaluation of the effectiveness of cold-light whitening technique on tetracycline pigmentation teeth and aged extrinsic stain teeth
    CHENG Hui-juan, GENG Yi, ZHAO Jun
    2018, 27 (1):  65-67.  doi: 10.19439/j.sjos.2018.01.015
    Abstract ( 202 )   PDF (2432KB) ( 236 )  
    PURPOSE: To evaluate the clinical effectiveness of cold-light whitening technique on tetracycline pigmentation teeth and aged extrinsic stain teeth. METHODS: One hundred and fifty-two cases bleached by cold-light whitening technique were included in this study. The patients' teeth were divided into 3 groups: aged extrinsic stain teeth, mild tetracycline pigmentation teeth and moderate tetracycline pigmentation teeth. Visual determination of shade and Olympus crystaleye spectrophotometer were used to measure the tooth discoloration after bleaching. SPSS17.0 software package was used for statistical analysis. RESULTS: Visual determination showed that the efficiency of aged extrinsic stain teeth was significantly higher than that of mild and moderate tetracycline teeth group (P<0.05). Colorimetric method showed that the △E of moderate tetracycline teeth was significantly lower than aged extrinsic teeth and mild tetracycline teeth (P<0. 05) . The △E of cervix changed more significantly than that of central and incisal edge in both mild and moderate tetracycline teeth group (P<0. 05). CONCLUSIONS: The effect of cold-light whitening technique on aged extrinsic stain teeth is better than that of the tetracycline pigmentation teeth. Cold-light whitening technique can improve pigmentation of tetracycline teeth cervix.
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    Clinical evaluations of transitional treatment of pulpitis during pregnancy in 60 female patients
    FU Shuang, ZHANG Yan, SONG Wen-bin
    2018, 27 (1):  68-72.  doi: 10.19439/j.sjos.2018.01.016
    Abstract ( 271 )   PDF (3678KB) ( 243 )  
    PURPOSE: The present study was aimed to clinically evaluate transitional treatment of pulpitis during pregnancy. METHODS: Sixty pregnant women with pulpitis were divided into the first, second and third trimester. They were anesthetized locally using STA system combined with psychological intervention during treatment. The teeth were given one-appointment root canal preparation and filled with vitapex pasta. Normal obturation of the root canal was conducted after delivery. The effects of dental treatment, pregnancy outcomes and neonatal health status were observed. Data of the survey were analyzed by SPSS 20.0 software package, including Student,s t test and chi-square test. RESULTS: The symptoms of pulpitis were significantly improved 24h after treatment,and the effective rate was 96.7%. The difference of VAS before and after treatment was statistically significant (P<0.05). Patients had no discomfort till the end of pregnancy,and the success rate was 98.3%. No adverse pregnancy outcomes were observed after treatment during different periods of pregnancy. Neonatal health was good. Significant differences were not found in birth gestational age, body weight, malformations, and neonatal complications between three treatment periods (P>0.05). CONCLUSIONS: Transitional treatment of pulpitis during pregnancy is a safe and effective procedure and worthy of wide use clinically.
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    The value of ultrasonic elastography in diagnosis of salivary gland lesions in patients with Sjögren's syndrome
    ZHANG Wei-qian, GONG Xia, XIONG Ping
    2018, 27 (1):  73-78.  doi: 10.19439/j.sjos.2018.01.017
    Abstract ( 266 )   PDF (4818KB) ( 207 )  
    PURPOSE: To evaluate the value of strain rate ratio (SR) and acoustic radiation force impulse (ARFI) imaging in the diagnosis of salivary glands lesions in patients with Sjögren's syndrome (SS). METHODS: Thirty-six outpatients with SS in the Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from January 2017 to July 2017 were collected. All patients were diagnosed as SS according to American-European Consensus Group criteria of 2002. 25 healthy controls were chosen as control. The bilateral parotid gland and submandibular gland were examined by strain rate ratio and ARFI imaging, and the SR and shear wave velocity (SWV) value were obtained. The differences in SR or SWV values between the left and right sides of the parotid gland or submandibular gland and between SS group and healthy control group were analyzed using SPSS 22.0 software package. RESULTS: There was no significant difference in SR and SWV value between left and right parotid gland and submandibular gland in the SS group and the healthy control group (P>0.05). There were significant differences in SR values between bilateral parotid gland and submandibular gland (P<0.05).There was significant difference in SWV values between the two groups (P<0.05). With 0.8962 as the cutoff value, the sensitivity of SS diagnosis in the parotid gland was 77.8% by using SR, the specificity was 88.0% and the area under the curve was 0.844. With 0.8987 as the cutoff value, the sensitivity of SS diagnosis in the submandibular gland was 47.22%,the specificity was 84.0% and the area under the curve was 0.652. By using ARFI technique, the sensitivity of SS diagnosis in the parotid gland was 91.7%, the specificity was 88.0%, and the area under the curve was 0.943, when the cutoff value was 1.6288. With 1.8788 as the cutoff value, the sensitivity of SS diagnosis in the submandibular gland was 44.4%, the specificity was 96.0%, and the area under the curve was 0.614. CONCLUSIONS: SR and ARFI imaging method can provide SR and SWV value of salivary gland to quantify the hardness of salivary glands in SS, which has great value in diagnosis of SS.
