上海口腔医学

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (5): 467-471.doi: 10.19439/j.sjos.2019.05.004

• 论著 • 上一篇    下一篇

用于三维打印咬合板的聚碳酸酯材料性能研究

林瑞, 郁春华*, 孙健*   

  1. 上海交通大学医学院附属第九人民医院·口腔医学院 口腔修复科,国家口腔疾病临床医学研究中心, 上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011
  • 收稿日期:2018-09-20 出版日期:2019-10-25 发布日期:2019-12-11
  • 通讯作者: 郁春华,E-mail:yuchunhua656663@163.com;孙健, E-mail:doctorsunjian74@aliyun.com。*共同通信作者
  • 作者简介:林瑞(1993-),女,硕士,E-mail:dianelin3@163.com
  • 基金资助:
    上海市科委医学引导类项目（19411962100）; 上海高校高峰高原学科建设项目; 上海交通大学医学院附属第九人民医院院基金（2016-6）

Mechanical properties and cytotoxicity of three-dimensional printing polycarbonate for occlusal splints

LIN Rui, YU Chun-hua, SUN Jian   

  1. Department of Prosthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;National Clinical Research Center for Oral Diseases;Shanghai Key Laboratory of Stomatology &Shanghai Research Institute of Stomatology.Shanghai 200011, China
  • Received:2018-09-20 Online:2019-10-25 Published:2019-12-11

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摘要： 目的 检测一种三维打印聚碳酸酯咬合板材料的机械性能和细胞毒性,尝试利用该材料三维打印咬合板。方法 分别检测三维打印聚碳酸酯材料（PC-plus）和常规制作咬合板的聚甲基丙烯酸甲酯（PMMA）的挠曲强度、挠曲弹性模量、显微硬度、吸水值和溶水值。小鼠成纤维细胞（L-929）经PC-plus浸提液培养后,观察细胞形态,CCK-8法检测细胞活性。通过三维打印技术使用PC-plus制作咬合板。采用SPSS 24.0软件包对数据进行统计学分析。结果 三维打印PC-plus的挠曲强度值为 89.4~109.8 MPa,挠曲弹性模量值为1939.4~2470.9 GPa,显微硬度值为 15.6~24.7 MPa,吸水值为2.43~11.42 μg/mm3,溶水值为 0.11~0.30 μg/mm3。PMMA材料的挠曲强度值为 75.2~88.4 MPa,挠曲弹性模量值为1349.2~2470.2 GPa,显微硬度值为 17.5~35.3 MPa,吸水值为12.80~16.16 μg/mm3,溶水值为 4.74~7.44 μg/mm3。三维打印PC-plus组与PMMA基托树脂组之间的差异均有统计学意义（P<0.05）。培养期细胞贴壁生长良好,细胞形态正常,各实验组的毒性评级为 0~1 级。通过数字化设计和制作,成功制成咬合板。结论 三维打印聚碳酸酯材料的机械性能好,无明显细胞毒性,具备口内应用的生物安全基础,具有良好的临床应用前景。

关键词: 三维打印, 聚碳酸酯, 咬合板, 机械性能, 细胞毒性

Abstract: PURPOSE: To evaluate the mechanical properties and cytotoxicity of a 3D printing polycarbonate material used in digital occlusal splints, and to use this material to manufacture a splint with CAD/CAM technology. METHODS: Specimen of two different materials, 3D printing polycarbonate(PC-plus) and transparent base resin (PMMA) were processed. The flexural strength, elastic modulus, microhardness, hygroscopicity and water-solubility of these materials then were evaluated. According to the standard of GBT16886.5-2003, cell culture and cytotoxicity test in vitro were conducted to evaluate the target materials on the morphology, growth and proliferation of cultured cells (L929). By using 3D printing technology, a splint was made of this 3D printing polycarbonate material. Statistical analysis was performed using SPSS 24.0 software package. RESULTS: For the group of PC-plus, the flexural strength ranged from 89.4 to 109.8 MPa, the elastic modulus from 1939.4 to 2470.9 GPa, the microhardness from 15.6 to 24.7 MPa, hygroscopicity from 2.43 to 11.42 μg/mm3 and water-solubility from 0.11 to 0.30 μg/mm3. For PMMA group, the flexural strength ranged from 75.2 to 88.4 MPa, the elastic modulus from 1349.2 to 2470.2 GPa, the microhardness from 17.5 to 35.3 MPa, hygroscopicity from 12.80 to 16.16 μg/mm3 and water-solubility from 4.74 to 7.44 μg/mm3. The difference between 3D printing PC-plus group and PMMA base resin group was statistically significant (P<0.05). L929 cells showed normal morphology and proliferation increased with culture time. The toxicity grade of all groups was 0-1, and the 3D printing splint was made successfully. CONCLUSIONS: The polycarbonate material for 3D printing has adequate mechanical properties and biocompatibility to meet the requiement of clinical application.

Key words: Three-dimensional printing, Polycarbonate, Occlusal splints, Mechanical properties, Cytotoxicity

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