低强度高频振动对人牙周膜干细胞成骨分化能力的影响

doi:10.19439/j.sjos.2019.05.006

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (5): 478-483.doi: 10.19439/j.sjos.2019.05.006

低强度高频振动对人牙周膜干细胞成骨分化能力的影响

白坤宏¹, 胡诚¹, 李青青², 王菲¹

1.西安交通大学口腔医院正畸科, 西安交通大学口腔医学院陕西省颅颌面精准医学研究重点实验室,陕西西安 710004;
2.深圳市同步齿科医疗股份有限公司同步口腔门诊部,广东深圳 518000

收稿日期:2018-08-17 出版日期:2019-10-25 发布日期:2019-12-11
通讯作者: 王菲,E-mail: wf751022@mail.xjtu.edu.cn
作者简介:白坤宏（1991-）,男,硕士,E-mail: bkh1991@163.com
基金资助:
陕西省牙颌疾病临床医学研究中心开放课题（2017YHJB01）

Effect of low-magnitude high frequency vibration on osteogenic differentiation of human periodontal ligament stem cells

BAI Kun-hong¹, HU Cheng¹, LI Qing-qing², WANG Fei¹

1.Department of Orthodontics, Stomatological Hospital of Xi'an Jiaotong University, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology, Xi'an Jiaotong University.Xi'an 710004,Shaanxi Province;
2.Shenzhen Top Dental.Shenzhen 518000,Guangdong Province,China

Received:2018-08-17 Online:2019-10-25 Published:2019-12-11

摘要/Abstract

摘要： 目的研究低强度高频振动（low-magnitude high frequency vibration,LMHFV）对人牙周膜干细胞（human periodontal ligament stem cells,hPDLSCs）增殖、迁移、成骨分化能力的影响。方法体外分离培养hPDLSCs;流式细胞术检测间充质干细胞表面标志物;加载LMHFV（加速度=0.3 g,频率=40 Hz,时间=15 min/24 h）刺激后,采用CCK-8试剂盒检测细胞增殖能力,通过细胞划痕实验检测细胞迁移能力;通过qRT-PCR、Western免疫印迹检测成骨相关基因、蛋白表达水平,通过茜素红染色检测细胞成骨分化能力。采用SPSS 21.0软件包对数据进行统计学分析。结果加载振动刺激后,hPDLSCs的增殖能力增强,迁移能力上升;RUNX2、ALP、Col-1、OCN的mRNA表达量和蛋白表达量均上升,茜素红染色结果与qRT-PCR、Western免疫印迹结果一致。结论 LMHFV可提高hPDLSCs的增殖、迁移能力和成骨分化能力。

关键词: 振动, 牙周膜干细胞, 增殖, 成骨分化

Abstract: PURPOSE: To investigate the effect of low-magnitude high frequency vibration (LMHFV) on proliferation, migration ability and osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs). METHODS: hPDLSCs were isolated from premolar and randomized into vibration culture group (magnitude：0.3 g; frequency：40 Hz; time：15 min/24 h) and static culture group. CCK-8 was used to identity the proliferation of hPDLSCs. Wound-healing assay was used to evaluate migration ability of hPDLSCs. The osteogenesis gene expression was analyzed by RT-PCR, and the osteogenesis protein expression was analyzed by Western blot. The osteogenesis differentiation capability was evaluated by alizarin red staining. The data were analyzed using SPSS 21.0 software package. RESULTS: After LMHFV, the proliferation and migration ability of hPDLSCs were increased. The expression level of RUNX2, ALP, Col-1, and OCN was significantly augmented under LMHFV. Alizarin red staining and Western blot proved the same trend. CONCLUSIONS: The results demonstrate that LMHFV can promote hPDLSCs proliferation, migration ability and osteogenic differentiation.

Key words: Vibration, Periodontal ligament stem cells, Proliferation, Osteogenic differentiation

中图分类号:

R781.4

白坤宏, 胡诚, 李青青, 王菲. 低强度高频振动对人牙周膜干细胞成骨分化能力的影响[J]. 上海口腔医学, 2019, 28(5): 478-483.

BAI Kun-hong, HU Cheng, LI Qing-qing, WANG Fei. Effect of low-magnitude high frequency vibration on osteogenic differentiation of human periodontal ligament stem cells[J]. Shanghai Journal of Stomatology, 2019, 28(5): 478-483.

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低强度高频振动对人牙周膜干细胞成骨分化能力的影响

Effect of low-magnitude high frequency vibration on osteogenic differentiation of human periodontal ligament stem cells

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