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    A study on effects of unilateral maxillary canine impaction on the dento-maxillofacial three-dimensional structure
    LIU An-qi, HUANG Jia-liang, QIAN Yu-fen
    2018, 27 (1):  79-84.  doi: 10.19439/j.sjos.2018.01.018
    Abstract ( 339 )   PDF (4423KB) ( 292 )  
    PURPOSE: To investigate the three-dimensional structure characteristics of unilateral impacted teeth by cone-beam CT (CBCT), and to evaluate the risk factors for upper impacted teeth, so as to provide evidence for early clinical intervention in orthodontics. METHODS: Thirty patients with unilateral maxillary canine impaction were included. Their CBCT images were measured by three-dimensional reconstruction of Romexis software. The differences of the three-dimensional structure characteristics between two sides were measured and compared with SPSS17.0 software package. Paired t test and regression analysis of related data were performed. RESULTS: Significant difference in inclination of both canine and lateral incisor was found between impacted side and normal eruption side, with canine mesially inclined and lateral incisor distally inclined (P<0.001). Volumes of the canines were significantly bigger and those of the lateral incisor were significantly smaller on the impacted side compared with the normal eruption side (P<0.05). The occurrence of impacted canine increased with the increase of the volume of the canine and decrease of the volume of the lateral incisor. Significant difference in arch length and arch width in canine and premolar area was found between impacted side and normal eruption side (P<0.001). There was no significant difference in width in molar area between both sides(P>0.05). There was no significant difference in arch length in canine, premolar and molar region (P>0.05). CONCLUSIONS: Maxillary impacted canines can lead to three-dimensional abnormalities of the ipsilateral teeth and alveolar bone, the severity of maxillary impacted canine is closely related to surrounding dental structural abnormalities, suggesting that maxillary impacted canines can be predicted, early diagnosed, and early effectively intervened as well.
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    Effect of dexmedetomidine combined with sevoflurane for general anesthesia during dental treatment in pediatric patients
    CHAI Dong-dong, JI Jun
    2018, 27 (1):  85-88.  doi: 10.19439/j.sjos.2018.01.019
    Abstract ( 307 )   PDF (2875KB) ( 224 )  
    PURPOSE: To evaluate the effect of dexmedetomidine combined with sevoflurane for general anesthesia during dental treatment in pediatric patients. METHODS: One hundred and twenty pediatric patients undergoing dental treatment were randomly divided into sevoflurane combined dexmedetomidine group(D group)and sevoflurane group (S group), 60 children in each group. Children in group D inhaled 4%-6% sevoflurane and intravenously received fentanyl 1 μg/kg, midazolam 0.1 mg, propofol 2 mg/kg, rocuronium 0.3 mg/kg, then tracheal intubation was performed when eyelash reflex disappeared, the density of sevoflurane was reduced to 2%-3% for maintenance. When the treatment started, 9-12 mg/(kg·h) of propofol was pumped, and 1 μg/kg of dexmedetomidine was pumped for 15 minutes, then the dose was reduced to 0.5 μg/(kg·h) until the end of treatment. Children in group S didn't receive dexmedetomidine pumped. During the procedure, HR, MAP, and Ramsay scale were recorded at baseline (T1), loss of eyelash reflex (T2), after dexmedetomidine was pumped for 10 mins (T3), operation initiation (T4) and the end of operation (T5). The operation time, recover and extubation time, pediatric anesthesia emergence delirium scale (PAED) in pediatric anesthesia care unit (PACU) were recorded. SPSS 20.0 software package was used for statistical analysis. RESULTS: There was no significant difference in operation time between the two groups. At T3-T5, MAP and HR were significantly lower, but Ramsay scale was significantly higher in group D than in group S (P<0.05). The recover and extubation time were significantly longer in group D (P<0 .05). In PACU, PAED scale was significantly lower in group D than in group S (P<0.05). CONCLUSIONS: Dexmedetomidine combined with sevoflurane not only stabilize hemodynamic parameters, but also reduce the impact on respiration during dental treatment in pediatric patients.
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    Clinical observation of the efficacy of Hybrid coat combined with composite resin for restoring hypersensitive wedge-shaped defect
    WANG Yi, HE Hong
    2018, 27 (1):  89-92.  doi: 10.19439/j.sjos.2018.01.020
    Abstract ( 326 )   PDF (3021KB) ( 216 )  
    PURPOSE: The purpose of this study was to explore the effect of Hybrid-coat combined with composite resin filling on reducing hypersensitivity of dental wedge-shaped defect. METHODS: Seventy cases, 159 teeth with sensitive wedge-shaped defects were selected. The patients were randomly divided into 2 groups and treated as below: 35 cases, 81 teeth in group A were treated with Hybrid-coat combined with composite resin; 35 cases,78 teeth in group B were treated with composite resin directly. After treatments, the effective rates under clinical examination standard (immediately, 1, 3 and 6 months after filling) in both groups were collected and statistically analysed with SPSS 13.0 software package. RESULTS: In group A, one and 2 teeth fillings fell off 3 months and 6 months after treatments ,respectively. In group B, 2 teeth fillings fell off 6 months after operation. There was no significant difference between the two groups. The effective rate of desensitization was 91.36%, 88.89%, 79.01% and 70.37%, respectively, which was higher than those of group B (78.21%, 76.92%, 69.23% and 61.54%, respectively). There was significant difference between the two groups immediately and 1 months after treatment (P<0.05), but there was no significant difference between the two groups 3 months and 6 months after treatment. CONCLUSIONS: Hybrid-coat combined with composite resin is effective in reducing sensitivity of wedge-shaped defects.
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    Evaluation of the effect of concentrated growth factor in oral rehabilitation
    LU Jun, SUN Chang-an
    2018, 27 (1):  93-95.  doi: 10.19439/j.sjos.2018.01.021
    Abstract ( 527 )   PDF (2296KB) ( 189 )  
    PURPOSE: To investigate the clinical effect of concentrated growth factor (CGF) in oral cavity repair. METHODS: From February 2013 to February 2016 in our hospital, 60 patients with insufficient bone underwent dental implantation. CGF was used alone in 27 patients (CGF group), CGF was used with autologous bone or bone material in 33 patients (CGF mixed group). Bone repair was observed and evaluated. SPSS19.0 software package was used for statistical analysis. RESULTS: Wound healing time in CGF group and CGF mixed group (8.43±0.72) d and (8.04±0.98) d, wound healing rate was 92.60% in CGF group and 90.91% in CGF group, the difference was not statistically significant (P>0.05); Implant success rate in CGF group and CGF mixed group was 92.31% and 96.36% , respectively. The difference was not statistically significant (P>0.05). CONCLUSIONS: CGF has good effect in repair of bone defect, and the long-term effect is stable. It is a simple and safe material for bone regeneration.
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    Clinical analysis of muscle function intensifier in treatment of 40 patients with maxillary protrusion
    XIE Xue-mei, XIE Xing-qian
    2018, 27 (1):  96-99.  doi: 10.19439/j.sjos.2018.01.022
    Abstract ( 293 )   PDF (2966KB) ( 147 )  
    PURPOSE: To investigate the clinical effects of combined muscle functions intensifier and straight wire appliance in the treatment of maxillary protrusion. METHODS: Forty patients with maxillary protrusion were randomly divided into 2 groups (group A and group B). Straight wire appliance and muscle function intensifier were used in group A, while patients in group B received straight wire appliance only. X-ray projection was conducted to measure relative maxillary index (labial inclination of upper or lower incisor, SNB angle, ANB angle, FMA, maxillary plane angle, NA-PA, U1-FH, U1-PP, forward moving distance of U6) before and 6 months after treatment. The efficacy was analyzed and evaluated using SPSS 20.0 software package. RESULTS: After correction, labial inclination of upper or lower incisor, SNB angle, ANB angle, FMA, maxillary plane angle, NA-PA, U1-FH, U1-PP changed significantly in both groups (P<0.05), but patients in group A achieved more incisor adduction and less forward moving distance of U6, with significant difference between the two groups (P<0.05). CONCLUSIONS: Most patients with maxillary protrusion have a bad habit of mouth breathing. Muscle function intensifier is used only at night and can relieve the patient's mouth breathing, speed up the movement of teeth, and reduce the loss of anchorage and ultimately, improve the correction efficiency and clinical effect of patients with maxillary protrusion.
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    Systemic Review
    Clinicopathological and prognostic significance of PD-L1 expression in oral squamous cell carcinoma: a meta analysis
    ZOU Bo, XU Kai, LIU Xian-bin, FAN Qing-chun, LIU Jian-lin, YUAN Dao-ying
    2018, 27 (1):  100-105.  doi: 10.19439/j.sjos.2018.01.023
    Abstract ( 473 )   PDF (4308KB) ( 268 )  
    PURPOSE: To determine the prognostic role of high PD-L1 in patients with oral squamous cell carcinoma. METHODS: Electronic databases, such as PubMed, Embase and Cochrane library, were searched to identify studies evaluating PD-L1 expression and overall survival (OS) in these patients. RevMan 5.3 software was used for meta-analysis. RESULTS: A total of 12 studies that involved 1595 patients were included. Pooled hazard ratio (HR) 1.02 (95%CI= 0.93-1.11,P=0.71) indicated that the association between PD-L1 expression and overall survival (OS) was not significant. The pooled odds ratios (ORs) indicated that PD-L1 expression was associated with gender (OR=0.64, 95%CI=0.48-0.85, P=0.002), differentiation (OR=0.58, 95%CI=0.37-0.90, P=0.01) and HPV infection (OR=1.91, 95%CI=1.13-3.23, P=0.02). However, PD-L1 had no correlation with tumour size, and lymph node status. CONCLUSIONS: PD-L1 expression may not be an independent predictor of prognosis of patients with OSCC. Well-designed large cohort studies are needed to confirm these findings.
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    Dental Nursing
    Perioperative nursing of zygomatic implant surgery for patients with severe periodontitis
    BO Fang, GU Wei, LING Xiao-wan, WU Yi-qun, WANG Yue-ping
    2018, 27 (1):  106-109.  doi: 10.19439/j.sjos.2018.01.024
    Abstract ( 315 )   PDF (3481KB) ( 339 )  
    PURPOSE: To analyze the perioperative nursing characteristics about severe periodontitis patients with immediate zygomatic implantation, and to summarize the clinical nursing experience. METHODS: Preoperatively, all patients received psychological counseling, oral hygiene instruction, and periodontal care with necessary means after physical examination. Cooperative procedures were preformed between nurses and surgeon during operation. After operation, nursing interventions were given including wound care, pain control, ice compression, appropriate diet and close follow-up. RESULTS: All patients were successfully operated without serious complications. Favorable implant primary stability was achieved in each patient. No patient developed wound dehiscence, one patient had wound pain and one patient had minor facial swelling. Temporary restoration was conducted to restore occlusal relationship and masticatory function within 48 hours. CONCLUSIONS: Comprehensive nursing intervention during perioperative period is necessary and deserves to be applied for patients with severe periodontitis who are treated with immediate zygomatic implant surgery.
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    Dental Education
    Evaluation of modified scene simulation teaching of endodontics in pre -clinical practice
    GE Gentana, JIN Wu-long, LIU Shuang-feng
    2018, 27 (1):  110-112.  doi: 10.19439/j.sjos.2018.01.025
    Abstract ( 188 )   PDF (2090KB) ( 185 )  
    PURPOSE: To evaluate the effect of modified scene simulation teaching of endodontics in pre-clinical teaching. METHODS: Forty students were randomly divided into the experimental group and control group, there were 20 students in each group. Students in the experimental group were taught with modified scene simulation teaching method, while students in the control group were taught with traditional teaching method. The teaching quality was compared with SPSS 18.0 software package. RESULTS: The score of the experimental group was significantly higher than the control group in history taking, medical records writing and practical performance(P<0.05). CONCLUSIONS: The teaching effect of modified scene simulation teaching in pre-clinical teaching is better than traditional teaching method. It makes students better grasp the clinical medical essentials and feels the patient's feelings, as well as help the students to improve their subjective initiative of learning.
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